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Resveratrol inhibits plasma membrane Ca(2+)-ATPase inducing an increase in cytoplasmic calcium
Plasma membrane Ca(2+)-ATPase (PMCA) plays a vital role in maintaining cytosolic calcium concentration ([Ca(2+)](i)). Given that many diseases have modified PMCA expression and activity, PMCA is an important potential target for therapeutic treatment. This study demonstrates that the non-toxic, natu...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613515/ https://www.ncbi.nlm.nih.gov/pubmed/28955914 http://dx.doi.org/10.1016/j.bbrep.2016.06.019 |
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author | Peterson, Joshua Allen Oblad, Richard Vernon Mecham, Jeffrey Chad Kenealey, Jason Donald |
author_facet | Peterson, Joshua Allen Oblad, Richard Vernon Mecham, Jeffrey Chad Kenealey, Jason Donald |
author_sort | Peterson, Joshua Allen |
collection | PubMed |
description | Plasma membrane Ca(2+)-ATPase (PMCA) plays a vital role in maintaining cytosolic calcium concentration ([Ca(2+)](i)). Given that many diseases have modified PMCA expression and activity, PMCA is an important potential target for therapeutic treatment. This study demonstrates that the non-toxic, naturally-occurring polyphenol resveratrol (RES) induces increases in [Ca(2+)](i) via PMCA inhibition in primary dermal fibroblasts and MDA-MB-231 breast cancer cells. Our results also illustrate that RES and the fluorescent intracellular calcium indicator Fura-2, are compatible for simultaneous use, in contrast to previous studies, which indicated that RES modulates the Fura-2 fluorescence independent of calcium concentration. Because RES has been identified as a PMCA inhibitor, further studies may be conducted to develop more specific PMCA inhibitors from RES derivatives for potential therapeutic use. |
format | Online Article Text |
id | pubmed-5613515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-56135152017-09-27 Resveratrol inhibits plasma membrane Ca(2+)-ATPase inducing an increase in cytoplasmic calcium Peterson, Joshua Allen Oblad, Richard Vernon Mecham, Jeffrey Chad Kenealey, Jason Donald Biochem Biophys Rep Research Article Plasma membrane Ca(2+)-ATPase (PMCA) plays a vital role in maintaining cytosolic calcium concentration ([Ca(2+)](i)). Given that many diseases have modified PMCA expression and activity, PMCA is an important potential target for therapeutic treatment. This study demonstrates that the non-toxic, naturally-occurring polyphenol resveratrol (RES) induces increases in [Ca(2+)](i) via PMCA inhibition in primary dermal fibroblasts and MDA-MB-231 breast cancer cells. Our results also illustrate that RES and the fluorescent intracellular calcium indicator Fura-2, are compatible for simultaneous use, in contrast to previous studies, which indicated that RES modulates the Fura-2 fluorescence independent of calcium concentration. Because RES has been identified as a PMCA inhibitor, further studies may be conducted to develop more specific PMCA inhibitors from RES derivatives for potential therapeutic use. Elsevier 2016-06-30 /pmc/articles/PMC5613515/ /pubmed/28955914 http://dx.doi.org/10.1016/j.bbrep.2016.06.019 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Peterson, Joshua Allen Oblad, Richard Vernon Mecham, Jeffrey Chad Kenealey, Jason Donald Resveratrol inhibits plasma membrane Ca(2+)-ATPase inducing an increase in cytoplasmic calcium |
title | Resveratrol inhibits plasma membrane Ca(2+)-ATPase inducing an increase in cytoplasmic calcium |
title_full | Resveratrol inhibits plasma membrane Ca(2+)-ATPase inducing an increase in cytoplasmic calcium |
title_fullStr | Resveratrol inhibits plasma membrane Ca(2+)-ATPase inducing an increase in cytoplasmic calcium |
title_full_unstemmed | Resveratrol inhibits plasma membrane Ca(2+)-ATPase inducing an increase in cytoplasmic calcium |
title_short | Resveratrol inhibits plasma membrane Ca(2+)-ATPase inducing an increase in cytoplasmic calcium |
title_sort | resveratrol inhibits plasma membrane ca(2+)-atpase inducing an increase in cytoplasmic calcium |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613515/ https://www.ncbi.nlm.nih.gov/pubmed/28955914 http://dx.doi.org/10.1016/j.bbrep.2016.06.019 |
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