Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?

Mammalian organs comprise a variety of cells that interact with each other and have distinct biological roles. Access to evaluate and perturb intact biological systems at the cellular and molecular levels is essential to fully understand their functioning in normal and diseased conditions, yet techn...

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Autores principales: Greenbaum, Alon, Jang, Min J., Challis, Collin, Gradinaru, Viviana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Question and Answer
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613628/
https://www.ncbi.nlm.nih.gov/pubmed/28946882
http://dx.doi.org/10.1186/s12915-017-0421-3
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author Greenbaum, Alon
Jang, Min J.
Challis, Collin
Gradinaru, Viviana
author_facet Greenbaum, Alon
Jang, Min J.
Challis, Collin
Gradinaru, Viviana
author_sort Greenbaum, Alon
collection PubMed
description Mammalian organs comprise a variety of cells that interact with each other and have distinct biological roles. Access to evaluate and perturb intact biological systems at the cellular and molecular levels is essential to fully understand their functioning in normal and diseased conditions, yet technical limitations have constrained most research to small pieces of tissue. Tissue clearing and optogenetics can help overcome this hurdle: tissue clearing affords optical interrogation of whole organs at the molecular level, and optogenetics enables the scalable control and measurement of cellular activity with light. In this Q&A, we delineate recent advances and practical challenges associated with these two techniques when applied body-wide.
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spelling pubmed-56136282017-10-11 Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology? Greenbaum, Alon Jang, Min J. Challis, Collin Gradinaru, Viviana BMC Biol Question and Answer Mammalian organs comprise a variety of cells that interact with each other and have distinct biological roles. Access to evaluate and perturb intact biological systems at the cellular and molecular levels is essential to fully understand their functioning in normal and diseased conditions, yet technical limitations have constrained most research to small pieces of tissue. Tissue clearing and optogenetics can help overcome this hurdle: tissue clearing affords optical interrogation of whole organs at the molecular level, and optogenetics enables the scalable control and measurement of cellular activity with light. In this Q&A, we delineate recent advances and practical challenges associated with these two techniques when applied body-wide. BioMed Central 2017-09-25 /pmc/articles/PMC5613628/ /pubmed/28946882 http://dx.doi.org/10.1186/s12915-017-0421-3 Text en © Gradinaru et al. 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Question and Answer
Greenbaum, Alon
Jang, Min J.
Challis, Collin
Gradinaru, Viviana
Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?
title Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?
title_full Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?
title_fullStr Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?
title_full_unstemmed Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?
title_short Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?
title_sort q&a: how can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?
topic Question and Answer
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613628/
https://www.ncbi.nlm.nih.gov/pubmed/28946882
http://dx.doi.org/10.1186/s12915-017-0421-3
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