Proteolytic activity assayed by subcellular localization switching of a substrate

An approach to assay proteolytic activity in vivo by altering the subcellular localization of a labelled substrate was demonstrated. The assay included a protein shuttling between different cellular compartments and a site-specific recombinant protease. The shuttle protein used was the human immunod...

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Detalles Bibliográficos
Autores principales: Szilvay, Anne-Marie, Sarria, Shirley Vanessa, Mannelqvist, Monica, Aasland, Rein, Furnes, Clemens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613695/
https://www.ncbi.nlm.nih.gov/pubmed/28955937
http://dx.doi.org/10.1016/j.bbrep.2016.07.011

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