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Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells

Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human plate...

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Autores principales: Mangum, Lauren H., Natesan, Shanmugasundaram, Stone, Randolph, Wrice, Nicole L., Larson, David A., Florell, Kyle F., Christy, Barbara A., Herzig, Maryanne C., Cap, Andrew P., Christy, Robert J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613713/
https://www.ncbi.nlm.nih.gov/pubmed/29138638
http://dx.doi.org/10.1155/2017/7108458
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author Mangum, Lauren H.
Natesan, Shanmugasundaram
Stone, Randolph
Wrice, Nicole L.
Larson, David A.
Florell, Kyle F.
Christy, Barbara A.
Herzig, Maryanne C.
Cap, Andrew P.
Christy, Robert J.
author_facet Mangum, Lauren H.
Natesan, Shanmugasundaram
Stone, Randolph
Wrice, Nicole L.
Larson, David A.
Florell, Kyle F.
Christy, Barbara A.
Herzig, Maryanne C.
Cap, Andrew P.
Christy, Robert J.
author_sort Mangum, Lauren H.
collection PubMed
description Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human platelet lysate (hPL) is one potential xeno-free, alternative supplement for use in ASC culture. In this study, adipogenic and osteogenic differentiation in media supplemented with 10% FBS or 10% hPL was compared in human ASCs derived from abdominoplasty (HAP) or from adipose associated with debrided burned skin (BH). Most (95–99%) cells cultured in FBS were stained positive for CD73, CD90, CD105, and CD142. FBS supplementation was associated with increased triglyceride content and expression of adipogenic genes. Culture in hPL significantly decreased surface staining of CD105 by 31% and 48% and CD142 by 27% and 35% in HAP and BH, respectively (p < 0.05). Culture of BH-ASCs in hPL also increased expression of markers of osteogenesis and increased ALP activity. These data indicate that application of ASCs for wound healing may be influenced by ASC source as well as culture conditions used to expand them. As such, these factors must be taken into consideration before ASCs are used for regenerative purposes.
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spelling pubmed-56137132017-11-14 Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells Mangum, Lauren H. Natesan, Shanmugasundaram Stone, Randolph Wrice, Nicole L. Larson, David A. Florell, Kyle F. Christy, Barbara A. Herzig, Maryanne C. Cap, Andrew P. Christy, Robert J. Stem Cells Int Research Article Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human platelet lysate (hPL) is one potential xeno-free, alternative supplement for use in ASC culture. In this study, adipogenic and osteogenic differentiation in media supplemented with 10% FBS or 10% hPL was compared in human ASCs derived from abdominoplasty (HAP) or from adipose associated with debrided burned skin (BH). Most (95–99%) cells cultured in FBS were stained positive for CD73, CD90, CD105, and CD142. FBS supplementation was associated with increased triglyceride content and expression of adipogenic genes. Culture in hPL significantly decreased surface staining of CD105 by 31% and 48% and CD142 by 27% and 35% in HAP and BH, respectively (p < 0.05). Culture of BH-ASCs in hPL also increased expression of markers of osteogenesis and increased ALP activity. These data indicate that application of ASCs for wound healing may be influenced by ASC source as well as culture conditions used to expand them. As such, these factors must be taken into consideration before ASCs are used for regenerative purposes. Hindawi 2017 2017-08-23 /pmc/articles/PMC5613713/ /pubmed/29138638 http://dx.doi.org/10.1155/2017/7108458 Text en Copyright © 2017 Lauren H. Mangum et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mangum, Lauren H.
Natesan, Shanmugasundaram
Stone, Randolph
Wrice, Nicole L.
Larson, David A.
Florell, Kyle F.
Christy, Barbara A.
Herzig, Maryanne C.
Cap, Andrew P.
Christy, Robert J.
Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells
title Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells
title_full Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells
title_fullStr Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells
title_full_unstemmed Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells
title_short Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells
title_sort tissue source and cell expansion condition influence phenotypic changes of adipose-derived stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613713/
https://www.ncbi.nlm.nih.gov/pubmed/29138638
http://dx.doi.org/10.1155/2017/7108458
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