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Short-term nicotine exposure induces long-lasting modulation of gustatory plasticity in Caenorhabditis elegans

Nicotine administration induces many effects on animal behavior. In wild-type Caenorhabditis elegans, gustatory plasticity results in reduced chemotaxis toward NaCl of otherwise attractive concentrations after pre-exposure to 100 mM NaCl in the absence of food. However, acute nicotine administration...

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Autores principales: Urushihata, Takuya, Wakabayashi, Tokumitsu, Osato, Shoichi, Yamashita, Tetsuro, Matsuura, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613740/
https://www.ncbi.nlm.nih.gov/pubmed/28955940
http://dx.doi.org/10.1016/j.bbrep.2016.08.008
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author Urushihata, Takuya
Wakabayashi, Tokumitsu
Osato, Shoichi
Yamashita, Tetsuro
Matsuura, Tetsuya
author_facet Urushihata, Takuya
Wakabayashi, Tokumitsu
Osato, Shoichi
Yamashita, Tetsuro
Matsuura, Tetsuya
author_sort Urushihata, Takuya
collection PubMed
description Nicotine administration induces many effects on animal behavior. In wild-type Caenorhabditis elegans, gustatory plasticity results in reduced chemotaxis toward NaCl of otherwise attractive concentrations after pre-exposure to 100 mM NaCl in the absence of food. However, acute nicotine administration during a 15 min pre-exposure period inhibits gustatory plasticity, whereas chronic nicotine administration during worm development facilitates the plasticity. To investigate the relationship between the duration of nicotine administration and its effects, we exposed worms to nicotine for various periods during development. The modulatory effect of nicotine on gustatory plasticity was gradually switched from inhibition to facilitation with increased duration of nicotine administration. Moreover, inhibition of plasticity was sustained after relatively short-term chronic administration, with effects lasting for 45 h after the removal of nicotine. Similar to the acute inhibitory effect after 15 min nicotine pre-exposure, the inhibitory effect after short-term chronic administration was dependent on the nicotinic acetylcholine receptor subunit genes lev-1 and unc-29, and genes involved in serotonin biosynthesis bas-1 and tph-1. The impaired inhibition in bas-1 and tph-1mutants was recovered by exogenous serotonin, demonstrating that serotonin plays an important role in the long-lasting inhibitory effects of short-term chronic nicotine exposure.
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spelling pubmed-56137402017-09-27 Short-term nicotine exposure induces long-lasting modulation of gustatory plasticity in Caenorhabditis elegans Urushihata, Takuya Wakabayashi, Tokumitsu Osato, Shoichi Yamashita, Tetsuro Matsuura, Tetsuya Biochem Biophys Rep Research Article Nicotine administration induces many effects on animal behavior. In wild-type Caenorhabditis elegans, gustatory plasticity results in reduced chemotaxis toward NaCl of otherwise attractive concentrations after pre-exposure to 100 mM NaCl in the absence of food. However, acute nicotine administration during a 15 min pre-exposure period inhibits gustatory plasticity, whereas chronic nicotine administration during worm development facilitates the plasticity. To investigate the relationship between the duration of nicotine administration and its effects, we exposed worms to nicotine for various periods during development. The modulatory effect of nicotine on gustatory plasticity was gradually switched from inhibition to facilitation with increased duration of nicotine administration. Moreover, inhibition of plasticity was sustained after relatively short-term chronic administration, with effects lasting for 45 h after the removal of nicotine. Similar to the acute inhibitory effect after 15 min nicotine pre-exposure, the inhibitory effect after short-term chronic administration was dependent on the nicotinic acetylcholine receptor subunit genes lev-1 and unc-29, and genes involved in serotonin biosynthesis bas-1 and tph-1. The impaired inhibition in bas-1 and tph-1mutants was recovered by exogenous serotonin, demonstrating that serotonin plays an important role in the long-lasting inhibitory effects of short-term chronic nicotine exposure. Elsevier 2016-08-11 /pmc/articles/PMC5613740/ /pubmed/28955940 http://dx.doi.org/10.1016/j.bbrep.2016.08.008 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Urushihata, Takuya
Wakabayashi, Tokumitsu
Osato, Shoichi
Yamashita, Tetsuro
Matsuura, Tetsuya
Short-term nicotine exposure induces long-lasting modulation of gustatory plasticity in Caenorhabditis elegans
title Short-term nicotine exposure induces long-lasting modulation of gustatory plasticity in Caenorhabditis elegans
title_full Short-term nicotine exposure induces long-lasting modulation of gustatory plasticity in Caenorhabditis elegans
title_fullStr Short-term nicotine exposure induces long-lasting modulation of gustatory plasticity in Caenorhabditis elegans
title_full_unstemmed Short-term nicotine exposure induces long-lasting modulation of gustatory plasticity in Caenorhabditis elegans
title_short Short-term nicotine exposure induces long-lasting modulation of gustatory plasticity in Caenorhabditis elegans
title_sort short-term nicotine exposure induces long-lasting modulation of gustatory plasticity in caenorhabditis elegans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613740/
https://www.ncbi.nlm.nih.gov/pubmed/28955940
http://dx.doi.org/10.1016/j.bbrep.2016.08.008
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