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Abundance of RNase4 and RNase5 mRNA and protein in host defence related tissues and secretions in cattle
Members of the RNaseA family are present in various tissues and secretions but their function is not well understood. Some of the RNases are proposed to participate in host defence. RNase4 and RNase5 are present in cows' milk and have antimicrobial activity. However, their presence in many tiss...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613968/ https://www.ncbi.nlm.nih.gov/pubmed/28955965 http://dx.doi.org/10.1016/j.bbrep.2016.09.001 |
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author | Gupta, Sandeep K. Haigh, Brendan J. Wheeler, Thomas T. |
author_facet | Gupta, Sandeep K. Haigh, Brendan J. Wheeler, Thomas T. |
author_sort | Gupta, Sandeep K. |
collection | PubMed |
description | Members of the RNaseA family are present in various tissues and secretions but their function is not well understood. Some of the RNases are proposed to participate in host defence. RNase4 and RNase5 are present in cows' milk and have antimicrobial activity. However, their presence in many tissues and secretions has not been characterised. We hypothesised that these two RNases are present in a range of tissues and secretions where they could contribute to host defence. We therefore, determined the relative abundance of RNase4 and RNase5 mRNA as well as protein levels in a range of host defence related and other tissues as well as a range of secretions in cattle, using real time PCR and western blotting. The two RNases were found to be expressed in liver, lung, pancreas, mammary gland, placenta, endometrium, small intestine, seminal vesicle, salivary gland, kidney, spleen, lymph node, skin as well as testes. Corresponding proteins were also detected in many of the above tissues, as well as in seminal fluid, mammary secretions and saliva. This study provides evidence for the presence of RNase4 and RNase5 in a range of tissues and secretions, as well as some major organs in cattle. The data are consistent with the idea that these proteins could contribute to host defence in these locations. This work contributes to growing body of data suggesting that these proteins contribute to the physiology of the organism in a more complex way than acting merely as digestive enzymes. |
format | Online Article Text |
id | pubmed-5613968 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-56139682017-09-27 Abundance of RNase4 and RNase5 mRNA and protein in host defence related tissues and secretions in cattle Gupta, Sandeep K. Haigh, Brendan J. Wheeler, Thomas T. Biochem Biophys Rep Research Article Members of the RNaseA family are present in various tissues and secretions but their function is not well understood. Some of the RNases are proposed to participate in host defence. RNase4 and RNase5 are present in cows' milk and have antimicrobial activity. However, their presence in many tissues and secretions has not been characterised. We hypothesised that these two RNases are present in a range of tissues and secretions where they could contribute to host defence. We therefore, determined the relative abundance of RNase4 and RNase5 mRNA as well as protein levels in a range of host defence related and other tissues as well as a range of secretions in cattle, using real time PCR and western blotting. The two RNases were found to be expressed in liver, lung, pancreas, mammary gland, placenta, endometrium, small intestine, seminal vesicle, salivary gland, kidney, spleen, lymph node, skin as well as testes. Corresponding proteins were also detected in many of the above tissues, as well as in seminal fluid, mammary secretions and saliva. This study provides evidence for the presence of RNase4 and RNase5 in a range of tissues and secretions, as well as some major organs in cattle. The data are consistent with the idea that these proteins could contribute to host defence in these locations. This work contributes to growing body of data suggesting that these proteins contribute to the physiology of the organism in a more complex way than acting merely as digestive enzymes. Elsevier 2016-09-23 /pmc/articles/PMC5613968/ /pubmed/28955965 http://dx.doi.org/10.1016/j.bbrep.2016.09.001 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Gupta, Sandeep K. Haigh, Brendan J. Wheeler, Thomas T. Abundance of RNase4 and RNase5 mRNA and protein in host defence related tissues and secretions in cattle |
title | Abundance of RNase4 and RNase5 mRNA and protein in host defence related tissues and secretions in cattle |
title_full | Abundance of RNase4 and RNase5 mRNA and protein in host defence related tissues and secretions in cattle |
title_fullStr | Abundance of RNase4 and RNase5 mRNA and protein in host defence related tissues and secretions in cattle |
title_full_unstemmed | Abundance of RNase4 and RNase5 mRNA and protein in host defence related tissues and secretions in cattle |
title_short | Abundance of RNase4 and RNase5 mRNA and protein in host defence related tissues and secretions in cattle |
title_sort | abundance of rnase4 and rnase5 mrna and protein in host defence related tissues and secretions in cattle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613968/ https://www.ncbi.nlm.nih.gov/pubmed/28955965 http://dx.doi.org/10.1016/j.bbrep.2016.09.001 |
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