A high yield optimized method for the production of acylated ACPs enabling the analysis of enzymes involved in P. falciparum fatty acid biosynthesis

The natural substrates of the enzymes involved in type-II fatty acid biosynthesis (FAS-II) are acylated acyl carrier proteins (acyl-ACPs). The state of the art method to produce acyl-ACPs involves the transfer of a phosphopantetheine moiety from CoA to apo-ACP by E. coli holo-ACP synthase (EcACPS),...

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Detalles Bibliográficos
Autores principales: Lauciello, Leonardo, Lack, Gabriela, Scapozza, Leonardo, Perozzo, Remo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613970/
https://www.ncbi.nlm.nih.gov/pubmed/28955970
http://dx.doi.org/10.1016/j.bbrep.2016.09.017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613970/
https://www.ncbi.nlm.nih.gov/pubmed/28955970
http://dx.doi.org/10.1016/j.bbrep.2016.09.017

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