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Distribution of Chlamydia Trachomatis Genotypes in Infective Diseases of the Female Lower Genital Tract
The purpose of this study was to investigate the distribution of Chlamydia trachomatis (CT) genotypes in infective diseases of the female lower genital tract, especially in cervical diseases. This study included 128 CT-positive women. DNA was extracted from cervical swabs. Omp1 gene PCR-RFLP and seq...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614338/ https://www.ncbi.nlm.nih.gov/pubmed/28918430 http://dx.doi.org/10.12659/MSM.902756 |
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author | Chen, Yujie Chen, Jing Yang, Lan Jiang, Yanming Li, Li Yi, Wenjuan Lan, Lifang Zhang, Liuhong |
author_facet | Chen, Yujie Chen, Jing Yang, Lan Jiang, Yanming Li, Li Yi, Wenjuan Lan, Lifang Zhang, Liuhong |
author_sort | Chen, Yujie |
collection | PubMed |
description | The purpose of this study was to investigate the distribution of Chlamydia trachomatis (CT) genotypes in infective diseases of the female lower genital tract, especially in cervical diseases. This study included 128 CT-positive women. DNA was extracted from cervical swabs. Omp1 gene PCR-RFLP and sequencing were used to confirm the subtypes of CT. The association of subtypes with age, clinical symptoms, cervical cytology, and biopsy results was further analyzed. Omp1 gene PCR-RFLP and sequencing showed that the order of prevalent CT genotypes in the female lower genital tract was D (n=38, 29.69%), followed by E (n=28, 21.88%), G (n=21, 16.41%), and F (n=16,12.50%). Genotypes J, H, and K were comparatively rare. Genotype I was not identified in our samples. Further analysis showed that patients with genotype G were more frequently co-infected with other bacteria. Genotype G was also associated with mucopurulent cervicitis (MPC) and cervical intraepithelial neoplasia (CIN). Patients with genotype E were commonly co-infected with HR-HPV. Although genotype D was the most prevalent, it was a relatively low-risk type. These results provide information on distribution of CT genotypes in infective diseases of the female lower genital tract, which is instrumental to developing a vaccine for CT. |
format | Online Article Text |
id | pubmed-5614338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56143382017-10-02 Distribution of Chlamydia Trachomatis Genotypes in Infective Diseases of the Female Lower Genital Tract Chen, Yujie Chen, Jing Yang, Lan Jiang, Yanming Li, Li Yi, Wenjuan Lan, Lifang Zhang, Liuhong Med Sci Monit Hypothesis The purpose of this study was to investigate the distribution of Chlamydia trachomatis (CT) genotypes in infective diseases of the female lower genital tract, especially in cervical diseases. This study included 128 CT-positive women. DNA was extracted from cervical swabs. Omp1 gene PCR-RFLP and sequencing were used to confirm the subtypes of CT. The association of subtypes with age, clinical symptoms, cervical cytology, and biopsy results was further analyzed. Omp1 gene PCR-RFLP and sequencing showed that the order of prevalent CT genotypes in the female lower genital tract was D (n=38, 29.69%), followed by E (n=28, 21.88%), G (n=21, 16.41%), and F (n=16,12.50%). Genotypes J, H, and K were comparatively rare. Genotype I was not identified in our samples. Further analysis showed that patients with genotype G were more frequently co-infected with other bacteria. Genotype G was also associated with mucopurulent cervicitis (MPC) and cervical intraepithelial neoplasia (CIN). Patients with genotype E were commonly co-infected with HR-HPV. Although genotype D was the most prevalent, it was a relatively low-risk type. These results provide information on distribution of CT genotypes in infective diseases of the female lower genital tract, which is instrumental to developing a vaccine for CT. International Scientific Literature, Inc. 2017-09-17 /pmc/articles/PMC5614338/ /pubmed/28918430 http://dx.doi.org/10.12659/MSM.902756 Text en © Med Sci Monit, 2017 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Hypothesis Chen, Yujie Chen, Jing Yang, Lan Jiang, Yanming Li, Li Yi, Wenjuan Lan, Lifang Zhang, Liuhong Distribution of Chlamydia Trachomatis Genotypes in Infective Diseases of the Female Lower Genital Tract |
title | Distribution of Chlamydia Trachomatis Genotypes in Infective Diseases of the Female Lower Genital Tract |
title_full | Distribution of Chlamydia Trachomatis Genotypes in Infective Diseases of the Female Lower Genital Tract |
title_fullStr | Distribution of Chlamydia Trachomatis Genotypes in Infective Diseases of the Female Lower Genital Tract |
title_full_unstemmed | Distribution of Chlamydia Trachomatis Genotypes in Infective Diseases of the Female Lower Genital Tract |
title_short | Distribution of Chlamydia Trachomatis Genotypes in Infective Diseases of the Female Lower Genital Tract |
title_sort | distribution of chlamydia trachomatis genotypes in infective diseases of the female lower genital tract |
topic | Hypothesis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614338/ https://www.ncbi.nlm.nih.gov/pubmed/28918430 http://dx.doi.org/10.12659/MSM.902756 |
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