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Ancient permafrost staphylococci carry antibiotic resistance genes
Background: Permafrost preserves a variety of viable ancient microorganisms. Some of them can be cultivated after being kept at subzero temperatures for thousands or even millions of years. Objective: To cultivate bacterial strains from permafrost. Design: We isolated and cultivated two bacterial st...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614383/ https://www.ncbi.nlm.nih.gov/pubmed/28959177 http://dx.doi.org/10.1080/16512235.2017.1345574 |
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author | Kashuba, Elena Dmitriev, Alexey A. Kamal, Shady Mansour Melefors, Ojar Griva, Gennady Römling, Ute Ernberg, Ingemar Kashuba, Vladimir Brouchkov, Anatoli |
author_facet | Kashuba, Elena Dmitriev, Alexey A. Kamal, Shady Mansour Melefors, Ojar Griva, Gennady Römling, Ute Ernberg, Ingemar Kashuba, Vladimir Brouchkov, Anatoli |
author_sort | Kashuba, Elena |
collection | PubMed |
description | Background: Permafrost preserves a variety of viable ancient microorganisms. Some of them can be cultivated after being kept at subzero temperatures for thousands or even millions of years. Objective: To cultivate bacterial strains from permafrost. Design: We isolated and cultivated two bacterial strains from permafrost that was obtained at Mammoth Mountain in Siberia and attributed to the Middle Miocene. Bacterial genomic DNA was sequenced with 40–60× coverage and high-quality contigs were assembled. The first strain was assigned to Staphylococcus warneri species (designated MMP1) and the second one to Staphylococcus hominis species (designated MMP2), based on the classification of 16S ribosomal RNA genes and genomic sequences. Results: Genomic sequence analysis revealed the close relation of the isolated ancient bacteria to the modern bacteria of this species. Moreover, several genes associated with resistance to different groups of antibiotics were found in the S. hominis MMP2 genome. Conclusions: These findings supports a hypothesis that antibiotic resistance has an ancient origin. The enrichment of cultivated bacterial communities with ancient permafrost strains is essential for the analysis of bacterial evolution and antibiotic resistance. |
format | Online Article Text |
id | pubmed-5614383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-56143832017-09-28 Ancient permafrost staphylococci carry antibiotic resistance genes Kashuba, Elena Dmitriev, Alexey A. Kamal, Shady Mansour Melefors, Ojar Griva, Gennady Römling, Ute Ernberg, Ingemar Kashuba, Vladimir Brouchkov, Anatoli Microb Ecol Health Dis Original Article Background: Permafrost preserves a variety of viable ancient microorganisms. Some of them can be cultivated after being kept at subzero temperatures for thousands or even millions of years. Objective: To cultivate bacterial strains from permafrost. Design: We isolated and cultivated two bacterial strains from permafrost that was obtained at Mammoth Mountain in Siberia and attributed to the Middle Miocene. Bacterial genomic DNA was sequenced with 40–60× coverage and high-quality contigs were assembled. The first strain was assigned to Staphylococcus warneri species (designated MMP1) and the second one to Staphylococcus hominis species (designated MMP2), based on the classification of 16S ribosomal RNA genes and genomic sequences. Results: Genomic sequence analysis revealed the close relation of the isolated ancient bacteria to the modern bacteria of this species. Moreover, several genes associated with resistance to different groups of antibiotics were found in the S. hominis MMP2 genome. Conclusions: These findings supports a hypothesis that antibiotic resistance has an ancient origin. The enrichment of cultivated bacterial communities with ancient permafrost strains is essential for the analysis of bacterial evolution and antibiotic resistance. Taylor & Francis 2017-01-01 /pmc/articles/PMC5614383/ /pubmed/28959177 http://dx.doi.org/10.1080/16512235.2017.1345574 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kashuba, Elena Dmitriev, Alexey A. Kamal, Shady Mansour Melefors, Ojar Griva, Gennady Römling, Ute Ernberg, Ingemar Kashuba, Vladimir Brouchkov, Anatoli Ancient permafrost staphylococci carry antibiotic resistance genes |
title | Ancient permafrost staphylococci carry antibiotic resistance genes |
title_full | Ancient permafrost staphylococci carry antibiotic resistance genes |
title_fullStr | Ancient permafrost staphylococci carry antibiotic resistance genes |
title_full_unstemmed | Ancient permafrost staphylococci carry antibiotic resistance genes |
title_short | Ancient permafrost staphylococci carry antibiotic resistance genes |
title_sort | ancient permafrost staphylococci carry antibiotic resistance genes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614383/ https://www.ncbi.nlm.nih.gov/pubmed/28959177 http://dx.doi.org/10.1080/16512235.2017.1345574 |
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