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Effects of isoleucine 135 side chain length on the cofactor donor-acceptor distance within F(420)H(2):NADP(+) oxidoreductase: A kinetic analysis()
F(420)H(2):NADP(+) Oxidoreductase (Fno) catalyzes the reversible reduction of NADP(+) to NADPH by transferring a hydride from the reduced F(420) cofactor. Here, we have employed binding studies, steady-state and pre steady-state kinetic methods upon wtFno and isoleucine 135 (I135) Fno variants in or...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614548/ https://www.ncbi.nlm.nih.gov/pubmed/28955995 http://dx.doi.org/10.1016/j.bbrep.2016.11.012 |