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Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain

This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type I...

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Autores principales: Morita, Makoto, Sugihara, Hidemitsu, Tokunaka, Kazuhiro, Tomura, Arihiro, Saiga, Kan, Sato, Takamichi, Imamura, Yasutada, Hayashi, Toshihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614550/
https://www.ncbi.nlm.nih.gov/pubmed/28955997
http://dx.doi.org/10.1016/j.bbrep.2016.11.013
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author Morita, Makoto
Sugihara, Hidemitsu
Tokunaka, Kazuhiro
Tomura, Arihiro
Saiga, Kan
Sato, Takamichi
Imamura, Yasutada
Hayashi, Toshihiko
author_facet Morita, Makoto
Sugihara, Hidemitsu
Tokunaka, Kazuhiro
Tomura, Arihiro
Saiga, Kan
Sato, Takamichi
Imamura, Yasutada
Hayashi, Toshihiko
author_sort Morita, Makoto
collection PubMed
description This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type IV collagen in the triple-helical conformation. Three antibodies, #141, #179 and #370, with different epitopes in NTH α1(IV) were found to be reactive with the nascent polypeptide secreted from human normal cells and a human carcinoma cell line. The antibodies with different epitopes may provide a key method for elucidating the physiological function and tissue distribution of NTH α1(IV), which is distinct from the chain derived from triple-helical type IV collagen.
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spelling pubmed-56145502017-09-27 Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain Morita, Makoto Sugihara, Hidemitsu Tokunaka, Kazuhiro Tomura, Arihiro Saiga, Kan Sato, Takamichi Imamura, Yasutada Hayashi, Toshihiko Biochem Biophys Rep Research Article This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type IV collagen in the triple-helical conformation. Three antibodies, #141, #179 and #370, with different epitopes in NTH α1(IV) were found to be reactive with the nascent polypeptide secreted from human normal cells and a human carcinoma cell line. The antibodies with different epitopes may provide a key method for elucidating the physiological function and tissue distribution of NTH α1(IV), which is distinct from the chain derived from triple-helical type IV collagen. Elsevier 2016-12-08 /pmc/articles/PMC5614550/ /pubmed/28955997 http://dx.doi.org/10.1016/j.bbrep.2016.11.013 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Morita, Makoto
Sugihara, Hidemitsu
Tokunaka, Kazuhiro
Tomura, Arihiro
Saiga, Kan
Sato, Takamichi
Imamura, Yasutada
Hayashi, Toshihiko
Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain
title Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain
title_full Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain
title_fullStr Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain
title_full_unstemmed Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain
title_short Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain
title_sort preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type iv collagen alpha 1 chain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614550/
https://www.ncbi.nlm.nih.gov/pubmed/28955997
http://dx.doi.org/10.1016/j.bbrep.2016.11.013
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