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Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain
This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type I...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614550/ https://www.ncbi.nlm.nih.gov/pubmed/28955997 http://dx.doi.org/10.1016/j.bbrep.2016.11.013 |
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author | Morita, Makoto Sugihara, Hidemitsu Tokunaka, Kazuhiro Tomura, Arihiro Saiga, Kan Sato, Takamichi Imamura, Yasutada Hayashi, Toshihiko |
author_facet | Morita, Makoto Sugihara, Hidemitsu Tokunaka, Kazuhiro Tomura, Arihiro Saiga, Kan Sato, Takamichi Imamura, Yasutada Hayashi, Toshihiko |
author_sort | Morita, Makoto |
collection | PubMed |
description | This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type IV collagen in the triple-helical conformation. Three antibodies, #141, #179 and #370, with different epitopes in NTH α1(IV) were found to be reactive with the nascent polypeptide secreted from human normal cells and a human carcinoma cell line. The antibodies with different epitopes may provide a key method for elucidating the physiological function and tissue distribution of NTH α1(IV), which is distinct from the chain derived from triple-helical type IV collagen. |
format | Online Article Text |
id | pubmed-5614550 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-56145502017-09-27 Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain Morita, Makoto Sugihara, Hidemitsu Tokunaka, Kazuhiro Tomura, Arihiro Saiga, Kan Sato, Takamichi Imamura, Yasutada Hayashi, Toshihiko Biochem Biophys Rep Research Article This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type IV collagen in the triple-helical conformation. Three antibodies, #141, #179 and #370, with different epitopes in NTH α1(IV) were found to be reactive with the nascent polypeptide secreted from human normal cells and a human carcinoma cell line. The antibodies with different epitopes may provide a key method for elucidating the physiological function and tissue distribution of NTH α1(IV), which is distinct from the chain derived from triple-helical type IV collagen. Elsevier 2016-12-08 /pmc/articles/PMC5614550/ /pubmed/28955997 http://dx.doi.org/10.1016/j.bbrep.2016.11.013 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Morita, Makoto Sugihara, Hidemitsu Tokunaka, Kazuhiro Tomura, Arihiro Saiga, Kan Sato, Takamichi Imamura, Yasutada Hayashi, Toshihiko Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain |
title | Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain |
title_full | Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain |
title_fullStr | Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain |
title_full_unstemmed | Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain |
title_short | Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain |
title_sort | preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type iv collagen alpha 1 chain |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614550/ https://www.ncbi.nlm.nih.gov/pubmed/28955997 http://dx.doi.org/10.1016/j.bbrep.2016.11.013 |
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