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Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle

Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV chall...

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Autores principales: Soria, Ivana, Quattrocchi, Valeria, Langellotti, Cecilia, Gammella, Mariela, Digiacomo, Sebastian, Garcia de la Torre, Beatriz, Andreu, David, Montoya, Maria, Sobrino, Francisco, Blanco, Esther, Zamorano, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614567/
https://www.ncbi.nlm.nih.gov/pubmed/28949998
http://dx.doi.org/10.1371/journal.pone.0185184
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author Soria, Ivana
Quattrocchi, Valeria
Langellotti, Cecilia
Gammella, Mariela
Digiacomo, Sebastian
Garcia de la Torre, Beatriz
Andreu, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia
author_facet Soria, Ivana
Quattrocchi, Valeria
Langellotti, Cecilia
Gammella, Mariela
Digiacomo, Sebastian
Garcia de la Torre, Beatriz
Andreu, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia
author_sort Soria, Ivana
collection PubMed
description Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B(2)T and B(4)T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B(2)T or B(4)T were protected to podal generalization. Moreover, bovines immunized with B(4)T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B(2)T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B(2)T or B(4)T groups respectively, showed anti-FMDV IgA titers. In addition, B(2)T and B(4)T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B(2)T or B(4)T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.
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spelling pubmed-56145672017-10-09 Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle Soria, Ivana Quattrocchi, Valeria Langellotti, Cecilia Gammella, Mariela Digiacomo, Sebastian Garcia de la Torre, Beatriz Andreu, David Montoya, Maria Sobrino, Francisco Blanco, Esther Zamorano, Patricia PLoS One Research Article Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B(2)T and B(4)T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B(2)T or B(4)T were protected to podal generalization. Moreover, bovines immunized with B(4)T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B(2)T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B(2)T or B(4)T groups respectively, showed anti-FMDV IgA titers. In addition, B(2)T and B(4)T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B(2)T or B(4)T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential. Public Library of Science 2017-09-26 /pmc/articles/PMC5614567/ /pubmed/28949998 http://dx.doi.org/10.1371/journal.pone.0185184 Text en © 2017 Soria et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Soria, Ivana
Quattrocchi, Valeria
Langellotti, Cecilia
Gammella, Mariela
Digiacomo, Sebastian
Garcia de la Torre, Beatriz
Andreu, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia
Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_full Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_fullStr Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_full_unstemmed Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_short Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_sort dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614567/
https://www.ncbi.nlm.nih.gov/pubmed/28949998
http://dx.doi.org/10.1371/journal.pone.0185184
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