Isolation and partial characterization of collagen from outer skin of Sepia pharaonis (Ehrenberg, 1831) from Puducherry coast

Type I collagen from outer skin of Sepia pharaonis was extracted and partially characterized. Yield of Acid Soluble Collagen (ASC) and Pepsin Soluble Collagen (PSC) were calculated as 1.66% and 3.93% and the total protein content of ASC and PSC were found as 18.4% and 48.6%. FT-IR spectrum of ASC an...

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Autores principales: Krishnamoorthi, Jayalakshmi, Ramasamy, Pasiyappazham, Shanmugam, Vairamani, Shanmugam, Annaian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614650/
https://www.ncbi.nlm.nih.gov/pubmed/28955735
http://dx.doi.org/10.1016/j.bbrep.2017.02.006
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author Krishnamoorthi, Jayalakshmi
Ramasamy, Pasiyappazham
Shanmugam, Vairamani
Shanmugam, Annaian
author_facet Krishnamoorthi, Jayalakshmi
Ramasamy, Pasiyappazham
Shanmugam, Vairamani
Shanmugam, Annaian
author_sort Krishnamoorthi, Jayalakshmi
collection PubMed
description Type I collagen from outer skin of Sepia pharaonis was extracted and partially characterized. Yield of Acid Soluble Collagen (ASC) and Pepsin Soluble Collagen (PSC) were calculated as 1.66% and 3.93% and the total protein content of ASC and PSC were found as 18.4% and 48.6%. FT-IR spectrum of ASC and PSC recorded 12 and 14 peaks, respectively. (1)H NMR spectrum of ASC showed singlets at 1.23 ppm, 3.1 ppm, 3.55 ppm and 3.7 ppm and PSC at 1.23 ppm and 2.08 ppm. The molecular weight for ASC was calculated as 102 kDa and for PSC as 110, 108 and 102 kDa through SDS-PAGE. Differential Scanning Calorimetry (DSC) results supported that PSC withstand high thermal stability (82.85 °C) than ASC (73.13 °C). Higher denaturation temperature with high molecular weight well support the property of type I collagen from skin of S. pharaonis and it could be used as another potent source for the extraction of collagen.
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spelling pubmed-56146502017-09-27 Isolation and partial characterization of collagen from outer skin of Sepia pharaonis (Ehrenberg, 1831) from Puducherry coast Krishnamoorthi, Jayalakshmi Ramasamy, Pasiyappazham Shanmugam, Vairamani Shanmugam, Annaian Biochem Biophys Rep Research Article Type I collagen from outer skin of Sepia pharaonis was extracted and partially characterized. Yield of Acid Soluble Collagen (ASC) and Pepsin Soluble Collagen (PSC) were calculated as 1.66% and 3.93% and the total protein content of ASC and PSC were found as 18.4% and 48.6%. FT-IR spectrum of ASC and PSC recorded 12 and 14 peaks, respectively. (1)H NMR spectrum of ASC showed singlets at 1.23 ppm, 3.1 ppm, 3.55 ppm and 3.7 ppm and PSC at 1.23 ppm and 2.08 ppm. The molecular weight for ASC was calculated as 102 kDa and for PSC as 110, 108 and 102 kDa through SDS-PAGE. Differential Scanning Calorimetry (DSC) results supported that PSC withstand high thermal stability (82.85 °C) than ASC (73.13 °C). Higher denaturation temperature with high molecular weight well support the property of type I collagen from skin of S. pharaonis and it could be used as another potent source for the extraction of collagen. Elsevier 2017-02-27 /pmc/articles/PMC5614650/ /pubmed/28955735 http://dx.doi.org/10.1016/j.bbrep.2017.02.006 Text en © 2017 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Krishnamoorthi, Jayalakshmi
Ramasamy, Pasiyappazham
Shanmugam, Vairamani
Shanmugam, Annaian
Isolation and partial characterization of collagen from outer skin of Sepia pharaonis (Ehrenberg, 1831) from Puducherry coast
title Isolation and partial characterization of collagen from outer skin of Sepia pharaonis (Ehrenberg, 1831) from Puducherry coast
title_full Isolation and partial characterization of collagen from outer skin of Sepia pharaonis (Ehrenberg, 1831) from Puducherry coast
title_fullStr Isolation and partial characterization of collagen from outer skin of Sepia pharaonis (Ehrenberg, 1831) from Puducherry coast
title_full_unstemmed Isolation and partial characterization of collagen from outer skin of Sepia pharaonis (Ehrenberg, 1831) from Puducherry coast
title_short Isolation and partial characterization of collagen from outer skin of Sepia pharaonis (Ehrenberg, 1831) from Puducherry coast
title_sort isolation and partial characterization of collagen from outer skin of sepia pharaonis (ehrenberg, 1831) from puducherry coast
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614650/
https://www.ncbi.nlm.nih.gov/pubmed/28955735
http://dx.doi.org/10.1016/j.bbrep.2017.02.006
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