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Synthesis and characterization of pHLIP(®) coated gold nanoparticles

Novel approaches in synthesis of spherical and multispiked gold nanoparticles coated with polyethylene glycol (PEG) and pH Low Insertion Peptide (pHLIP(®)) were introduced. The presence of a tumor-targeting pHLIP(®) peptide in the nanoparticle coating enhances the stability of particles in solution...

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Autores principales: Daniels, Jennifer L., Crawford, Troy M., Andreev, Oleg A., Reshetnyak, Yana K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614664/
https://www.ncbi.nlm.nih.gov/pubmed/28955736
http://dx.doi.org/10.1016/j.bbrep.2017.02.008
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author Daniels, Jennifer L.
Crawford, Troy M.
Andreev, Oleg A.
Reshetnyak, Yana K.
author_facet Daniels, Jennifer L.
Crawford, Troy M.
Andreev, Oleg A.
Reshetnyak, Yana K.
author_sort Daniels, Jennifer L.
collection PubMed
description Novel approaches in synthesis of spherical and multispiked gold nanoparticles coated with polyethylene glycol (PEG) and pH Low Insertion Peptide (pHLIP(®)) were introduced. The presence of a tumor-targeting pHLIP(®) peptide in the nanoparticle coating enhances the stability of particles in solution and promotes a pH-dependent cellular uptake. The spherical particles were prepared with sodium citrate as a gold reducing agent to form particles of 7.0±2.5 nm in mean metallic core diameter and ∼43 nm in mean hydrodynamic diameter. The particles that were injected into tumors in mice (21 µg of gold) were homogeneously distributed within a tumor mass with no staining of the muscle tissue adjacent to the tumor. Up to 30% of the injected gold dose remained within the tumor one hour post-injection. The multispiked gold nanoparticles with a mean metallic core diameter of 146.0±50.4 nm and a mean hydrodynamic size of ~161 nm were prepared using ascorbic acid as a reducing agent and disk-like bicelles as a template. Only the presence of a soft template, like bicelles, ensured the appearance of spiked nanoparticles with resonance in the near infrared region. The irradiation of spiked gold nanoparticles by an 805 nm laser led to the time- and concentration-dependent increase of temperature. Both pHLIP(®) and PEG coated gold spherical and multispiked nanoparticles might find application in radiation and thermal therapies of tumors.
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spelling pubmed-56146642017-09-27 Synthesis and characterization of pHLIP(®) coated gold nanoparticles Daniels, Jennifer L. Crawford, Troy M. Andreev, Oleg A. Reshetnyak, Yana K. Biochem Biophys Rep Research Article Novel approaches in synthesis of spherical and multispiked gold nanoparticles coated with polyethylene glycol (PEG) and pH Low Insertion Peptide (pHLIP(®)) were introduced. The presence of a tumor-targeting pHLIP(®) peptide in the nanoparticle coating enhances the stability of particles in solution and promotes a pH-dependent cellular uptake. The spherical particles were prepared with sodium citrate as a gold reducing agent to form particles of 7.0±2.5 nm in mean metallic core diameter and ∼43 nm in mean hydrodynamic diameter. The particles that were injected into tumors in mice (21 µg of gold) were homogeneously distributed within a tumor mass with no staining of the muscle tissue adjacent to the tumor. Up to 30% of the injected gold dose remained within the tumor one hour post-injection. The multispiked gold nanoparticles with a mean metallic core diameter of 146.0±50.4 nm and a mean hydrodynamic size of ~161 nm were prepared using ascorbic acid as a reducing agent and disk-like bicelles as a template. Only the presence of a soft template, like bicelles, ensured the appearance of spiked nanoparticles with resonance in the near infrared region. The irradiation of spiked gold nanoparticles by an 805 nm laser led to the time- and concentration-dependent increase of temperature. Both pHLIP(®) and PEG coated gold spherical and multispiked nanoparticles might find application in radiation and thermal therapies of tumors. Elsevier 2017-02-28 /pmc/articles/PMC5614664/ /pubmed/28955736 http://dx.doi.org/10.1016/j.bbrep.2017.02.008 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Daniels, Jennifer L.
Crawford, Troy M.
Andreev, Oleg A.
Reshetnyak, Yana K.
Synthesis and characterization of pHLIP(®) coated gold nanoparticles
title Synthesis and characterization of pHLIP(®) coated gold nanoparticles
title_full Synthesis and characterization of pHLIP(®) coated gold nanoparticles
title_fullStr Synthesis and characterization of pHLIP(®) coated gold nanoparticles
title_full_unstemmed Synthesis and characterization of pHLIP(®) coated gold nanoparticles
title_short Synthesis and characterization of pHLIP(®) coated gold nanoparticles
title_sort synthesis and characterization of phlip(®) coated gold nanoparticles
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614664/
https://www.ncbi.nlm.nih.gov/pubmed/28955736
http://dx.doi.org/10.1016/j.bbrep.2017.02.008
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