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The para isomer of dinitrobenzene disrupts redox homeostasis in liver and kidney of male wistar rats

BACKGROUND: Para-Dinitrobenzene (p-DNB) is one of the isomers of dinitrobenzene which have been detected as environmental toxicants. Skin irritation and organ toxicities are likely for industrial workers exposed to p-DNB. This study evaluated the effect of sub-chronic exposure of rats to p-DNB on ce...

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Autores principales: Sangodele, Janet Olayemi, Olaleye, Mary Tolulope, Monsees, Thomas K., Akinmoladun, Afolabi Clement
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614678/
https://www.ncbi.nlm.nih.gov/pubmed/28955757
http://dx.doi.org/10.1016/j.bbrep.2017.04.017
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author Sangodele, Janet Olayemi
Olaleye, Mary Tolulope
Monsees, Thomas K.
Akinmoladun, Afolabi Clement
author_facet Sangodele, Janet Olayemi
Olaleye, Mary Tolulope
Monsees, Thomas K.
Akinmoladun, Afolabi Clement
author_sort Sangodele, Janet Olayemi
collection PubMed
description BACKGROUND: Para-Dinitrobenzene (p-DNB) is one of the isomers of dinitrobenzene which have been detected as environmental toxicants. Skin irritation and organ toxicities are likely for industrial workers exposed to p-DNB. This study evaluated the effect of sub-chronic exposure of rats to p-DNB on cellular redox balance, hepatic and renal integrity. METHODS: Forty eight male Wistar rats weighing 160–180 g were administered 50, 75, 1000 and 2000 mg/kg b.wt (body weight) of p-DNB or an equivalent volume of vehicle (control) orally and topically for 14 days. After the period of treatment, the activities of kidney and liver catalase (CAT), alkaline phosphatase (ALP) and superoxide dismutase (SOD) as well as extent of renal and hepatic lipid peroxidation (LPO) were determined. Serum ALP activity and plasma urea concentration were also evaluated. RESULTS: Compared with control animals, p-DNB -administered rats showed decrease in the body and relative kidney and liver weights as well as increased renal and hepatic hydrogen peroxide and lipid peroxidation levels accompanied by decreased superoxide dismutase and catalase activities. However, p-DNB caused a significant increase in plasma urea concentration and serum, liver and kidney ALP activities relative to control. In addition, p-DNB caused periportal infiltration, severe macro vesicular steatosis and hepatic necrosis in the liver. CONCLUSIONS: Our findings show that sub-chronic oral and sub-dermal administration of p-DNB may produce hepato-nephrotoxicity through oxidative stress.
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spelling pubmed-56146782017-09-27 The para isomer of dinitrobenzene disrupts redox homeostasis in liver and kidney of male wistar rats Sangodele, Janet Olayemi Olaleye, Mary Tolulope Monsees, Thomas K. Akinmoladun, Afolabi Clement Biochem Biophys Rep Research Article BACKGROUND: Para-Dinitrobenzene (p-DNB) is one of the isomers of dinitrobenzene which have been detected as environmental toxicants. Skin irritation and organ toxicities are likely for industrial workers exposed to p-DNB. This study evaluated the effect of sub-chronic exposure of rats to p-DNB on cellular redox balance, hepatic and renal integrity. METHODS: Forty eight male Wistar rats weighing 160–180 g were administered 50, 75, 1000 and 2000 mg/kg b.wt (body weight) of p-DNB or an equivalent volume of vehicle (control) orally and topically for 14 days. After the period of treatment, the activities of kidney and liver catalase (CAT), alkaline phosphatase (ALP) and superoxide dismutase (SOD) as well as extent of renal and hepatic lipid peroxidation (LPO) were determined. Serum ALP activity and plasma urea concentration were also evaluated. RESULTS: Compared with control animals, p-DNB -administered rats showed decrease in the body and relative kidney and liver weights as well as increased renal and hepatic hydrogen peroxide and lipid peroxidation levels accompanied by decreased superoxide dismutase and catalase activities. However, p-DNB caused a significant increase in plasma urea concentration and serum, liver and kidney ALP activities relative to control. In addition, p-DNB caused periportal infiltration, severe macro vesicular steatosis and hepatic necrosis in the liver. CONCLUSIONS: Our findings show that sub-chronic oral and sub-dermal administration of p-DNB may produce hepato-nephrotoxicity through oxidative stress. Elsevier 2017-05-04 /pmc/articles/PMC5614678/ /pubmed/28955757 http://dx.doi.org/10.1016/j.bbrep.2017.04.017 Text en © 2017 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Sangodele, Janet Olayemi
Olaleye, Mary Tolulope
Monsees, Thomas K.
Akinmoladun, Afolabi Clement
The para isomer of dinitrobenzene disrupts redox homeostasis in liver and kidney of male wistar rats
title The para isomer of dinitrobenzene disrupts redox homeostasis in liver and kidney of male wistar rats
title_full The para isomer of dinitrobenzene disrupts redox homeostasis in liver and kidney of male wistar rats
title_fullStr The para isomer of dinitrobenzene disrupts redox homeostasis in liver and kidney of male wistar rats
title_full_unstemmed The para isomer of dinitrobenzene disrupts redox homeostasis in liver and kidney of male wistar rats
title_short The para isomer of dinitrobenzene disrupts redox homeostasis in liver and kidney of male wistar rats
title_sort para isomer of dinitrobenzene disrupts redox homeostasis in liver and kidney of male wistar rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614678/
https://www.ncbi.nlm.nih.gov/pubmed/28955757
http://dx.doi.org/10.1016/j.bbrep.2017.04.017
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