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The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization

A high throughput screening (HTS) methodology for evaluation of cellular lipid content based on Nile red fluorescence reads using black background 96-wells test plates and a plate reader equipment allowed the rapid intracellular lipid estimation of strains from a Brazilian phylloplane yeast collecti...

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Autores principales: Ramírez-Castrillón, Mauricio, Jaramillo-Garcia, Victoria P., Rosa, Priscila D., Landell, Melissa F., Vu, Duong, Fabricio, Mariana F., Ayub, Marco A. Z., Robert, Vincent, Henriques, João A. P., Valente, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614974/
https://www.ncbi.nlm.nih.gov/pubmed/29018411
http://dx.doi.org/10.3389/fmicb.2017.01776
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author Ramírez-Castrillón, Mauricio
Jaramillo-Garcia, Victoria P.
Rosa, Priscila D.
Landell, Melissa F.
Vu, Duong
Fabricio, Mariana F.
Ayub, Marco A. Z.
Robert, Vincent
Henriques, João A. P.
Valente, Patricia
author_facet Ramírez-Castrillón, Mauricio
Jaramillo-Garcia, Victoria P.
Rosa, Priscila D.
Landell, Melissa F.
Vu, Duong
Fabricio, Mariana F.
Ayub, Marco A. Z.
Robert, Vincent
Henriques, João A. P.
Valente, Patricia
author_sort Ramírez-Castrillón, Mauricio
collection PubMed
description A high throughput screening (HTS) methodology for evaluation of cellular lipid content based on Nile red fluorescence reads using black background 96-wells test plates and a plate reader equipment allowed the rapid intracellular lipid estimation of strains from a Brazilian phylloplane yeast collection. A new oleaginous yeast, Meyerozyma guilliermondii BI281A, was selected, for which the gravimetric determination of total lipids relative to dry weight was 52.38% for glucose or 34.97% for pure glycerol. The lipid production was optimized obtaining 108 mg/L of neutral lipids using pure glycerol as carbon source, and the strain proved capable of accumulating oil using raw glycerol from a biodiesel refinery. The lipid profile showed monounsaturated fatty acids (MUFA) varying between 56 or 74% in pure or raw glycerol, respectively. M. guilliermondii BI281A bears potential as a new biodiesel feedstock.
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spelling pubmed-56149742017-10-10 The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization Ramírez-Castrillón, Mauricio Jaramillo-Garcia, Victoria P. Rosa, Priscila D. Landell, Melissa F. Vu, Duong Fabricio, Mariana F. Ayub, Marco A. Z. Robert, Vincent Henriques, João A. P. Valente, Patricia Front Microbiol Microbiology A high throughput screening (HTS) methodology for evaluation of cellular lipid content based on Nile red fluorescence reads using black background 96-wells test plates and a plate reader equipment allowed the rapid intracellular lipid estimation of strains from a Brazilian phylloplane yeast collection. A new oleaginous yeast, Meyerozyma guilliermondii BI281A, was selected, for which the gravimetric determination of total lipids relative to dry weight was 52.38% for glucose or 34.97% for pure glycerol. The lipid production was optimized obtaining 108 mg/L of neutral lipids using pure glycerol as carbon source, and the strain proved capable of accumulating oil using raw glycerol from a biodiesel refinery. The lipid profile showed monounsaturated fatty acids (MUFA) varying between 56 or 74% in pure or raw glycerol, respectively. M. guilliermondii BI281A bears potential as a new biodiesel feedstock. Frontiers Media S.A. 2017-09-22 /pmc/articles/PMC5614974/ /pubmed/29018411 http://dx.doi.org/10.3389/fmicb.2017.01776 Text en Copyright © 2017 Ramírez-Castrillón, Jaramillo-Garcia, Rosa, Landell, Vu, Fabricio, Ayub, Robert, Henriques and Valente. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ramírez-Castrillón, Mauricio
Jaramillo-Garcia, Victoria P.
Rosa, Priscila D.
Landell, Melissa F.
Vu, Duong
Fabricio, Mariana F.
Ayub, Marco A. Z.
Robert, Vincent
Henriques, João A. P.
Valente, Patricia
The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization
title The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization
title_full The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization
title_fullStr The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization
title_full_unstemmed The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization
title_short The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization
title_sort oleaginous yeast meyerozyma guilliermondii bi281a as a new potential biodiesel feedstock: selection and lipid production optimization
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5614974/
https://www.ncbi.nlm.nih.gov/pubmed/29018411
http://dx.doi.org/10.3389/fmicb.2017.01776
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