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Efficient engineering of chromosomal ribosome binding site libraries in mismatch repair proficient Escherichia coli

Multiplexed gene expression optimization via modulation of gene translation efficiency through ribosome binding site (RBS) engineering is a valuable approach for optimizing artificial properties in bacteria, ranging from genetic circuits to production pathways. Established algorithms design smart RB...

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Detalles Bibliográficos
Autores principales: Oesterle, Sabine, Gerngross, Daniel, Schmitt, Steven, Roberts, Tania Michelle, Panke, Sven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615074/
https://www.ncbi.nlm.nih.gov/pubmed/28951570
http://dx.doi.org/10.1038/s41598-017-12395-3