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Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep

The aim of the present study was to investigate the effects of livestock feed supplemented with grape pomace (GP) or olive oil mill wastewater (OMW) byproducts on the enzymatic activity and protein expression of antioxidants enzymes, in liver and spleen tissue of sheep. Thus, 36 male sheep of Chios...

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Autores principales: Kerasioti, Efthalia, Terzopoulou, Zoi, Komini, Ourania, Kafantaris, Ioannis, Makri, Sotiria, Stagos, Dimitrios, Gerasopoulos, Konstantinos, Anisimov, Nikita Y., Tsatsakis, Aristides M., Kouretas, Demetrios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615154/
https://www.ncbi.nlm.nih.gov/pubmed/28959661
http://dx.doi.org/10.1016/j.toxrep.2017.06.007
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author Kerasioti, Efthalia
Terzopoulou, Zoi
Komini, Ourania
Kafantaris, Ioannis
Makri, Sotiria
Stagos, Dimitrios
Gerasopoulos, Konstantinos
Anisimov, Nikita Y.
Tsatsakis, Aristides M.
Kouretas, Demetrios
author_facet Kerasioti, Efthalia
Terzopoulou, Zoi
Komini, Ourania
Kafantaris, Ioannis
Makri, Sotiria
Stagos, Dimitrios
Gerasopoulos, Konstantinos
Anisimov, Nikita Y.
Tsatsakis, Aristides M.
Kouretas, Demetrios
author_sort Kerasioti, Efthalia
collection PubMed
description The aim of the present study was to investigate the effects of livestock feed supplemented with grape pomace (GP) or olive oil mill wastewater (OMW) byproducts on the enzymatic activity and protein expression of antioxidants enzymes, in liver and spleen tissue of sheep. Thus, 36 male sheep of Chios breed were divided into 3 homogeneous groups, control group (n = 12), GP group (n = 12) and OMW group (n = 12), receiving standard or experimental feed. Liver and spleen tissues were collected at 42 and 70 days post-birth. The enzymatic activity of superoxide dismutase (SOD) and glutathione-s-transferase (GST) and also the protein expression of γ-synthase glutamyl custeine (γ-GCS) were determined in these tissues. The results showed GP group exhibited increased enzymatic activity of GST and protein expression of γ-GCS in liver compared to control group. In GP group’s spleen, GST activity was increased compared to control but γ-GCS expression was not affected. In OMW group’s liver, GST activity was increased and γ-GCS expression was reduced compared to control. In OMW group’s spleen, GST activity was increased but GCS expression was not affected. SOD activity was not affected in both tissues either in GP or OMW group.
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spelling pubmed-56151542017-09-28 Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep Kerasioti, Efthalia Terzopoulou, Zoi Komini, Ourania Kafantaris, Ioannis Makri, Sotiria Stagos, Dimitrios Gerasopoulos, Konstantinos Anisimov, Nikita Y. Tsatsakis, Aristides M. Kouretas, Demetrios Toxicol Rep Full Length Article The aim of the present study was to investigate the effects of livestock feed supplemented with grape pomace (GP) or olive oil mill wastewater (OMW) byproducts on the enzymatic activity and protein expression of antioxidants enzymes, in liver and spleen tissue of sheep. Thus, 36 male sheep of Chios breed were divided into 3 homogeneous groups, control group (n = 12), GP group (n = 12) and OMW group (n = 12), receiving standard or experimental feed. Liver and spleen tissues were collected at 42 and 70 days post-birth. The enzymatic activity of superoxide dismutase (SOD) and glutathione-s-transferase (GST) and also the protein expression of γ-synthase glutamyl custeine (γ-GCS) were determined in these tissues. The results showed GP group exhibited increased enzymatic activity of GST and protein expression of γ-GCS in liver compared to control group. In GP group’s spleen, GST activity was increased compared to control but γ-GCS expression was not affected. In OMW group’s liver, GST activity was increased and γ-GCS expression was reduced compared to control. In OMW group’s spleen, GST activity was increased but GCS expression was not affected. SOD activity was not affected in both tissues either in GP or OMW group. Elsevier 2017-06-23 /pmc/articles/PMC5615154/ /pubmed/28959661 http://dx.doi.org/10.1016/j.toxrep.2017.06.007 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Kerasioti, Efthalia
Terzopoulou, Zoi
Komini, Ourania
Kafantaris, Ioannis
Makri, Sotiria
Stagos, Dimitrios
Gerasopoulos, Konstantinos
Anisimov, Nikita Y.
Tsatsakis, Aristides M.
Kouretas, Demetrios
Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep
title Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep
title_full Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep
title_fullStr Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep
title_full_unstemmed Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep
title_short Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep
title_sort tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615154/
https://www.ncbi.nlm.nih.gov/pubmed/28959661
http://dx.doi.org/10.1016/j.toxrep.2017.06.007
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