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Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line
Farnesiferol C is one of the major compounds, isolated from Ferula asafoetida (a type of coumarins) and used for cancer treatment as a folk remedy. Treatment of many cancers depends on oxidative stress situation. In this study, we sought the hypothesis that oxidative stress induced by Farnesiferol C...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615162/ https://www.ncbi.nlm.nih.gov/pubmed/28959668 http://dx.doi.org/10.1016/j.toxrep.2017.07.010 |
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author | Hasanzadeh, Davoud Mahdavi, Majid Dehghan, Gholamreza Charoudeh, Hojjatollah Nozad |
author_facet | Hasanzadeh, Davoud Mahdavi, Majid Dehghan, Gholamreza Charoudeh, Hojjatollah Nozad |
author_sort | Hasanzadeh, Davoud |
collection | PubMed |
description | Farnesiferol C is one of the major compounds, isolated from Ferula asafoetida (a type of coumarins) and used for cancer treatment as a folk remedy. Treatment of many cancers depends on oxidative stress situation. In this study, we sought the hypothesis that oxidative stress induced by Farnesiferol C contribute to anticancer property and induce apoptosis in MCF-7, human breast cancer cell line. We investigated the effect of Farnesiferol C on oxidative stress by measurement of some enzymes activity including catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), as well as some parameters such as total thiol and ROS levels. Also we evaluated Farnesiferol C effects on the cell cycle and apoptosis induction by using flow cytometry analysis. Our findings demonstrated that Farnesiferol C significantly induced apoptosis mediated by increasing in the cellular ROS levels. This compound increased cellular SOD and CAT activities in 24 and 48 h and reduced activity of these enzymes after 72 h exposure. Furthermore, MDA and total thiol levels were increased and decreased, respectively in the cells treated with Farnesiferol C after 24–72 h. G0/G1 phase cell cycle arrest followed by induction of apoptosis was also observed in MCF-7 cells after treatment with Farnesiferol C. According to these data, Farnesiferol C has a therapeutic effect on MCF-7 cells and can be suitable candidate for breast cancer treatment; however it is necessary for further experiments. |
format | Online Article Text |
id | pubmed-5615162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-56151622017-09-28 Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line Hasanzadeh, Davoud Mahdavi, Majid Dehghan, Gholamreza Charoudeh, Hojjatollah Nozad Toxicol Rep Full Length Article Farnesiferol C is one of the major compounds, isolated from Ferula asafoetida (a type of coumarins) and used for cancer treatment as a folk remedy. Treatment of many cancers depends on oxidative stress situation. In this study, we sought the hypothesis that oxidative stress induced by Farnesiferol C contribute to anticancer property and induce apoptosis in MCF-7, human breast cancer cell line. We investigated the effect of Farnesiferol C on oxidative stress by measurement of some enzymes activity including catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), as well as some parameters such as total thiol and ROS levels. Also we evaluated Farnesiferol C effects on the cell cycle and apoptosis induction by using flow cytometry analysis. Our findings demonstrated that Farnesiferol C significantly induced apoptosis mediated by increasing in the cellular ROS levels. This compound increased cellular SOD and CAT activities in 24 and 48 h and reduced activity of these enzymes after 72 h exposure. Furthermore, MDA and total thiol levels were increased and decreased, respectively in the cells treated with Farnesiferol C after 24–72 h. G0/G1 phase cell cycle arrest followed by induction of apoptosis was also observed in MCF-7 cells after treatment with Farnesiferol C. According to these data, Farnesiferol C has a therapeutic effect on MCF-7 cells and can be suitable candidate for breast cancer treatment; however it is necessary for further experiments. Elsevier 2017-08-01 /pmc/articles/PMC5615162/ /pubmed/28959668 http://dx.doi.org/10.1016/j.toxrep.2017.07.010 Text en © 2017 Published by Elsevier Ireland Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Full Length Article Hasanzadeh, Davoud Mahdavi, Majid Dehghan, Gholamreza Charoudeh, Hojjatollah Nozad Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line |
title | Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line |
title_full | Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line |
title_fullStr | Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line |
title_full_unstemmed | Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line |
title_short | Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line |
title_sort | farnesiferol c induces cell cycle arrest and apoptosis mediated by oxidative stress in mcf-7 cell line |
topic | Full Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615162/ https://www.ncbi.nlm.nih.gov/pubmed/28959668 http://dx.doi.org/10.1016/j.toxrep.2017.07.010 |
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