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Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line

Farnesiferol C is one of the major compounds, isolated from Ferula asafoetida (a type of coumarins) and used for cancer treatment as a folk remedy. Treatment of many cancers depends on oxidative stress situation. In this study, we sought the hypothesis that oxidative stress induced by Farnesiferol C...

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Autores principales: Hasanzadeh, Davoud, Mahdavi, Majid, Dehghan, Gholamreza, Charoudeh, Hojjatollah Nozad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615162/
https://www.ncbi.nlm.nih.gov/pubmed/28959668
http://dx.doi.org/10.1016/j.toxrep.2017.07.010
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author Hasanzadeh, Davoud
Mahdavi, Majid
Dehghan, Gholamreza
Charoudeh, Hojjatollah Nozad
author_facet Hasanzadeh, Davoud
Mahdavi, Majid
Dehghan, Gholamreza
Charoudeh, Hojjatollah Nozad
author_sort Hasanzadeh, Davoud
collection PubMed
description Farnesiferol C is one of the major compounds, isolated from Ferula asafoetida (a type of coumarins) and used for cancer treatment as a folk remedy. Treatment of many cancers depends on oxidative stress situation. In this study, we sought the hypothesis that oxidative stress induced by Farnesiferol C contribute to anticancer property and induce apoptosis in MCF-7, human breast cancer cell line. We investigated the effect of Farnesiferol C on oxidative stress by measurement of some enzymes activity including catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), as well as some parameters such as total thiol and ROS levels. Also we evaluated Farnesiferol C effects on the cell cycle and apoptosis induction by using flow cytometry analysis. Our findings demonstrated that Farnesiferol C significantly induced apoptosis mediated by increasing in the cellular ROS levels. This compound increased cellular SOD and CAT activities in 24 and 48 h and reduced activity of these enzymes after 72 h exposure. Furthermore, MDA and total thiol levels were increased and decreased, respectively in the cells treated with Farnesiferol C after 24–72 h. G0/G1 phase cell cycle arrest followed by induction of apoptosis was also observed in MCF-7 cells after treatment with Farnesiferol C. According to these data, Farnesiferol C has a therapeutic effect on MCF-7 cells and can be suitable candidate for breast cancer treatment; however it is necessary for further experiments.
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spelling pubmed-56151622017-09-28 Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line Hasanzadeh, Davoud Mahdavi, Majid Dehghan, Gholamreza Charoudeh, Hojjatollah Nozad Toxicol Rep Full Length Article Farnesiferol C is one of the major compounds, isolated from Ferula asafoetida (a type of coumarins) and used for cancer treatment as a folk remedy. Treatment of many cancers depends on oxidative stress situation. In this study, we sought the hypothesis that oxidative stress induced by Farnesiferol C contribute to anticancer property and induce apoptosis in MCF-7, human breast cancer cell line. We investigated the effect of Farnesiferol C on oxidative stress by measurement of some enzymes activity including catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), as well as some parameters such as total thiol and ROS levels. Also we evaluated Farnesiferol C effects on the cell cycle and apoptosis induction by using flow cytometry analysis. Our findings demonstrated that Farnesiferol C significantly induced apoptosis mediated by increasing in the cellular ROS levels. This compound increased cellular SOD and CAT activities in 24 and 48 h and reduced activity of these enzymes after 72 h exposure. Furthermore, MDA and total thiol levels were increased and decreased, respectively in the cells treated with Farnesiferol C after 24–72 h. G0/G1 phase cell cycle arrest followed by induction of apoptosis was also observed in MCF-7 cells after treatment with Farnesiferol C. According to these data, Farnesiferol C has a therapeutic effect on MCF-7 cells and can be suitable candidate for breast cancer treatment; however it is necessary for further experiments. Elsevier 2017-08-01 /pmc/articles/PMC5615162/ /pubmed/28959668 http://dx.doi.org/10.1016/j.toxrep.2017.07.010 Text en © 2017 Published by Elsevier Ireland Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Hasanzadeh, Davoud
Mahdavi, Majid
Dehghan, Gholamreza
Charoudeh, Hojjatollah Nozad
Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line
title Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line
title_full Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line
title_fullStr Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line
title_full_unstemmed Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line
title_short Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line
title_sort farnesiferol c induces cell cycle arrest and apoptosis mediated by oxidative stress in mcf-7 cell line
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615162/
https://www.ncbi.nlm.nih.gov/pubmed/28959668
http://dx.doi.org/10.1016/j.toxrep.2017.07.010
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