Cargando…

Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients

Introduction: Pseudomonas aeruginosa infections are prevalent amongst chronic rhinosinusitis (CRS) sufferers. Many P. aeruginosa strains form biofilms, leading to treatment failure. Lytic bacteriophages (phages) are viruses that infect, replicate within, and lyse bacteria, causing bacterial death. A...

Descripción completa

Detalles Bibliográficos
Autores principales: Fong, Stephanie A., Drilling, Amanda, Morales, Sandra, Cornet, Marjolein E., Woodworth, Bradford A., Fokkens, Wytske J., Psaltis, Alkis J., Vreugde, Sarah, Wormald, Peter-John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615211/
https://www.ncbi.nlm.nih.gov/pubmed/29018773
http://dx.doi.org/10.3389/fcimb.2017.00418
_version_ 1783266540430295040
author Fong, Stephanie A.
Drilling, Amanda
Morales, Sandra
Cornet, Marjolein E.
Woodworth, Bradford A.
Fokkens, Wytske J.
Psaltis, Alkis J.
Vreugde, Sarah
Wormald, Peter-John
author_facet Fong, Stephanie A.
Drilling, Amanda
Morales, Sandra
Cornet, Marjolein E.
Woodworth, Bradford A.
Fokkens, Wytske J.
Psaltis, Alkis J.
Vreugde, Sarah
Wormald, Peter-John
author_sort Fong, Stephanie A.
collection PubMed
description Introduction: Pseudomonas aeruginosa infections are prevalent amongst chronic rhinosinusitis (CRS) sufferers. Many P. aeruginosa strains form biofilms, leading to treatment failure. Lytic bacteriophages (phages) are viruses that infect, replicate within, and lyse bacteria, causing bacterial death. Aim: To assess the activity of a phage cocktail in eradicating biofilms of ex vivo P.aeruginosa isolates from CRS patients. Methods: P. aeruginosa isolates from CRS patients with and without cystic fibrosis (CF) across three continents were multi-locus sequence typed and tested for antibiotic resistance. Biofilms grown in vitro were treated with a cocktail of four phages (CT-PA). Biofilm biomass was measured after 24 and 48 h, using a crystal violet assay. Phage titrations were performed to confirm replication of the phages. A linear mixed effects model was applied to assess the effects of treatment, time, CF status, and multidrug resistance on the biomass of the biofilm. Results: The isolates included 44 strain types. CT-PA treatment significantly reduced biofilm biomass at both 24 and 48 h post-treatment (p < 0.0001), regardless of CF status or antibiotic resistance. Biomass was decreased by a median of 76% at 48 h. Decrease in biofilm was accompanied by a rise in phage titres for all except one strain. Conclusion: A single dose of phages is able to significantly reduce biofilms formed in vitro by a range of P.aeruginosa isolates from CRS patients. This represents an exciting potential and novel targeted treatment for P. aeruginosa biofilm infections and multidrug resistant bacteria.
format Online
Article
Text
id pubmed-5615211
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-56152112017-10-10 Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients Fong, Stephanie A. Drilling, Amanda Morales, Sandra Cornet, Marjolein E. Woodworth, Bradford A. Fokkens, Wytske J. Psaltis, Alkis J. Vreugde, Sarah Wormald, Peter-John Front Cell Infect Microbiol Microbiology Introduction: Pseudomonas aeruginosa infections are prevalent amongst chronic rhinosinusitis (CRS) sufferers. Many P. aeruginosa strains form biofilms, leading to treatment failure. Lytic bacteriophages (phages) are viruses that infect, replicate within, and lyse bacteria, causing bacterial death. Aim: To assess the activity of a phage cocktail in eradicating biofilms of ex vivo P.aeruginosa isolates from CRS patients. Methods: P. aeruginosa isolates from CRS patients with and without cystic fibrosis (CF) across three continents were multi-locus sequence typed and tested for antibiotic resistance. Biofilms grown in vitro were treated with a cocktail of four phages (CT-PA). Biofilm biomass was measured after 24 and 48 h, using a crystal violet assay. Phage titrations were performed to confirm replication of the phages. A linear mixed effects model was applied to assess the effects of treatment, time, CF status, and multidrug resistance on the biomass of the biofilm. Results: The isolates included 44 strain types. CT-PA treatment significantly reduced biofilm biomass at both 24 and 48 h post-treatment (p < 0.0001), regardless of CF status or antibiotic resistance. Biomass was decreased by a median of 76% at 48 h. Decrease in biofilm was accompanied by a rise in phage titres for all except one strain. Conclusion: A single dose of phages is able to significantly reduce biofilms formed in vitro by a range of P.aeruginosa isolates from CRS patients. This represents an exciting potential and novel targeted treatment for P. aeruginosa biofilm infections and multidrug resistant bacteria. Frontiers Media S.A. 2017-09-22 /pmc/articles/PMC5615211/ /pubmed/29018773 http://dx.doi.org/10.3389/fcimb.2017.00418 Text en Copyright © 2017 Fong, Drilling, Morales, Cornet, Woodworth, Fokkens, Psaltis, Vreugde and Wormald. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Fong, Stephanie A.
Drilling, Amanda
Morales, Sandra
Cornet, Marjolein E.
Woodworth, Bradford A.
Fokkens, Wytske J.
Psaltis, Alkis J.
Vreugde, Sarah
Wormald, Peter-John
Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
title Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
title_full Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
title_fullStr Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
title_full_unstemmed Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
title_short Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
title_sort activity of bacteriophages in removing biofilms of pseudomonas aeruginosa isolates from chronic rhinosinusitis patients
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615211/
https://www.ncbi.nlm.nih.gov/pubmed/29018773
http://dx.doi.org/10.3389/fcimb.2017.00418
work_keys_str_mv AT fongstephaniea activityofbacteriophagesinremovingbiofilmsofpseudomonasaeruginosaisolatesfromchronicrhinosinusitispatients
AT drillingamanda activityofbacteriophagesinremovingbiofilmsofpseudomonasaeruginosaisolatesfromchronicrhinosinusitispatients
AT moralessandra activityofbacteriophagesinremovingbiofilmsofpseudomonasaeruginosaisolatesfromchronicrhinosinusitispatients
AT cornetmarjoleine activityofbacteriophagesinremovingbiofilmsofpseudomonasaeruginosaisolatesfromchronicrhinosinusitispatients
AT woodworthbradforda activityofbacteriophagesinremovingbiofilmsofpseudomonasaeruginosaisolatesfromchronicrhinosinusitispatients
AT fokkenswytskej activityofbacteriophagesinremovingbiofilmsofpseudomonasaeruginosaisolatesfromchronicrhinosinusitispatients
AT psaltisalkisj activityofbacteriophagesinremovingbiofilmsofpseudomonasaeruginosaisolatesfromchronicrhinosinusitispatients
AT vreugdesarah activityofbacteriophagesinremovingbiofilmsofpseudomonasaeruginosaisolatesfromchronicrhinosinusitispatients
AT wormaldpeterjohn activityofbacteriophagesinremovingbiofilmsofpseudomonasaeruginosaisolatesfromchronicrhinosinusitispatients