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Gut Microbiota-Dependent Trimethylamine-N-oxide and Serum Biomarkers in Patients with T2DM and Advanced CKD

Trimethylamine-N-oxide (TMAO) is a product of dietary, gut microbiome, and tissues metabolism. Elevated blood TMAO levels are associated with heart attack, stroke and chronic kidney disease (CKD). The purpose of our study was to investigate the gut microbiota associated with trimethylamine (TMA) pro...

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Autores principales: Al-Obaide, Mohammed A. I., Singh, Ruchi, Datta, Palika, Rewers-Felkins, Kathy A., Salguero, Maria V., Al-Obaidi, Ibtisam, Kottapalli, Kameswara Rao, Vasylyeva, Tetyana L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615279/
https://www.ncbi.nlm.nih.gov/pubmed/28925931
http://dx.doi.org/10.3390/jcm6090086
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author Al-Obaide, Mohammed A. I.
Singh, Ruchi
Datta, Palika
Rewers-Felkins, Kathy A.
Salguero, Maria V.
Al-Obaidi, Ibtisam
Kottapalli, Kameswara Rao
Vasylyeva, Tetyana L.
author_facet Al-Obaide, Mohammed A. I.
Singh, Ruchi
Datta, Palika
Rewers-Felkins, Kathy A.
Salguero, Maria V.
Al-Obaidi, Ibtisam
Kottapalli, Kameswara Rao
Vasylyeva, Tetyana L.
author_sort Al-Obaide, Mohammed A. I.
collection PubMed
description Trimethylamine-N-oxide (TMAO) is a product of dietary, gut microbiome, and tissues metabolism. Elevated blood TMAO levels are associated with heart attack, stroke and chronic kidney disease (CKD). The purpose of our study was to investigate the gut microbiota associated with trimethylamine (TMA) production, the precursor of TMAO, and the serum levels of TMAO and inflammatory biomarkers associated with type 2 diabetes mellitus (T2DM) and CKD. Twenty adults with T2DM and advanced CKD and 20 healthy adults participated in the study. Analyses included anthropometric and metabolic parameters, characterization of TMA producing gut microbiota, and concentrations of TMAO, lipopolysaccharides (LPS) endotoxin, zonulin (Zo) gut permeability marker, and serum inflammatory and endothelial dysfunction biomarkers. Diversity of the gut microbiota was identified by amplification of V3–V4 regions of the 16S ribosomal RNA genes and DNA sequencing. TMAO was quantified by Mass Spectrometry and serum biomarkers by ELISA. The significance of measurements justified by statistical analysis. The gut microbiome in T2DM-CKD patients exhibited a higher incidence of TMA-producing bacteria than control, p < 0.05. The serum levels of TMAO in T2DM-CKD patients were significantly higher than controls, p < 0.05. TMAO showed a positive correlation with Zo and LPS, inflammatory and endothelial dysfunction biomarkers. A positive correlation was observed between Zo and LPS in T2DM-CKD subjects. An increased abundance of TMA-producing bacteria in the gut microbiota of T2DM-CKD patients together with excessive TMAO and increased gut permeability might impact their risk for cardiovascular disease through elevation of chronic inflammation and endothelial dysfunction.
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spelling pubmed-56152792017-09-28 Gut Microbiota-Dependent Trimethylamine-N-oxide and Serum Biomarkers in Patients with T2DM and Advanced CKD Al-Obaide, Mohammed A. I. Singh, Ruchi Datta, Palika Rewers-Felkins, Kathy A. Salguero, Maria V. Al-Obaidi, Ibtisam Kottapalli, Kameswara Rao Vasylyeva, Tetyana L. J Clin Med Article Trimethylamine-N-oxide (TMAO) is a product of dietary, gut microbiome, and tissues metabolism. Elevated blood TMAO levels are associated with heart attack, stroke and chronic kidney disease (CKD). The purpose of our study was to investigate the gut microbiota associated with trimethylamine (TMA) production, the precursor of TMAO, and the serum levels of TMAO and inflammatory biomarkers associated with type 2 diabetes mellitus (T2DM) and CKD. Twenty adults with T2DM and advanced CKD and 20 healthy adults participated in the study. Analyses included anthropometric and metabolic parameters, characterization of TMA producing gut microbiota, and concentrations of TMAO, lipopolysaccharides (LPS) endotoxin, zonulin (Zo) gut permeability marker, and serum inflammatory and endothelial dysfunction biomarkers. Diversity of the gut microbiota was identified by amplification of V3–V4 regions of the 16S ribosomal RNA genes and DNA sequencing. TMAO was quantified by Mass Spectrometry and serum biomarkers by ELISA. The significance of measurements justified by statistical analysis. The gut microbiome in T2DM-CKD patients exhibited a higher incidence of TMA-producing bacteria than control, p < 0.05. The serum levels of TMAO in T2DM-CKD patients were significantly higher than controls, p < 0.05. TMAO showed a positive correlation with Zo and LPS, inflammatory and endothelial dysfunction biomarkers. A positive correlation was observed between Zo and LPS in T2DM-CKD subjects. An increased abundance of TMA-producing bacteria in the gut microbiota of T2DM-CKD patients together with excessive TMAO and increased gut permeability might impact their risk for cardiovascular disease through elevation of chronic inflammation and endothelial dysfunction. MDPI 2017-09-19 /pmc/articles/PMC5615279/ /pubmed/28925931 http://dx.doi.org/10.3390/jcm6090086 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Obaide, Mohammed A. I.
Singh, Ruchi
Datta, Palika
Rewers-Felkins, Kathy A.
Salguero, Maria V.
Al-Obaidi, Ibtisam
Kottapalli, Kameswara Rao
Vasylyeva, Tetyana L.
Gut Microbiota-Dependent Trimethylamine-N-oxide and Serum Biomarkers in Patients with T2DM and Advanced CKD
title Gut Microbiota-Dependent Trimethylamine-N-oxide and Serum Biomarkers in Patients with T2DM and Advanced CKD
title_full Gut Microbiota-Dependent Trimethylamine-N-oxide and Serum Biomarkers in Patients with T2DM and Advanced CKD
title_fullStr Gut Microbiota-Dependent Trimethylamine-N-oxide and Serum Biomarkers in Patients with T2DM and Advanced CKD
title_full_unstemmed Gut Microbiota-Dependent Trimethylamine-N-oxide and Serum Biomarkers in Patients with T2DM and Advanced CKD
title_short Gut Microbiota-Dependent Trimethylamine-N-oxide and Serum Biomarkers in Patients with T2DM and Advanced CKD
title_sort gut microbiota-dependent trimethylamine-n-oxide and serum biomarkers in patients with t2dm and advanced ckd
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615279/
https://www.ncbi.nlm.nih.gov/pubmed/28925931
http://dx.doi.org/10.3390/jcm6090086
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