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Measurement of Respiratory Chain Enzyme Activity in Human Renal Biopsy Specimens

Background: Mitochondrial disorders can present as kidney disease in children and be difficult to diagnose. Measurement of mitochondrial function in kidney tissue may help diagnosis. This study was to assess the feasibility of obtaining renal samples and analysing them for respiratory chain enzyme a...

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Autores principales: Ghose, Arun, Taylor, Christopher M., Howie, Alexander J., Chalasani, Anapurna, Hargreaves, Iain, Milford, David V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615283/
https://www.ncbi.nlm.nih.gov/pubmed/28925945
http://dx.doi.org/10.3390/jcm6090090
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author Ghose, Arun
Taylor, Christopher M.
Howie, Alexander J.
Chalasani, Anapurna
Hargreaves, Iain
Milford, David V.
author_facet Ghose, Arun
Taylor, Christopher M.
Howie, Alexander J.
Chalasani, Anapurna
Hargreaves, Iain
Milford, David V.
author_sort Ghose, Arun
collection PubMed
description Background: Mitochondrial disorders can present as kidney disease in children and be difficult to diagnose. Measurement of mitochondrial function in kidney tissue may help diagnosis. This study was to assess the feasibility of obtaining renal samples and analysing them for respiratory chain enzyme activity. Methods: The subjects were children undergoing a routine diagnostic renal biopsy, in whom a clinical condition of renal inflammation, scarring and primary metabolic disorder was unlikely. A fresh sample of kidney was snap frozen and later assayed for the activities of respiratory chain enzyme complexes I, II/III, and IV using spectrophotometric enzyme assay, and expressed as a ratio of citrate synthase activity. Results: The range of respiratory chain enzyme activity for complex I was 0.161 to 0.866 (mean 0.404, SD 0.2), for complex II/III was 0.021 to 0.318 (mean 0.177, SD 0.095) and for complex IV was 0.001 to 0.025 (mean 0.015, SD 0.006). There were correlations between the different activities but not between them and the age of the children or a measure of the amount of chronic damage in the kidneys. Conclusion: It is feasible to measure respiratory chain enzyme activity in routine renal biopsy specimens.
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spelling pubmed-56152832017-09-28 Measurement of Respiratory Chain Enzyme Activity in Human Renal Biopsy Specimens Ghose, Arun Taylor, Christopher M. Howie, Alexander J. Chalasani, Anapurna Hargreaves, Iain Milford, David V. J Clin Med Article Background: Mitochondrial disorders can present as kidney disease in children and be difficult to diagnose. Measurement of mitochondrial function in kidney tissue may help diagnosis. This study was to assess the feasibility of obtaining renal samples and analysing them for respiratory chain enzyme activity. Methods: The subjects were children undergoing a routine diagnostic renal biopsy, in whom a clinical condition of renal inflammation, scarring and primary metabolic disorder was unlikely. A fresh sample of kidney was snap frozen and later assayed for the activities of respiratory chain enzyme complexes I, II/III, and IV using spectrophotometric enzyme assay, and expressed as a ratio of citrate synthase activity. Results: The range of respiratory chain enzyme activity for complex I was 0.161 to 0.866 (mean 0.404, SD 0.2), for complex II/III was 0.021 to 0.318 (mean 0.177, SD 0.095) and for complex IV was 0.001 to 0.025 (mean 0.015, SD 0.006). There were correlations between the different activities but not between them and the age of the children or a measure of the amount of chronic damage in the kidneys. Conclusion: It is feasible to measure respiratory chain enzyme activity in routine renal biopsy specimens. MDPI 2017-09-19 /pmc/articles/PMC5615283/ /pubmed/28925945 http://dx.doi.org/10.3390/jcm6090090 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ghose, Arun
Taylor, Christopher M.
Howie, Alexander J.
Chalasani, Anapurna
Hargreaves, Iain
Milford, David V.
Measurement of Respiratory Chain Enzyme Activity in Human Renal Biopsy Specimens
title Measurement of Respiratory Chain Enzyme Activity in Human Renal Biopsy Specimens
title_full Measurement of Respiratory Chain Enzyme Activity in Human Renal Biopsy Specimens
title_fullStr Measurement of Respiratory Chain Enzyme Activity in Human Renal Biopsy Specimens
title_full_unstemmed Measurement of Respiratory Chain Enzyme Activity in Human Renal Biopsy Specimens
title_short Measurement of Respiratory Chain Enzyme Activity in Human Renal Biopsy Specimens
title_sort measurement of respiratory chain enzyme activity in human renal biopsy specimens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615283/
https://www.ncbi.nlm.nih.gov/pubmed/28925945
http://dx.doi.org/10.3390/jcm6090090
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