Cargando…

Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction

Mechanotransduction between cells and the extracellular matrix regulates major cellular functions in physiological and pathological situations. The effect of mechanical cues on biochemical signaling triggered by cell–matrix and cell–cell interactions on model biomimetic surfaces has been extensively...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Ying, Liao, Kin, Li, Chuan, Lai, Alvin C.K., Foo, Ji-Jinn, Chan, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615318/
https://www.ncbi.nlm.nih.gov/pubmed/28952551
http://dx.doi.org/10.3390/bioengineering4030072
_version_ 1783266564445831168
author Zhang, Ying
Liao, Kin
Li, Chuan
Lai, Alvin C.K.
Foo, Ji-Jinn
Chan, Vincent
author_facet Zhang, Ying
Liao, Kin
Li, Chuan
Lai, Alvin C.K.
Foo, Ji-Jinn
Chan, Vincent
author_sort Zhang, Ying
collection PubMed
description Mechanotransduction between cells and the extracellular matrix regulates major cellular functions in physiological and pathological situations. The effect of mechanical cues on biochemical signaling triggered by cell–matrix and cell–cell interactions on model biomimetic surfaces has been extensively investigated by a combination of fabrication, biophysical, and biological methods. To simulate the in vivo physiological microenvironment in vitro, three dimensional (3D) microstructures with tailored bio-functionality have been fabricated on substrates of various materials. However, less attention has been paid to the design of 3D biomaterial systems with geometric variances, such as the possession of precise micro-features and/or bio-sensing elements for probing the mechanical responses of cells to the external microenvironment. Such precisely engineered 3D model experimental platforms pave the way for studying the mechanotransduction of multicellular aggregates under controlled geometric and mechanical parameters. Concurrently with the progress in 3D biomaterial fabrication, cell traction force microscopy (CTFM) developed in the field of cell biophysics has emerged as a highly sensitive technique for probing the mechanical stresses exerted by cells onto the opposing deformable surface. In the current work, we first review the recent advances in the fabrication of 3D micropatterned biomaterials which enable the seamless integration with experimental cell mechanics in a controlled 3D microenvironment. Then, we discuss the role of collective cell–cell interactions in the mechanotransduction of engineered tissue equivalents determined by such integrative biomaterial systems under simulated physiological conditions.
format Online
Article
Text
id pubmed-5615318
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-56153182017-09-28 Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction Zhang, Ying Liao, Kin Li, Chuan Lai, Alvin C.K. Foo, Ji-Jinn Chan, Vincent Bioengineering (Basel) Review Mechanotransduction between cells and the extracellular matrix regulates major cellular functions in physiological and pathological situations. The effect of mechanical cues on biochemical signaling triggered by cell–matrix and cell–cell interactions on model biomimetic surfaces has been extensively investigated by a combination of fabrication, biophysical, and biological methods. To simulate the in vivo physiological microenvironment in vitro, three dimensional (3D) microstructures with tailored bio-functionality have been fabricated on substrates of various materials. However, less attention has been paid to the design of 3D biomaterial systems with geometric variances, such as the possession of precise micro-features and/or bio-sensing elements for probing the mechanical responses of cells to the external microenvironment. Such precisely engineered 3D model experimental platforms pave the way for studying the mechanotransduction of multicellular aggregates under controlled geometric and mechanical parameters. Concurrently with the progress in 3D biomaterial fabrication, cell traction force microscopy (CTFM) developed in the field of cell biophysics has emerged as a highly sensitive technique for probing the mechanical stresses exerted by cells onto the opposing deformable surface. In the current work, we first review the recent advances in the fabrication of 3D micropatterned biomaterials which enable the seamless integration with experimental cell mechanics in a controlled 3D microenvironment. Then, we discuss the role of collective cell–cell interactions in the mechanotransduction of engineered tissue equivalents determined by such integrative biomaterial systems under simulated physiological conditions. MDPI 2017-08-24 /pmc/articles/PMC5615318/ /pubmed/28952551 http://dx.doi.org/10.3390/bioengineering4030072 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Zhang, Ying
Liao, Kin
Li, Chuan
Lai, Alvin C.K.
Foo, Ji-Jinn
Chan, Vincent
Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction
title Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction
title_full Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction
title_fullStr Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction
title_full_unstemmed Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction
title_short Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction
title_sort progress in integrative biomaterial systems to approach three-dimensional cell mechanotransduction
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615318/
https://www.ncbi.nlm.nih.gov/pubmed/28952551
http://dx.doi.org/10.3390/bioengineering4030072
work_keys_str_mv AT zhangying progressinintegrativebiomaterialsystemstoapproachthreedimensionalcellmechanotransduction
AT liaokin progressinintegrativebiomaterialsystemstoapproachthreedimensionalcellmechanotransduction
AT lichuan progressinintegrativebiomaterialsystemstoapproachthreedimensionalcellmechanotransduction
AT laialvinck progressinintegrativebiomaterialsystemstoapproachthreedimensionalcellmechanotransduction
AT foojijinn progressinintegrativebiomaterialsystemstoapproachthreedimensionalcellmechanotransduction
AT chanvincent progressinintegrativebiomaterialsystemstoapproachthreedimensionalcellmechanotransduction