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Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction
Mechanotransduction between cells and the extracellular matrix regulates major cellular functions in physiological and pathological situations. The effect of mechanical cues on biochemical signaling triggered by cell–matrix and cell–cell interactions on model biomimetic surfaces has been extensively...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615318/ https://www.ncbi.nlm.nih.gov/pubmed/28952551 http://dx.doi.org/10.3390/bioengineering4030072 |
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author | Zhang, Ying Liao, Kin Li, Chuan Lai, Alvin C.K. Foo, Ji-Jinn Chan, Vincent |
author_facet | Zhang, Ying Liao, Kin Li, Chuan Lai, Alvin C.K. Foo, Ji-Jinn Chan, Vincent |
author_sort | Zhang, Ying |
collection | PubMed |
description | Mechanotransduction between cells and the extracellular matrix regulates major cellular functions in physiological and pathological situations. The effect of mechanical cues on biochemical signaling triggered by cell–matrix and cell–cell interactions on model biomimetic surfaces has been extensively investigated by a combination of fabrication, biophysical, and biological methods. To simulate the in vivo physiological microenvironment in vitro, three dimensional (3D) microstructures with tailored bio-functionality have been fabricated on substrates of various materials. However, less attention has been paid to the design of 3D biomaterial systems with geometric variances, such as the possession of precise micro-features and/or bio-sensing elements for probing the mechanical responses of cells to the external microenvironment. Such precisely engineered 3D model experimental platforms pave the way for studying the mechanotransduction of multicellular aggregates under controlled geometric and mechanical parameters. Concurrently with the progress in 3D biomaterial fabrication, cell traction force microscopy (CTFM) developed in the field of cell biophysics has emerged as a highly sensitive technique for probing the mechanical stresses exerted by cells onto the opposing deformable surface. In the current work, we first review the recent advances in the fabrication of 3D micropatterned biomaterials which enable the seamless integration with experimental cell mechanics in a controlled 3D microenvironment. Then, we discuss the role of collective cell–cell interactions in the mechanotransduction of engineered tissue equivalents determined by such integrative biomaterial systems under simulated physiological conditions. |
format | Online Article Text |
id | pubmed-5615318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56153182017-09-28 Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction Zhang, Ying Liao, Kin Li, Chuan Lai, Alvin C.K. Foo, Ji-Jinn Chan, Vincent Bioengineering (Basel) Review Mechanotransduction between cells and the extracellular matrix regulates major cellular functions in physiological and pathological situations. The effect of mechanical cues on biochemical signaling triggered by cell–matrix and cell–cell interactions on model biomimetic surfaces has been extensively investigated by a combination of fabrication, biophysical, and biological methods. To simulate the in vivo physiological microenvironment in vitro, three dimensional (3D) microstructures with tailored bio-functionality have been fabricated on substrates of various materials. However, less attention has been paid to the design of 3D biomaterial systems with geometric variances, such as the possession of precise micro-features and/or bio-sensing elements for probing the mechanical responses of cells to the external microenvironment. Such precisely engineered 3D model experimental platforms pave the way for studying the mechanotransduction of multicellular aggregates under controlled geometric and mechanical parameters. Concurrently with the progress in 3D biomaterial fabrication, cell traction force microscopy (CTFM) developed in the field of cell biophysics has emerged as a highly sensitive technique for probing the mechanical stresses exerted by cells onto the opposing deformable surface. In the current work, we first review the recent advances in the fabrication of 3D micropatterned biomaterials which enable the seamless integration with experimental cell mechanics in a controlled 3D microenvironment. Then, we discuss the role of collective cell–cell interactions in the mechanotransduction of engineered tissue equivalents determined by such integrative biomaterial systems under simulated physiological conditions. MDPI 2017-08-24 /pmc/articles/PMC5615318/ /pubmed/28952551 http://dx.doi.org/10.3390/bioengineering4030072 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Zhang, Ying Liao, Kin Li, Chuan Lai, Alvin C.K. Foo, Ji-Jinn Chan, Vincent Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction |
title | Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction |
title_full | Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction |
title_fullStr | Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction |
title_full_unstemmed | Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction |
title_short | Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction |
title_sort | progress in integrative biomaterial systems to approach three-dimensional cell mechanotransduction |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615318/ https://www.ncbi.nlm.nih.gov/pubmed/28952551 http://dx.doi.org/10.3390/bioengineering4030072 |
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