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Enhanced Mutant Screening in One-step PCR-based Multiple Site-directed Plasmid Mutagenesis by Introduction of Silent Restriction Sites for Structural and Functional Study of Proteins

Site-directed mutagenesis (SDM) has been widely used for studying the structure and function of proteins. A one-step polymerase chain reaction (PCR)-based multiple site-directed plasmid mutagenesis method with extended non-overlapping sequence at the 3′ end of the primer increases the PCR amplificat...

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Detalles Bibliográficos
Autores principales:	Kuo, Ting-Yu, Tsai, Chung-Che, Fu, Hua-Wen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Short Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615473/ https://www.ncbi.nlm.nih.gov/pubmed/28959142 http://dx.doi.org/10.1186/s12575-017-0062-5

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