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Protein unfolding mechanisms and their effects on folding experiments
In this review, I discuss the various methods researchers use to unfold proteins in the lab in order to understand protein folding both in vitro and in vivo. The four main techniques, chemical-, heat-, pressure- and force-denaturation, produce distinctly different unfolded conformational ensembles....
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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F1000Research
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615768/ https://www.ncbi.nlm.nih.gov/pubmed/29034084 http://dx.doi.org/10.12688/f1000research.12070.1 |