Isolation of bacteriophages against multidrug resistant Acinetobacter baumannii

Increasing multiple drug resistant (MDR) strains of Acinetobacter baumannii has aggravated curiosity in development of alternative therapy. Bacteriophages are often considered as alternative agents for controlling A. baumannii infections. In the present study two lytic phages for MDR A. baumannii we...

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Autores principales: Ghajavand, Hasan, Esfahani, Bahram Nasr, Havaei, Asghar, Fazeli, Hossein, Jafari, Reyhaneh, Moghim, Sharareh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615867/
https://www.ncbi.nlm.nih.gov/pubmed/28974975
http://dx.doi.org/10.4103/1735-5362.213982
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author Ghajavand, Hasan
Esfahani, Bahram Nasr
Havaei, Asghar
Fazeli, Hossein
Jafari, Reyhaneh
Moghim, Sharareh
author_facet Ghajavand, Hasan
Esfahani, Bahram Nasr
Havaei, Asghar
Fazeli, Hossein
Jafari, Reyhaneh
Moghim, Sharareh
author_sort Ghajavand, Hasan
collection PubMed
description Increasing multiple drug resistant (MDR) strains of Acinetobacter baumannii has aggravated curiosity in development of alternative therapy. Bacteriophages are often considered as alternative agents for controlling A. baumannii infections. In the present study two lytic phages for MDR A. baumannii were isolated and their efficacy and host ranges were evaluated. The phages were isolated from hospital wastewater. Electron microscopy revealed that IsfAB78 might belong to Myoviridae and IsfAB39 to Podoviridae. Initial characterization of phages showed that they have narrow host range and failed to infect relative and non-relative bacteria. Both phages decreased the A. baumannii turbidity significantly, indicating that these isolated phages may be considered as candidates for phage therapy.
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spelling pubmed-56158672017-10-04 Isolation of bacteriophages against multidrug resistant Acinetobacter baumannii Ghajavand, Hasan Esfahani, Bahram Nasr Havaei, Asghar Fazeli, Hossein Jafari, Reyhaneh Moghim, Sharareh Res Pharm Sci Original Article Increasing multiple drug resistant (MDR) strains of Acinetobacter baumannii has aggravated curiosity in development of alternative therapy. Bacteriophages are often considered as alternative agents for controlling A. baumannii infections. In the present study two lytic phages for MDR A. baumannii were isolated and their efficacy and host ranges were evaluated. The phages were isolated from hospital wastewater. Electron microscopy revealed that IsfAB78 might belong to Myoviridae and IsfAB39 to Podoviridae. Initial characterization of phages showed that they have narrow host range and failed to infect relative and non-relative bacteria. Both phages decreased the A. baumannii turbidity significantly, indicating that these isolated phages may be considered as candidates for phage therapy. Medknow Publications & Media Pvt Ltd 2017-10 /pmc/articles/PMC5615867/ /pubmed/28974975 http://dx.doi.org/10.4103/1735-5362.213982 Text en Copyright: © 2017 Research in Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Ghajavand, Hasan
Esfahani, Bahram Nasr
Havaei, Asghar
Fazeli, Hossein
Jafari, Reyhaneh
Moghim, Sharareh
Isolation of bacteriophages against multidrug resistant Acinetobacter baumannii
title Isolation of bacteriophages against multidrug resistant Acinetobacter baumannii
title_full Isolation of bacteriophages against multidrug resistant Acinetobacter baumannii
title_fullStr Isolation of bacteriophages against multidrug resistant Acinetobacter baumannii
title_full_unstemmed Isolation of bacteriophages against multidrug resistant Acinetobacter baumannii
title_short Isolation of bacteriophages against multidrug resistant Acinetobacter baumannii
title_sort isolation of bacteriophages against multidrug resistant acinetobacter baumannii
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615867/
https://www.ncbi.nlm.nih.gov/pubmed/28974975
http://dx.doi.org/10.4103/1735-5362.213982
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