WRKY45 phosphorylation at threonine 266 acts negatively on WRKY45-dependent blast resistance in rice

WRKY45 is a central regulator of disease resistance mediated by salicylic acid signaling in rice and its activation involves phosphorylation by OsMPK6. OsMPK6 phosphorylates WRKY45 at Thr266, Ser294, and Ser299 in vitro. Phosphorylation of Ser294 and/or Ser299 is required for full activation of WRKY...

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Autores principales: Ueno, Yoshihisa, Matsushita, Akane, Inoue, Haruhiko, Yoshida, Riichiro, Jiang, Chang-Jie, Takatsuji, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5616141/
https://www.ncbi.nlm.nih.gov/pubmed/28758876
http://dx.doi.org/10.1080/15592324.2017.1356968
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author Ueno, Yoshihisa
Matsushita, Akane
Inoue, Haruhiko
Yoshida, Riichiro
Jiang, Chang-Jie
Takatsuji, Hiroshi
author_facet Ueno, Yoshihisa
Matsushita, Akane
Inoue, Haruhiko
Yoshida, Riichiro
Jiang, Chang-Jie
Takatsuji, Hiroshi
author_sort Ueno, Yoshihisa
collection PubMed
description WRKY45 is a central regulator of disease resistance mediated by salicylic acid signaling in rice and its activation involves phosphorylation by OsMPK6. OsMPK6 phosphorylates WRKY45 at Thr266, Ser294, and Ser299 in vitro. Phosphorylation of Ser294 and/or Ser299 is required for full activation of WRKY45, but the importance of Thr266 phosphorylation has remained unknown. Here, we report on the characterization of Thr266 phosphorylation of WRKY45 in rice. Transient expression of mutant WRKY45 revealed that Thr266 is phosphorylated in vivo, together with Ser294/299. Replacement of Thr266 by Asn did not affect the enhanced Magnaporthe oryzae resistance afforded by WRKY45 overexpression. By contrast, replacement by Asp negated the enhancement of M. oryzae resistance. These results suggest that Thr266 phosphorylation acts negatively on WRKY45-dependent disease resistance.
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spelling pubmed-56161412017-10-02 WRKY45 phosphorylation at threonine 266 acts negatively on WRKY45-dependent blast resistance in rice Ueno, Yoshihisa Matsushita, Akane Inoue, Haruhiko Yoshida, Riichiro Jiang, Chang-Jie Takatsuji, Hiroshi Plant Signal Behav Short Communication WRKY45 is a central regulator of disease resistance mediated by salicylic acid signaling in rice and its activation involves phosphorylation by OsMPK6. OsMPK6 phosphorylates WRKY45 at Thr266, Ser294, and Ser299 in vitro. Phosphorylation of Ser294 and/or Ser299 is required for full activation of WRKY45, but the importance of Thr266 phosphorylation has remained unknown. Here, we report on the characterization of Thr266 phosphorylation of WRKY45 in rice. Transient expression of mutant WRKY45 revealed that Thr266 is phosphorylated in vivo, together with Ser294/299. Replacement of Thr266 by Asn did not affect the enhanced Magnaporthe oryzae resistance afforded by WRKY45 overexpression. By contrast, replacement by Asp negated the enhancement of M. oryzae resistance. These results suggest that Thr266 phosphorylation acts negatively on WRKY45-dependent disease resistance. Taylor & Francis 2017-07-31 /pmc/articles/PMC5616141/ /pubmed/28758876 http://dx.doi.org/10.1080/15592324.2017.1356968 Text en © 2017 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Short Communication
Ueno, Yoshihisa
Matsushita, Akane
Inoue, Haruhiko
Yoshida, Riichiro
Jiang, Chang-Jie
Takatsuji, Hiroshi
WRKY45 phosphorylation at threonine 266 acts negatively on WRKY45-dependent blast resistance in rice
title WRKY45 phosphorylation at threonine 266 acts negatively on WRKY45-dependent blast resistance in rice
title_full WRKY45 phosphorylation at threonine 266 acts negatively on WRKY45-dependent blast resistance in rice
title_fullStr WRKY45 phosphorylation at threonine 266 acts negatively on WRKY45-dependent blast resistance in rice
title_full_unstemmed WRKY45 phosphorylation at threonine 266 acts negatively on WRKY45-dependent blast resistance in rice
title_short WRKY45 phosphorylation at threonine 266 acts negatively on WRKY45-dependent blast resistance in rice
title_sort wrky45 phosphorylation at threonine 266 acts negatively on wrky45-dependent blast resistance in rice
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5616141/
https://www.ncbi.nlm.nih.gov/pubmed/28758876
http://dx.doi.org/10.1080/15592324.2017.1356968
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