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An immunocompetent mouse model of human glioblastoma
Orthotopic xenotransplantation studies represent the final stage in preclinical cancer research and could facilitate the implementation of precision medicine. To date, these xenografts have been tested in immunodeficient animals, but complete elimination of the adaptive immunity is a significant dra...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617407/ https://www.ncbi.nlm.nih.gov/pubmed/28977847 http://dx.doi.org/10.18632/oncotarget.17851 |
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author | Semenkow, Samantha Li, Shen Kahlert, Ulf D. Raabe, Eric H. Xu, Jiadi Arnold, Antje Janowski, Miroslaw Oh, Byoung Chol Brandacher, Gerald Bulte, Jeff W.M. Eberhart, Charles G. Walczak, Piotr |
author_facet | Semenkow, Samantha Li, Shen Kahlert, Ulf D. Raabe, Eric H. Xu, Jiadi Arnold, Antje Janowski, Miroslaw Oh, Byoung Chol Brandacher, Gerald Bulte, Jeff W.M. Eberhart, Charles G. Walczak, Piotr |
author_sort | Semenkow, Samantha |
collection | PubMed |
description | Orthotopic xenotransplantation studies represent the final stage in preclinical cancer research and could facilitate the implementation of precision medicine. To date, these xenografts have been tested in immunodeficient animals, but complete elimination of the adaptive immunity is a significant drawback. We present a method of efficient human glioblastoma (GBM) cell engraftment in adult mice with intact immune systems, mediated by a transient blockade of T-cell co-stimulation. Compared to transplants grown in immunodeficient hosts, the resulting tumors more accurately resemble the clinical pathophysiology of patient GBMs, which are characterized by blood-brain-barrier leakage and strong neo-vascularization. We expect our method to have great utility for studying human tumor cell biology, particularly in the field of cancer immunotherapy and in studies on microenvironmental interactions. Given the straightforward approach, the method may also be applicable to other tumor types and additional model organisms. |
format | Online Article Text |
id | pubmed-5617407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56174072017-10-03 An immunocompetent mouse model of human glioblastoma Semenkow, Samantha Li, Shen Kahlert, Ulf D. Raabe, Eric H. Xu, Jiadi Arnold, Antje Janowski, Miroslaw Oh, Byoung Chol Brandacher, Gerald Bulte, Jeff W.M. Eberhart, Charles G. Walczak, Piotr Oncotarget Research Paper Orthotopic xenotransplantation studies represent the final stage in preclinical cancer research and could facilitate the implementation of precision medicine. To date, these xenografts have been tested in immunodeficient animals, but complete elimination of the adaptive immunity is a significant drawback. We present a method of efficient human glioblastoma (GBM) cell engraftment in adult mice with intact immune systems, mediated by a transient blockade of T-cell co-stimulation. Compared to transplants grown in immunodeficient hosts, the resulting tumors more accurately resemble the clinical pathophysiology of patient GBMs, which are characterized by blood-brain-barrier leakage and strong neo-vascularization. We expect our method to have great utility for studying human tumor cell biology, particularly in the field of cancer immunotherapy and in studies on microenvironmental interactions. Given the straightforward approach, the method may also be applicable to other tumor types and additional model organisms. Impact Journals LLC 2017-05-15 /pmc/articles/PMC5617407/ /pubmed/28977847 http://dx.doi.org/10.18632/oncotarget.17851 Text en Copyright: © 2017 Semenkow et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Semenkow, Samantha Li, Shen Kahlert, Ulf D. Raabe, Eric H. Xu, Jiadi Arnold, Antje Janowski, Miroslaw Oh, Byoung Chol Brandacher, Gerald Bulte, Jeff W.M. Eberhart, Charles G. Walczak, Piotr An immunocompetent mouse model of human glioblastoma |
title | An immunocompetent mouse model of human glioblastoma |
title_full | An immunocompetent mouse model of human glioblastoma |
title_fullStr | An immunocompetent mouse model of human glioblastoma |
title_full_unstemmed | An immunocompetent mouse model of human glioblastoma |
title_short | An immunocompetent mouse model of human glioblastoma |
title_sort | immunocompetent mouse model of human glioblastoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617407/ https://www.ncbi.nlm.nih.gov/pubmed/28977847 http://dx.doi.org/10.18632/oncotarget.17851 |
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