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C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma
C-terminus kinesin motor KIFC1 is known for centrosome clustering in cancer cells with supernumerary centrosomes. KIFC1 crosslinks and glides on microtubules (MT) to assist normal bipolar spindle formation to avoid multi-polar cell division, which might be fatal. Testis cancer is the most common hum...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617430/ https://www.ncbi.nlm.nih.gov/pubmed/28977870 http://dx.doi.org/10.18632/oncotarget.18139 |
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author | Xiao, Yu-Xi Shen, Hao-Qing She, Zhen-Yu Sheng, Li Chen, Qian-Qian Chu, Yu-Lan Tan, Fu-Qing Yang, Wan-Xi |
author_facet | Xiao, Yu-Xi Shen, Hao-Qing She, Zhen-Yu Sheng, Li Chen, Qian-Qian Chu, Yu-Lan Tan, Fu-Qing Yang, Wan-Xi |
author_sort | Xiao, Yu-Xi |
collection | PubMed |
description | C-terminus kinesin motor KIFC1 is known for centrosome clustering in cancer cells with supernumerary centrosomes. KIFC1 crosslinks and glides on microtubules (MT) to assist normal bipolar spindle formation to avoid multi-polar cell division, which might be fatal. Testis cancer is the most common human cancer among young men. However, the gene expression profiles of testis cancer is still not complete and the expression of the C-terminus kinesin motor KIFC1 in testis cancer has not yet been examined. We found that KIFC1 is enriched in seminoma tissues in both mRNA level and protein level, and is specifically enriched in the cells that divide actively. Cell experiments showed that KIFC1 may be essential in cell division, but not essential in metastasis. Based on subcellular immuno-florescent staining results, we also described the localization of KIFC1 during cell cycle. By expressing ΔC-FLAG peptide in the cells, we found that the tail domain of KIFC1 might be essential for the dynamic disassociation of KIFC1, and the motor domain of KIFC1 might be essential for the degradation of KIFC1. Our work provides a new perspective for seminoma research. |
format | Online Article Text |
id | pubmed-5617430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56174302017-10-03 C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma Xiao, Yu-Xi Shen, Hao-Qing She, Zhen-Yu Sheng, Li Chen, Qian-Qian Chu, Yu-Lan Tan, Fu-Qing Yang, Wan-Xi Oncotarget Research Paper C-terminus kinesin motor KIFC1 is known for centrosome clustering in cancer cells with supernumerary centrosomes. KIFC1 crosslinks and glides on microtubules (MT) to assist normal bipolar spindle formation to avoid multi-polar cell division, which might be fatal. Testis cancer is the most common human cancer among young men. However, the gene expression profiles of testis cancer is still not complete and the expression of the C-terminus kinesin motor KIFC1 in testis cancer has not yet been examined. We found that KIFC1 is enriched in seminoma tissues in both mRNA level and protein level, and is specifically enriched in the cells that divide actively. Cell experiments showed that KIFC1 may be essential in cell division, but not essential in metastasis. Based on subcellular immuno-florescent staining results, we also described the localization of KIFC1 during cell cycle. By expressing ΔC-FLAG peptide in the cells, we found that the tail domain of KIFC1 might be essential for the dynamic disassociation of KIFC1, and the motor domain of KIFC1 might be essential for the degradation of KIFC1. Our work provides a new perspective for seminoma research. Impact Journals LLC 2017-05-24 /pmc/articles/PMC5617430/ /pubmed/28977870 http://dx.doi.org/10.18632/oncotarget.18139 Text en Copyright: © 2017 Xiao et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xiao, Yu-Xi Shen, Hao-Qing She, Zhen-Yu Sheng, Li Chen, Qian-Qian Chu, Yu-Lan Tan, Fu-Qing Yang, Wan-Xi C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma |
title | C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma |
title_full | C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma |
title_fullStr | C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma |
title_full_unstemmed | C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma |
title_short | C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma |
title_sort | c-terminal kinesin motor kifc1 participates in facilitating proper cell division of human seminoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617430/ https://www.ncbi.nlm.nih.gov/pubmed/28977870 http://dx.doi.org/10.18632/oncotarget.18139 |
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