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C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma

C-terminus kinesin motor KIFC1 is known for centrosome clustering in cancer cells with supernumerary centrosomes. KIFC1 crosslinks and glides on microtubules (MT) to assist normal bipolar spindle formation to avoid multi-polar cell division, which might be fatal. Testis cancer is the most common hum...

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Autores principales: Xiao, Yu-Xi, Shen, Hao-Qing, She, Zhen-Yu, Sheng, Li, Chen, Qian-Qian, Chu, Yu-Lan, Tan, Fu-Qing, Yang, Wan-Xi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617430/
https://www.ncbi.nlm.nih.gov/pubmed/28977870
http://dx.doi.org/10.18632/oncotarget.18139
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author Xiao, Yu-Xi
Shen, Hao-Qing
She, Zhen-Yu
Sheng, Li
Chen, Qian-Qian
Chu, Yu-Lan
Tan, Fu-Qing
Yang, Wan-Xi
author_facet Xiao, Yu-Xi
Shen, Hao-Qing
She, Zhen-Yu
Sheng, Li
Chen, Qian-Qian
Chu, Yu-Lan
Tan, Fu-Qing
Yang, Wan-Xi
author_sort Xiao, Yu-Xi
collection PubMed
description C-terminus kinesin motor KIFC1 is known for centrosome clustering in cancer cells with supernumerary centrosomes. KIFC1 crosslinks and glides on microtubules (MT) to assist normal bipolar spindle formation to avoid multi-polar cell division, which might be fatal. Testis cancer is the most common human cancer among young men. However, the gene expression profiles of testis cancer is still not complete and the expression of the C-terminus kinesin motor KIFC1 in testis cancer has not yet been examined. We found that KIFC1 is enriched in seminoma tissues in both mRNA level and protein level, and is specifically enriched in the cells that divide actively. Cell experiments showed that KIFC1 may be essential in cell division, but not essential in metastasis. Based on subcellular immuno-florescent staining results, we also described the localization of KIFC1 during cell cycle. By expressing ΔC-FLAG peptide in the cells, we found that the tail domain of KIFC1 might be essential for the dynamic disassociation of KIFC1, and the motor domain of KIFC1 might be essential for the degradation of KIFC1. Our work provides a new perspective for seminoma research.
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spelling pubmed-56174302017-10-03 C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma Xiao, Yu-Xi Shen, Hao-Qing She, Zhen-Yu Sheng, Li Chen, Qian-Qian Chu, Yu-Lan Tan, Fu-Qing Yang, Wan-Xi Oncotarget Research Paper C-terminus kinesin motor KIFC1 is known for centrosome clustering in cancer cells with supernumerary centrosomes. KIFC1 crosslinks and glides on microtubules (MT) to assist normal bipolar spindle formation to avoid multi-polar cell division, which might be fatal. Testis cancer is the most common human cancer among young men. However, the gene expression profiles of testis cancer is still not complete and the expression of the C-terminus kinesin motor KIFC1 in testis cancer has not yet been examined. We found that KIFC1 is enriched in seminoma tissues in both mRNA level and protein level, and is specifically enriched in the cells that divide actively. Cell experiments showed that KIFC1 may be essential in cell division, but not essential in metastasis. Based on subcellular immuno-florescent staining results, we also described the localization of KIFC1 during cell cycle. By expressing ΔC-FLAG peptide in the cells, we found that the tail domain of KIFC1 might be essential for the dynamic disassociation of KIFC1, and the motor domain of KIFC1 might be essential for the degradation of KIFC1. Our work provides a new perspective for seminoma research. Impact Journals LLC 2017-05-24 /pmc/articles/PMC5617430/ /pubmed/28977870 http://dx.doi.org/10.18632/oncotarget.18139 Text en Copyright: © 2017 Xiao et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Xiao, Yu-Xi
Shen, Hao-Qing
She, Zhen-Yu
Sheng, Li
Chen, Qian-Qian
Chu, Yu-Lan
Tan, Fu-Qing
Yang, Wan-Xi
C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma
title C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma
title_full C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma
title_fullStr C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma
title_full_unstemmed C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma
title_short C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma
title_sort c-terminal kinesin motor kifc1 participates in facilitating proper cell division of human seminoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617430/
https://www.ncbi.nlm.nih.gov/pubmed/28977870
http://dx.doi.org/10.18632/oncotarget.18139
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