Cargando…

Periostin promotes epithelial-mesenchymal transition via the MAPK/miR-381 axis in lung cancer

Periostin (POSTN, PN, or osteoblast-specific factor OSF-2) is a multifunctional cytokine that signals between the cell and the extracellular matrix. Periostin plays an important role in tumor development and is involved in carcinoma cell epithelial-mesenchymal transition (EMT), whereby mature epithe...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Wei-Wei, Chen, Po-Chun, Chen, Jun-Ming, Wu, Yue-Ming, Liu, Po-Yi, Lu, Chih-Hao, Lin, Yu-Feng, Tang, Chih-Hsin, Chao, Chia-Chia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617502/
https://www.ncbi.nlm.nih.gov/pubmed/28977942
http://dx.doi.org/10.18632/oncotarget.19273
_version_ 1783267002482163712
author Hu, Wei-Wei
Chen, Po-Chun
Chen, Jun-Ming
Wu, Yue-Ming
Liu, Po-Yi
Lu, Chih-Hao
Lin, Yu-Feng
Tang, Chih-Hsin
Chao, Chia-Chia
author_facet Hu, Wei-Wei
Chen, Po-Chun
Chen, Jun-Ming
Wu, Yue-Ming
Liu, Po-Yi
Lu, Chih-Hao
Lin, Yu-Feng
Tang, Chih-Hsin
Chao, Chia-Chia
author_sort Hu, Wei-Wei
collection PubMed
description Periostin (POSTN, PN, or osteoblast-specific factor OSF-2) is a multifunctional cytokine that signals between the cell and the extracellular matrix. Periostin plays an important role in tumor development and is involved in carcinoma cell epithelial-mesenchymal transition (EMT), whereby mature epithelial cells undergo phenotypic morphological changes and become invasive, motile cells. Here, we discuss the molecular mechanisms involved in periostin-induced promotion of EMT in lung cancer cells. Online TCGA datasets demonstrate the prognostic relevance of periostin in lung cancer; a higher periostin level correlates with poor overall survival. Similarly, our IHC results show that high periostin expression is positively correlated with the EMT markers Snail and Twist, as well as stage of lung cancer. We found that recombinant periostin induces the EMT phenotype in lung cancer cells through the p38/ERK pathway, while pretreatment with chemical inhibitors prevented periostin-induced EMT induction. Moreover, we found that periostin regulates EMT by repressing microRNA-381 (miR-381) expression, which targets both Snail and Twist. Using the miR-381 mimic, we dramatically reversed periostin-induced Snail and Twist expression. Furthermore, periostin knockdown dramatically affected EMT markers and cell migration potential. The role of periostin in lung cancer progression is elucidated by the in vivo mouse model. Our findings indicate that changes in periostin expression in lung cancer may serve as a therapeutic target for the treatment of lung cancer metastasis.
format Online
Article
Text
id pubmed-5617502
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-56175022017-10-03 Periostin promotes epithelial-mesenchymal transition via the MAPK/miR-381 axis in lung cancer Hu, Wei-Wei Chen, Po-Chun Chen, Jun-Ming Wu, Yue-Ming Liu, Po-Yi Lu, Chih-Hao Lin, Yu-Feng Tang, Chih-Hsin Chao, Chia-Chia Oncotarget Research Paper Periostin (POSTN, PN, or osteoblast-specific factor OSF-2) is a multifunctional cytokine that signals between the cell and the extracellular matrix. Periostin plays an important role in tumor development and is involved in carcinoma cell epithelial-mesenchymal transition (EMT), whereby mature epithelial cells undergo phenotypic morphological changes and become invasive, motile cells. Here, we discuss the molecular mechanisms involved in periostin-induced promotion of EMT in lung cancer cells. Online TCGA datasets demonstrate the prognostic relevance of periostin in lung cancer; a higher periostin level correlates with poor overall survival. Similarly, our IHC results show that high periostin expression is positively correlated with the EMT markers Snail and Twist, as well as stage of lung cancer. We found that recombinant periostin induces the EMT phenotype in lung cancer cells through the p38/ERK pathway, while pretreatment with chemical inhibitors prevented periostin-induced EMT induction. Moreover, we found that periostin regulates EMT by repressing microRNA-381 (miR-381) expression, which targets both Snail and Twist. Using the miR-381 mimic, we dramatically reversed periostin-induced Snail and Twist expression. Furthermore, periostin knockdown dramatically affected EMT markers and cell migration potential. The role of periostin in lung cancer progression is elucidated by the in vivo mouse model. Our findings indicate that changes in periostin expression in lung cancer may serve as a therapeutic target for the treatment of lung cancer metastasis. Impact Journals LLC 2017-07-15 /pmc/articles/PMC5617502/ /pubmed/28977942 http://dx.doi.org/10.18632/oncotarget.19273 Text en Copyright: © 2017 Hu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Hu, Wei-Wei
Chen, Po-Chun
Chen, Jun-Ming
Wu, Yue-Ming
Liu, Po-Yi
Lu, Chih-Hao
Lin, Yu-Feng
Tang, Chih-Hsin
Chao, Chia-Chia
Periostin promotes epithelial-mesenchymal transition via the MAPK/miR-381 axis in lung cancer
title Periostin promotes epithelial-mesenchymal transition via the MAPK/miR-381 axis in lung cancer
title_full Periostin promotes epithelial-mesenchymal transition via the MAPK/miR-381 axis in lung cancer
title_fullStr Periostin promotes epithelial-mesenchymal transition via the MAPK/miR-381 axis in lung cancer
title_full_unstemmed Periostin promotes epithelial-mesenchymal transition via the MAPK/miR-381 axis in lung cancer
title_short Periostin promotes epithelial-mesenchymal transition via the MAPK/miR-381 axis in lung cancer
title_sort periostin promotes epithelial-mesenchymal transition via the mapk/mir-381 axis in lung cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617502/
https://www.ncbi.nlm.nih.gov/pubmed/28977942
http://dx.doi.org/10.18632/oncotarget.19273
work_keys_str_mv AT huweiwei periostinpromotesepithelialmesenchymaltransitionviathemapkmir381axisinlungcancer
AT chenpochun periostinpromotesepithelialmesenchymaltransitionviathemapkmir381axisinlungcancer
AT chenjunming periostinpromotesepithelialmesenchymaltransitionviathemapkmir381axisinlungcancer
AT wuyueming periostinpromotesepithelialmesenchymaltransitionviathemapkmir381axisinlungcancer
AT liupoyi periostinpromotesepithelialmesenchymaltransitionviathemapkmir381axisinlungcancer
AT luchihhao periostinpromotesepithelialmesenchymaltransitionviathemapkmir381axisinlungcancer
AT linyufeng periostinpromotesepithelialmesenchymaltransitionviathemapkmir381axisinlungcancer
AT tangchihhsin periostinpromotesepithelialmesenchymaltransitionviathemapkmir381axisinlungcancer
AT chaochiachia periostinpromotesepithelialmesenchymaltransitionviathemapkmir381axisinlungcancer