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Optimizing the fragment complementation of APEX2 for detection of specific protein-protein interactions in live cells
Dynamic protein-protein interactions (PPIs) play crucial roles in cell physiological processes. The protein-fragment complementation (PFC) assay has been developed as a powerful approach for the detection of PPIs, but its potential for identifying protein interacting regions is not optimized. Recent...
Autores principales: | Xue, Miaomiao, Hou, Junjie, Wang, Linlin, Cheng, Dongwan, Lu, Jingze, Zheng, Li, Xu, Tao |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617831/ https://www.ncbi.nlm.nih.gov/pubmed/28955036 http://dx.doi.org/10.1038/s41598-017-12365-9 |
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