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Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides

Seven analogs of the natural, α-helix peptides IsCT1 and IsCT2—found in the venom of scorpion Opithancatus Madagascariensis—have been synthesized and tested to compare their antibacterial and hemolytic activity against natural peptides. In general, results show that increasing hydrophobicity by subs...

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Autores principales: de la Salud Bea, Roberto, Petraglia, Adam F., Ascuitto, Michael R., Buck, Quentin M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617977/
https://www.ncbi.nlm.nih.gov/pubmed/28657596
http://dx.doi.org/10.3390/antibiotics6030013
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author de la Salud Bea, Roberto
Petraglia, Adam F.
Ascuitto, Michael R.
Buck, Quentin M.
author_facet de la Salud Bea, Roberto
Petraglia, Adam F.
Ascuitto, Michael R.
Buck, Quentin M.
author_sort de la Salud Bea, Roberto
collection PubMed
description Seven analogs of the natural, α-helix peptides IsCT1 and IsCT2—found in the venom of scorpion Opithancatus Madagascariensis—have been synthesized and tested to compare their antibacterial and hemolytic activity against natural peptides. In general, results show that increasing hydrophobicity by substituting positions 5 and 9 of the sequences with alanine, valine, and leucine, enhances antibacterial activity. However, this also increases hemolytic activity. The analog with an increased net positive charge from +1 to +3 produces moderate bacterial growth inhibition but also has high hemolytic activity. On the other hand, the analog with a negative net charge (−1) has low antibacterial properties but also no cytotoxicity under the tested conditions, a similar result was found for five of the seven studied analogs.
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spelling pubmed-56179772017-09-29 Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides de la Salud Bea, Roberto Petraglia, Adam F. Ascuitto, Michael R. Buck, Quentin M. Antibiotics (Basel) Article Seven analogs of the natural, α-helix peptides IsCT1 and IsCT2—found in the venom of scorpion Opithancatus Madagascariensis—have been synthesized and tested to compare their antibacterial and hemolytic activity against natural peptides. In general, results show that increasing hydrophobicity by substituting positions 5 and 9 of the sequences with alanine, valine, and leucine, enhances antibacterial activity. However, this also increases hemolytic activity. The analog with an increased net positive charge from +1 to +3 produces moderate bacterial growth inhibition but also has high hemolytic activity. On the other hand, the analog with a negative net charge (−1) has low antibacterial properties but also no cytotoxicity under the tested conditions, a similar result was found for five of the seven studied analogs. MDPI 2017-06-28 /pmc/articles/PMC5617977/ /pubmed/28657596 http://dx.doi.org/10.3390/antibiotics6030013 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de la Salud Bea, Roberto
Petraglia, Adam F.
Ascuitto, Michael R.
Buck, Quentin M.
Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides
title Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides
title_full Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides
title_fullStr Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides
title_full_unstemmed Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides
title_short Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides
title_sort antibacterial activity and toxicity of analogs of scorpion venom isct peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617977/
https://www.ncbi.nlm.nih.gov/pubmed/28657596
http://dx.doi.org/10.3390/antibiotics6030013
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