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Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides
Seven analogs of the natural, α-helix peptides IsCT1 and IsCT2—found in the venom of scorpion Opithancatus Madagascariensis—have been synthesized and tested to compare their antibacterial and hemolytic activity against natural peptides. In general, results show that increasing hydrophobicity by subs...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617977/ https://www.ncbi.nlm.nih.gov/pubmed/28657596 http://dx.doi.org/10.3390/antibiotics6030013 |
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author | de la Salud Bea, Roberto Petraglia, Adam F. Ascuitto, Michael R. Buck, Quentin M. |
author_facet | de la Salud Bea, Roberto Petraglia, Adam F. Ascuitto, Michael R. Buck, Quentin M. |
author_sort | de la Salud Bea, Roberto |
collection | PubMed |
description | Seven analogs of the natural, α-helix peptides IsCT1 and IsCT2—found in the venom of scorpion Opithancatus Madagascariensis—have been synthesized and tested to compare their antibacterial and hemolytic activity against natural peptides. In general, results show that increasing hydrophobicity by substituting positions 5 and 9 of the sequences with alanine, valine, and leucine, enhances antibacterial activity. However, this also increases hemolytic activity. The analog with an increased net positive charge from +1 to +3 produces moderate bacterial growth inhibition but also has high hemolytic activity. On the other hand, the analog with a negative net charge (−1) has low antibacterial properties but also no cytotoxicity under the tested conditions, a similar result was found for five of the seven studied analogs. |
format | Online Article Text |
id | pubmed-5617977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56179772017-09-29 Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides de la Salud Bea, Roberto Petraglia, Adam F. Ascuitto, Michael R. Buck, Quentin M. Antibiotics (Basel) Article Seven analogs of the natural, α-helix peptides IsCT1 and IsCT2—found in the venom of scorpion Opithancatus Madagascariensis—have been synthesized and tested to compare their antibacterial and hemolytic activity against natural peptides. In general, results show that increasing hydrophobicity by substituting positions 5 and 9 of the sequences with alanine, valine, and leucine, enhances antibacterial activity. However, this also increases hemolytic activity. The analog with an increased net positive charge from +1 to +3 produces moderate bacterial growth inhibition but also has high hemolytic activity. On the other hand, the analog with a negative net charge (−1) has low antibacterial properties but also no cytotoxicity under the tested conditions, a similar result was found for five of the seven studied analogs. MDPI 2017-06-28 /pmc/articles/PMC5617977/ /pubmed/28657596 http://dx.doi.org/10.3390/antibiotics6030013 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de la Salud Bea, Roberto Petraglia, Adam F. Ascuitto, Michael R. Buck, Quentin M. Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides |
title | Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides |
title_full | Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides |
title_fullStr | Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides |
title_full_unstemmed | Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides |
title_short | Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides |
title_sort | antibacterial activity and toxicity of analogs of scorpion venom isct peptides |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617977/ https://www.ncbi.nlm.nih.gov/pubmed/28657596 http://dx.doi.org/10.3390/antibiotics6030013 |
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