Cargando…

A Novel Surface Plasmon Resonance Biosensor for the Rapid Detection of Botulinum Neurotoxins

Botulinum neurotoxins (BoNTs) are Category A agents on the NIAID (National Institute of Allergy and Infectious Diseases) priority pathogen list owing to their extreme toxicity and the relative ease of production. These deadly toxins, in minute quantities (estimated human i.v. lethal dose LD(50) of 1...

Descripción completa

Detalles Bibliográficos
Autores principales: Patel, Kruti, Halevi, Shmuel, Melman, Paul, Schwartz, John, Cai, Shuowei, Singh, Bal Ram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618038/
https://www.ncbi.nlm.nih.gov/pubmed/28783115
http://dx.doi.org/10.3390/bios7030032
_version_ 1783267097835470848
author Patel, Kruti
Halevi, Shmuel
Melman, Paul
Schwartz, John
Cai, Shuowei
Singh, Bal Ram
author_facet Patel, Kruti
Halevi, Shmuel
Melman, Paul
Schwartz, John
Cai, Shuowei
Singh, Bal Ram
author_sort Patel, Kruti
collection PubMed
description Botulinum neurotoxins (BoNTs) are Category A agents on the NIAID (National Institute of Allergy and Infectious Diseases) priority pathogen list owing to their extreme toxicity and the relative ease of production. These deadly toxins, in minute quantities (estimated human i.v. lethal dose LD(50) of 1–2 ng/kg body weight), cause fatal flaccid paralysis by blocking neurotransmitter release. The current gold standard detection method, the mouse-bioassay, often takes days to confirm botulism. Furthermore, there are no effective antidotes known to reverse the symptoms of botulism, and as a result, patients with severe botulism often require meticulous care during the prolonged paralytic illness. To combat potential bio-terrorism incidents of botulinum neurotoxins, their rapid detection is paramount. Surface plasmon resonance (SPR) is a very sensitive technique to examine bio-molecular interactions. The label-free, real-time analysis, with high sensitivity and low sample consumption makes this technology particularly suitable for detection of the toxin. In this study, we demonstrated the feasibility in an assay with a newly designed SPR instrument for the rapid detection of botulinum neurotoxins. The LOD (limit of detection) of the Newton Photonics (NP) SPR based assay is 6.76 pg/mL for Botulinum Neurotoxin type A Light Chain (BoNT/A LC). We established that the detection sensitivity of the system is comparable to the traditional mouse LD(50) bioassay in BoNT/A using this SPR technology.
format Online
Article
Text
id pubmed-5618038
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-56180382017-09-29 A Novel Surface Plasmon Resonance Biosensor for the Rapid Detection of Botulinum Neurotoxins Patel, Kruti Halevi, Shmuel Melman, Paul Schwartz, John Cai, Shuowei Singh, Bal Ram Biosensors (Basel) Article Botulinum neurotoxins (BoNTs) are Category A agents on the NIAID (National Institute of Allergy and Infectious Diseases) priority pathogen list owing to their extreme toxicity and the relative ease of production. These deadly toxins, in minute quantities (estimated human i.v. lethal dose LD(50) of 1–2 ng/kg body weight), cause fatal flaccid paralysis by blocking neurotransmitter release. The current gold standard detection method, the mouse-bioassay, often takes days to confirm botulism. Furthermore, there are no effective antidotes known to reverse the symptoms of botulism, and as a result, patients with severe botulism often require meticulous care during the prolonged paralytic illness. To combat potential bio-terrorism incidents of botulinum neurotoxins, their rapid detection is paramount. Surface plasmon resonance (SPR) is a very sensitive technique to examine bio-molecular interactions. The label-free, real-time analysis, with high sensitivity and low sample consumption makes this technology particularly suitable for detection of the toxin. In this study, we demonstrated the feasibility in an assay with a newly designed SPR instrument for the rapid detection of botulinum neurotoxins. The LOD (limit of detection) of the Newton Photonics (NP) SPR based assay is 6.76 pg/mL for Botulinum Neurotoxin type A Light Chain (BoNT/A LC). We established that the detection sensitivity of the system is comparable to the traditional mouse LD(50) bioassay in BoNT/A using this SPR technology. MDPI 2017-08-07 /pmc/articles/PMC5618038/ /pubmed/28783115 http://dx.doi.org/10.3390/bios7030032 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Patel, Kruti
Halevi, Shmuel
Melman, Paul
Schwartz, John
Cai, Shuowei
Singh, Bal Ram
A Novel Surface Plasmon Resonance Biosensor for the Rapid Detection of Botulinum Neurotoxins
title A Novel Surface Plasmon Resonance Biosensor for the Rapid Detection of Botulinum Neurotoxins
title_full A Novel Surface Plasmon Resonance Biosensor for the Rapid Detection of Botulinum Neurotoxins
title_fullStr A Novel Surface Plasmon Resonance Biosensor for the Rapid Detection of Botulinum Neurotoxins
title_full_unstemmed A Novel Surface Plasmon Resonance Biosensor for the Rapid Detection of Botulinum Neurotoxins
title_short A Novel Surface Plasmon Resonance Biosensor for the Rapid Detection of Botulinum Neurotoxins
title_sort novel surface plasmon resonance biosensor for the rapid detection of botulinum neurotoxins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618038/
https://www.ncbi.nlm.nih.gov/pubmed/28783115
http://dx.doi.org/10.3390/bios7030032
work_keys_str_mv AT patelkruti anovelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins
AT halevishmuel anovelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins
AT melmanpaul anovelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins
AT schwartzjohn anovelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins
AT caishuowei anovelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins
AT singhbalram anovelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins
AT patelkruti novelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins
AT halevishmuel novelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins
AT melmanpaul novelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins
AT schwartzjohn novelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins
AT caishuowei novelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins
AT singhbalram novelsurfaceplasmonresonancebiosensorfortherapiddetectionofbotulinumneurotoxins