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Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment

We report on the use of one-dimensional photonic crystals to detect clinically relevant concentrations of the cancer biomarker ERBB2 in cell lysates. Overexpression of the ERBB2 protein is associated with aggressive breast cancer subtypes. To detect soluble ERBB2, we developed an optical set-up whic...

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Detalles Bibliográficos
Autores principales: Sinibaldi, Alberto, Sampaoli, Camilla, Danz, Norbert, Munzert, Peter, Sonntag, Frank, Centola, Fabio, Occhicone, Agostino, Tremante, Elisa, Giacomini, Patrizio, Michelotti, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618039/
https://www.ncbi.nlm.nih.gov/pubmed/28817097
http://dx.doi.org/10.3390/bios7030033
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author Sinibaldi, Alberto
Sampaoli, Camilla
Danz, Norbert
Munzert, Peter
Sonntag, Frank
Centola, Fabio
Occhicone, Agostino
Tremante, Elisa
Giacomini, Patrizio
Michelotti, Francesco
author_facet Sinibaldi, Alberto
Sampaoli, Camilla
Danz, Norbert
Munzert, Peter
Sonntag, Frank
Centola, Fabio
Occhicone, Agostino
Tremante, Elisa
Giacomini, Patrizio
Michelotti, Francesco
author_sort Sinibaldi, Alberto
collection PubMed
description We report on the use of one-dimensional photonic crystals to detect clinically relevant concentrations of the cancer biomarker ERBB2 in cell lysates. Overexpression of the ERBB2 protein is associated with aggressive breast cancer subtypes. To detect soluble ERBB2, we developed an optical set-up which operates in both label-free and fluorescence modes. The detection approach makes use of a sandwich assay, in which the one-dimensional photonic crystals sustaining Bloch surface waves are modified with monoclonal antibodies, in order to guarantee high specificity during the biological recognition. We present the results of exemplary protein G based label-free assays in complex biological matrices, reaching an estimated limit of detection of 0.5 ng/mL. On-chip and chip-to-chip variability of the results is addressed too, providing repeatability rates. Moreover, results on fluorescence operation demonstrate the capability to perform high sensitive cancer biomarker assays reaching a resolution of 0.6 ng/mL, without protein G assistance. The resolution obtained in both modes meets international guidelines and recommendations (15 ng/mL) for ERBB2 quantification assays, providing an alternative tool to phenotype and diagnose molecular cancer subtypes.
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spelling pubmed-56180392017-09-29 Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment Sinibaldi, Alberto Sampaoli, Camilla Danz, Norbert Munzert, Peter Sonntag, Frank Centola, Fabio Occhicone, Agostino Tremante, Elisa Giacomini, Patrizio Michelotti, Francesco Biosensors (Basel) Article We report on the use of one-dimensional photonic crystals to detect clinically relevant concentrations of the cancer biomarker ERBB2 in cell lysates. Overexpression of the ERBB2 protein is associated with aggressive breast cancer subtypes. To detect soluble ERBB2, we developed an optical set-up which operates in both label-free and fluorescence modes. The detection approach makes use of a sandwich assay, in which the one-dimensional photonic crystals sustaining Bloch surface waves are modified with monoclonal antibodies, in order to guarantee high specificity during the biological recognition. We present the results of exemplary protein G based label-free assays in complex biological matrices, reaching an estimated limit of detection of 0.5 ng/mL. On-chip and chip-to-chip variability of the results is addressed too, providing repeatability rates. Moreover, results on fluorescence operation demonstrate the capability to perform high sensitive cancer biomarker assays reaching a resolution of 0.6 ng/mL, without protein G assistance. The resolution obtained in both modes meets international guidelines and recommendations (15 ng/mL) for ERBB2 quantification assays, providing an alternative tool to phenotype and diagnose molecular cancer subtypes. MDPI 2017-08-17 /pmc/articles/PMC5618039/ /pubmed/28817097 http://dx.doi.org/10.3390/bios7030033 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sinibaldi, Alberto
Sampaoli, Camilla
Danz, Norbert
Munzert, Peter
Sonntag, Frank
Centola, Fabio
Occhicone, Agostino
Tremante, Elisa
Giacomini, Patrizio
Michelotti, Francesco
Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment
title Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment
title_full Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment
title_fullStr Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment
title_full_unstemmed Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment
title_short Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment
title_sort bloch surface waves biosensors for high sensitivity detection of soluble erbb2 in a complex biological environment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618039/
https://www.ncbi.nlm.nih.gov/pubmed/28817097
http://dx.doi.org/10.3390/bios7030033
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