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Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques
Biomimetic planar artificial membranes have been widely studied due to their multiple applications in several research fields. Their humectation and thermal response are crucial for reaching stability; these characteristics are related to the molecular organization inside the bilayer, which is affec...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618040/ https://www.ncbi.nlm.nih.gov/pubmed/28820461 http://dx.doi.org/10.3390/bios7030034 |
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author | González-Henríquez, Carmen M. Villegas-Opazo, Vanessa A. Sagredo-Oyarce, Dallits H. Sarabia-Vallejos, Mauricio A. Terraza, Claudio A. |
author_facet | González-Henríquez, Carmen M. Villegas-Opazo, Vanessa A. Sagredo-Oyarce, Dallits H. Sarabia-Vallejos, Mauricio A. Terraza, Claudio A. |
author_sort | González-Henríquez, Carmen M. |
collection | PubMed |
description | Biomimetic planar artificial membranes have been widely studied due to their multiple applications in several research fields. Their humectation and thermal response are crucial for reaching stability; these characteristics are related to the molecular organization inside the bilayer, which is affected by the aliphatic chain length, saturations, and molecule polarity, among others. Bilayer stability becomes a fundamental factor when technological devices are developed—like biosensors—based on those systems. Thermal studies were performed for different types of phosphatidylcholine (PC) molecules: two pure PC bilayers and four binary PC mixtures. These analyses were carried out through the detection of slight changes in their optical and structural parameters via Ellipsometry and Surface Plasmon Resonance (SPR) techniques. Phospholipid bilayers were prepared by Langmuir-Blodgett technique and deposited over a hydrophilic silicon wafer. Their molecular inclination degree, mobility, and stability of the different phases were detected and analyzed through bilayer thickness changes and their optical phase-amplitude response. Results show that certain binary lipid mixtures—with differences in its aliphatic chain length—present a co-existence of two thermal responses due to non-ideal mixing. |
format | Online Article Text |
id | pubmed-5618040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56180402017-09-29 Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques González-Henríquez, Carmen M. Villegas-Opazo, Vanessa A. Sagredo-Oyarce, Dallits H. Sarabia-Vallejos, Mauricio A. Terraza, Claudio A. Biosensors (Basel) Article Biomimetic planar artificial membranes have been widely studied due to their multiple applications in several research fields. Their humectation and thermal response are crucial for reaching stability; these characteristics are related to the molecular organization inside the bilayer, which is affected by the aliphatic chain length, saturations, and molecule polarity, among others. Bilayer stability becomes a fundamental factor when technological devices are developed—like biosensors—based on those systems. Thermal studies were performed for different types of phosphatidylcholine (PC) molecules: two pure PC bilayers and four binary PC mixtures. These analyses were carried out through the detection of slight changes in their optical and structural parameters via Ellipsometry and Surface Plasmon Resonance (SPR) techniques. Phospholipid bilayers were prepared by Langmuir-Blodgett technique and deposited over a hydrophilic silicon wafer. Their molecular inclination degree, mobility, and stability of the different phases were detected and analyzed through bilayer thickness changes and their optical phase-amplitude response. Results show that certain binary lipid mixtures—with differences in its aliphatic chain length—present a co-existence of two thermal responses due to non-ideal mixing. MDPI 2017-08-18 /pmc/articles/PMC5618040/ /pubmed/28820461 http://dx.doi.org/10.3390/bios7030034 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article González-Henríquez, Carmen M. Villegas-Opazo, Vanessa A. Sagredo-Oyarce, Dallits H. Sarabia-Vallejos, Mauricio A. Terraza, Claudio A. Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques |
title | Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques |
title_full | Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques |
title_fullStr | Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques |
title_full_unstemmed | Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques |
title_short | Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques |
title_sort | thermal response analysis of phospholipid bilayers using ellipsometric techniques |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618040/ https://www.ncbi.nlm.nih.gov/pubmed/28820461 http://dx.doi.org/10.3390/bios7030034 |
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