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Attenuation of Red Blood Cell Storage Lesions with Vitamin C

Stored red blood cells (RBCs) undergo oxidative stress that induces deleterious metabolic, structural, biochemical, and molecular changes collectively referred to as “storage lesions”. We hypothesized that vitamin C (VitC, reduced or oxidized) would reduce red cell storage lesions, thus prolonging t...

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Autores principales: Sanford, Kimberly, Fisher, Bernard J., Fowler, Evan, Fowler, Alpha A., Natarajan, Ramesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618083/
https://www.ncbi.nlm.nih.gov/pubmed/28704937
http://dx.doi.org/10.3390/antiox6030055
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author Sanford, Kimberly
Fisher, Bernard J.
Fowler, Evan
Fowler, Alpha A.
Natarajan, Ramesh
author_facet Sanford, Kimberly
Fisher, Bernard J.
Fowler, Evan
Fowler, Alpha A.
Natarajan, Ramesh
author_sort Sanford, Kimberly
collection PubMed
description Stored red blood cells (RBCs) undergo oxidative stress that induces deleterious metabolic, structural, biochemical, and molecular changes collectively referred to as “storage lesions”. We hypothesized that vitamin C (VitC, reduced or oxidized) would reduce red cell storage lesions, thus prolonging their storage duration. Whole-blood-derived, leuko-reduced, SAGM (saline-adenine-glucose-mannitol)-preserved RBC concentrates were equally divided into four pediatric storage bags and the following additions made: (1) saline (saline); (2) 0.3 mmol/L reduced VitC (Lo VitC); (3) 3 mmol/L reduced VitC (Hi VitC); or (4) 0.3 mmol/L oxidized VitC (dehydroascorbic acid, DHA) as final concentrations. Biochemical and rheological parameters were serially assessed at baseline (prior to supplementation) and Days 7, 21, 42, and 56 for RBC VitC concentration, pH, osmotic fragility by mechanical fragility index, and percent hemolysis, LDH release, glutathione depletion, RBC membrane integrity by scanning electron microscopy, and Western blot for β-spectrin. VitC exposure (reduced and oxidized) significantly increased RBC antioxidant status with varying dynamics and produced trends in reduction in osmotic fragility and increases in membrane integrity. Conclusion: VitC partially protects RBC from oxidative changes during storage. Combining VitC with other antioxidants has the potential to improve long-term storage of RBC.
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spelling pubmed-56180832017-09-29 Attenuation of Red Blood Cell Storage Lesions with Vitamin C Sanford, Kimberly Fisher, Bernard J. Fowler, Evan Fowler, Alpha A. Natarajan, Ramesh Antioxidants (Basel) Article Stored red blood cells (RBCs) undergo oxidative stress that induces deleterious metabolic, structural, biochemical, and molecular changes collectively referred to as “storage lesions”. We hypothesized that vitamin C (VitC, reduced or oxidized) would reduce red cell storage lesions, thus prolonging their storage duration. Whole-blood-derived, leuko-reduced, SAGM (saline-adenine-glucose-mannitol)-preserved RBC concentrates were equally divided into four pediatric storage bags and the following additions made: (1) saline (saline); (2) 0.3 mmol/L reduced VitC (Lo VitC); (3) 3 mmol/L reduced VitC (Hi VitC); or (4) 0.3 mmol/L oxidized VitC (dehydroascorbic acid, DHA) as final concentrations. Biochemical and rheological parameters were serially assessed at baseline (prior to supplementation) and Days 7, 21, 42, and 56 for RBC VitC concentration, pH, osmotic fragility by mechanical fragility index, and percent hemolysis, LDH release, glutathione depletion, RBC membrane integrity by scanning electron microscopy, and Western blot for β-spectrin. VitC exposure (reduced and oxidized) significantly increased RBC antioxidant status with varying dynamics and produced trends in reduction in osmotic fragility and increases in membrane integrity. Conclusion: VitC partially protects RBC from oxidative changes during storage. Combining VitC with other antioxidants has the potential to improve long-term storage of RBC. MDPI 2017-07-12 /pmc/articles/PMC5618083/ /pubmed/28704937 http://dx.doi.org/10.3390/antiox6030055 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sanford, Kimberly
Fisher, Bernard J.
Fowler, Evan
Fowler, Alpha A.
Natarajan, Ramesh
Attenuation of Red Blood Cell Storage Lesions with Vitamin C
title Attenuation of Red Blood Cell Storage Lesions with Vitamin C
title_full Attenuation of Red Blood Cell Storage Lesions with Vitamin C
title_fullStr Attenuation of Red Blood Cell Storage Lesions with Vitamin C
title_full_unstemmed Attenuation of Red Blood Cell Storage Lesions with Vitamin C
title_short Attenuation of Red Blood Cell Storage Lesions with Vitamin C
title_sort attenuation of red blood cell storage lesions with vitamin c
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618083/
https://www.ncbi.nlm.nih.gov/pubmed/28704937
http://dx.doi.org/10.3390/antiox6030055
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