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Moderate blast exposure alters gene expression and levels of amyloid precursor protein

OBJECTIVE: To explore gene expression after moderate blast exposure (vs baseline) and proteomic changes after moderate- (vs low-) blast exposure. METHODS: Military personnel (N = 69) donated blood for quantification of protein level, and peak pressure exposures were detected by helmet sensors before...

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Autores principales: Gill, Jessica, Cashion, Ann, Osier, Nicole, Arcurio, Lindsay, Motamedi, Vida, Dell, Kristine C., Carr, Walter, Kim, Hyung-Suk, Yun, Sijung, Walker, Peter, Ahlers, Stephen, LoPresti, Matthew, Yarnell, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618107/
https://www.ncbi.nlm.nih.gov/pubmed/28975156
http://dx.doi.org/10.1212/NXG.0000000000000186
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author Gill, Jessica
Cashion, Ann
Osier, Nicole
Arcurio, Lindsay
Motamedi, Vida
Dell, Kristine C.
Carr, Walter
Kim, Hyung-Suk
Yun, Sijung
Walker, Peter
Ahlers, Stephen
LoPresti, Matthew
Yarnell, Angela
author_facet Gill, Jessica
Cashion, Ann
Osier, Nicole
Arcurio, Lindsay
Motamedi, Vida
Dell, Kristine C.
Carr, Walter
Kim, Hyung-Suk
Yun, Sijung
Walker, Peter
Ahlers, Stephen
LoPresti, Matthew
Yarnell, Angela
author_sort Gill, Jessica
collection PubMed
description OBJECTIVE: To explore gene expression after moderate blast exposure (vs baseline) and proteomic changes after moderate- (vs low-) blast exposure. METHODS: Military personnel (N = 69) donated blood for quantification of protein level, and peak pressure exposures were detected by helmet sensors before and during a blast training program (10 days total). On day 7, some participants (n = 29) sustained a moderate blast (mean peak pressure = 7.9 psi) and were matched to participants with no/low-blast exposure during the training (n = 40). PAXgene tubes were collected from one training site at baseline and day 10; RNA-sequencing day 10 expression was compared with each participant's own baseline samples to identify genes and pathways differentially expressed in moderate blast-exposed participants. Changes in amyloid precursor protein (APP) from baseline to the day of blast and following 2 days were evaluated. Symptoms were assessed using a self-reported form. RESULTS: We identified 1,803 differentially expressed genes after moderate blast exposure; the most altered network was APP. Significantly reduced levels of peripheral APP were detected the day after the moderate blast exposure and the following day. Protein concentrations correlated with the magnitude of the moderate blast exposure on days 8 and 9. APP concentrations returned to baseline levels 3 days following the blast, likely due to increases in the genetic expression of APP. Onset of concentration problems and headaches occurred after moderate blast. CONCLUSIONS: Moderate blast exposure results in a signature biological profile that includes acute APP reductions, followed by genetic expression increases and normalization of APP levels; these changes likely influence neuronal recovery.
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spelling pubmed-56181072017-10-03 Moderate blast exposure alters gene expression and levels of amyloid precursor protein Gill, Jessica Cashion, Ann Osier, Nicole Arcurio, Lindsay Motamedi, Vida Dell, Kristine C. Carr, Walter Kim, Hyung-Suk Yun, Sijung Walker, Peter Ahlers, Stephen LoPresti, Matthew Yarnell, Angela Neurol Genet Article OBJECTIVE: To explore gene expression after moderate blast exposure (vs baseline) and proteomic changes after moderate- (vs low-) blast exposure. METHODS: Military personnel (N = 69) donated blood for quantification of protein level, and peak pressure exposures were detected by helmet sensors before and during a blast training program (10 days total). On day 7, some participants (n = 29) sustained a moderate blast (mean peak pressure = 7.9 psi) and were matched to participants with no/low-blast exposure during the training (n = 40). PAXgene tubes were collected from one training site at baseline and day 10; RNA-sequencing day 10 expression was compared with each participant's own baseline samples to identify genes and pathways differentially expressed in moderate blast-exposed participants. Changes in amyloid precursor protein (APP) from baseline to the day of blast and following 2 days were evaluated. Symptoms were assessed using a self-reported form. RESULTS: We identified 1,803 differentially expressed genes after moderate blast exposure; the most altered network was APP. Significantly reduced levels of peripheral APP were detected the day after the moderate blast exposure and the following day. Protein concentrations correlated with the magnitude of the moderate blast exposure on days 8 and 9. APP concentrations returned to baseline levels 3 days following the blast, likely due to increases in the genetic expression of APP. Onset of concentration problems and headaches occurred after moderate blast. CONCLUSIONS: Moderate blast exposure results in a signature biological profile that includes acute APP reductions, followed by genetic expression increases and normalization of APP levels; these changes likely influence neuronal recovery. Wolters Kluwer 2017-09-27 /pmc/articles/PMC5618107/ /pubmed/28975156 http://dx.doi.org/10.1212/NXG.0000000000000186 Text en Copyright © 2017 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
spellingShingle Article
Gill, Jessica
Cashion, Ann
Osier, Nicole
Arcurio, Lindsay
Motamedi, Vida
Dell, Kristine C.
Carr, Walter
Kim, Hyung-Suk
Yun, Sijung
Walker, Peter
Ahlers, Stephen
LoPresti, Matthew
Yarnell, Angela
Moderate blast exposure alters gene expression and levels of amyloid precursor protein
title Moderate blast exposure alters gene expression and levels of amyloid precursor protein
title_full Moderate blast exposure alters gene expression and levels of amyloid precursor protein
title_fullStr Moderate blast exposure alters gene expression and levels of amyloid precursor protein
title_full_unstemmed Moderate blast exposure alters gene expression and levels of amyloid precursor protein
title_short Moderate blast exposure alters gene expression and levels of amyloid precursor protein
title_sort moderate blast exposure alters gene expression and levels of amyloid precursor protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618107/
https://www.ncbi.nlm.nih.gov/pubmed/28975156
http://dx.doi.org/10.1212/NXG.0000000000000186
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