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Moderate blast exposure alters gene expression and levels of amyloid precursor protein
OBJECTIVE: To explore gene expression after moderate blast exposure (vs baseline) and proteomic changes after moderate- (vs low-) blast exposure. METHODS: Military personnel (N = 69) donated blood for quantification of protein level, and peak pressure exposures were detected by helmet sensors before...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618107/ https://www.ncbi.nlm.nih.gov/pubmed/28975156 http://dx.doi.org/10.1212/NXG.0000000000000186 |
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author | Gill, Jessica Cashion, Ann Osier, Nicole Arcurio, Lindsay Motamedi, Vida Dell, Kristine C. Carr, Walter Kim, Hyung-Suk Yun, Sijung Walker, Peter Ahlers, Stephen LoPresti, Matthew Yarnell, Angela |
author_facet | Gill, Jessica Cashion, Ann Osier, Nicole Arcurio, Lindsay Motamedi, Vida Dell, Kristine C. Carr, Walter Kim, Hyung-Suk Yun, Sijung Walker, Peter Ahlers, Stephen LoPresti, Matthew Yarnell, Angela |
author_sort | Gill, Jessica |
collection | PubMed |
description | OBJECTIVE: To explore gene expression after moderate blast exposure (vs baseline) and proteomic changes after moderate- (vs low-) blast exposure. METHODS: Military personnel (N = 69) donated blood for quantification of protein level, and peak pressure exposures were detected by helmet sensors before and during a blast training program (10 days total). On day 7, some participants (n = 29) sustained a moderate blast (mean peak pressure = 7.9 psi) and were matched to participants with no/low-blast exposure during the training (n = 40). PAXgene tubes were collected from one training site at baseline and day 10; RNA-sequencing day 10 expression was compared with each participant's own baseline samples to identify genes and pathways differentially expressed in moderate blast-exposed participants. Changes in amyloid precursor protein (APP) from baseline to the day of blast and following 2 days were evaluated. Symptoms were assessed using a self-reported form. RESULTS: We identified 1,803 differentially expressed genes after moderate blast exposure; the most altered network was APP. Significantly reduced levels of peripheral APP were detected the day after the moderate blast exposure and the following day. Protein concentrations correlated with the magnitude of the moderate blast exposure on days 8 and 9. APP concentrations returned to baseline levels 3 days following the blast, likely due to increases in the genetic expression of APP. Onset of concentration problems and headaches occurred after moderate blast. CONCLUSIONS: Moderate blast exposure results in a signature biological profile that includes acute APP reductions, followed by genetic expression increases and normalization of APP levels; these changes likely influence neuronal recovery. |
format | Online Article Text |
id | pubmed-5618107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Wolters Kluwer |
record_format | MEDLINE/PubMed |
spelling | pubmed-56181072017-10-03 Moderate blast exposure alters gene expression and levels of amyloid precursor protein Gill, Jessica Cashion, Ann Osier, Nicole Arcurio, Lindsay Motamedi, Vida Dell, Kristine C. Carr, Walter Kim, Hyung-Suk Yun, Sijung Walker, Peter Ahlers, Stephen LoPresti, Matthew Yarnell, Angela Neurol Genet Article OBJECTIVE: To explore gene expression after moderate blast exposure (vs baseline) and proteomic changes after moderate- (vs low-) blast exposure. METHODS: Military personnel (N = 69) donated blood for quantification of protein level, and peak pressure exposures were detected by helmet sensors before and during a blast training program (10 days total). On day 7, some participants (n = 29) sustained a moderate blast (mean peak pressure = 7.9 psi) and were matched to participants with no/low-blast exposure during the training (n = 40). PAXgene tubes were collected from one training site at baseline and day 10; RNA-sequencing day 10 expression was compared with each participant's own baseline samples to identify genes and pathways differentially expressed in moderate blast-exposed participants. Changes in amyloid precursor protein (APP) from baseline to the day of blast and following 2 days were evaluated. Symptoms were assessed using a self-reported form. RESULTS: We identified 1,803 differentially expressed genes after moderate blast exposure; the most altered network was APP. Significantly reduced levels of peripheral APP were detected the day after the moderate blast exposure and the following day. Protein concentrations correlated with the magnitude of the moderate blast exposure on days 8 and 9. APP concentrations returned to baseline levels 3 days following the blast, likely due to increases in the genetic expression of APP. Onset of concentration problems and headaches occurred after moderate blast. CONCLUSIONS: Moderate blast exposure results in a signature biological profile that includes acute APP reductions, followed by genetic expression increases and normalization of APP levels; these changes likely influence neuronal recovery. Wolters Kluwer 2017-09-27 /pmc/articles/PMC5618107/ /pubmed/28975156 http://dx.doi.org/10.1212/NXG.0000000000000186 Text en Copyright © 2017 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. |
spellingShingle | Article Gill, Jessica Cashion, Ann Osier, Nicole Arcurio, Lindsay Motamedi, Vida Dell, Kristine C. Carr, Walter Kim, Hyung-Suk Yun, Sijung Walker, Peter Ahlers, Stephen LoPresti, Matthew Yarnell, Angela Moderate blast exposure alters gene expression and levels of amyloid precursor protein |
title | Moderate blast exposure alters gene expression and levels of amyloid precursor protein |
title_full | Moderate blast exposure alters gene expression and levels of amyloid precursor protein |
title_fullStr | Moderate blast exposure alters gene expression and levels of amyloid precursor protein |
title_full_unstemmed | Moderate blast exposure alters gene expression and levels of amyloid precursor protein |
title_short | Moderate blast exposure alters gene expression and levels of amyloid precursor protein |
title_sort | moderate blast exposure alters gene expression and levels of amyloid precursor protein |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618107/ https://www.ncbi.nlm.nih.gov/pubmed/28975156 http://dx.doi.org/10.1212/NXG.0000000000000186 |
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