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Sinapic Acid Affects Phenolic and Trichothecene Profiles of F. culmorum and F. graminearum Sensu Stricto

Plant-derived compounds for reducing the mycotoxin load in food and feed have become a rapidly developing research field of importance for plant breeding efforts and in the search for natural fungicides. In this study, toxigenic strains of Fusarium culmorum and F. graminearum sensu stricto were expo...

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Autores principales: Kulik, Tomasz, Stuper-Szablewska, Kinga, Bilska, Katarzyna, Buśko, Maciej, Ostrowska-Kołodziejczak, Anna, Załuski, Dariusz, Perkowski, Juliusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618197/
https://www.ncbi.nlm.nih.gov/pubmed/28846647
http://dx.doi.org/10.3390/toxins9090264
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author Kulik, Tomasz
Stuper-Szablewska, Kinga
Bilska, Katarzyna
Buśko, Maciej
Ostrowska-Kołodziejczak, Anna
Załuski, Dariusz
Perkowski, Juliusz
author_facet Kulik, Tomasz
Stuper-Szablewska, Kinga
Bilska, Katarzyna
Buśko, Maciej
Ostrowska-Kołodziejczak, Anna
Załuski, Dariusz
Perkowski, Juliusz
author_sort Kulik, Tomasz
collection PubMed
description Plant-derived compounds for reducing the mycotoxin load in food and feed have become a rapidly developing research field of importance for plant breeding efforts and in the search for natural fungicides. In this study, toxigenic strains of Fusarium culmorum and F. graminearum sensu stricto were exposed to sinapic acid on solid YES media at levels close to those reported in wheat bran. Fusaria produced phenolic acids, whose accumulation was decreased by exogenous sinapic acid. Strains exposed to the lowest doses of sinapic acid showed more efficient reduction of phenolic acid production than fungi kept at higher concentrations of this compound. Fungi reduced exogenous sinapic acid, leading to the formation of syringic aldehyde. Treatment with sinapic acid led to a dramatic accumulation of its parent compound ferulic acid, presumably due to inhibition of the further conversion of this phenolic compound. Exogenous sinapic acid decreased the production of trichothecenes by fungi. Higher doses of sinapic acid resulted in more efficient reduction of mycotoxin accumulation in the media. Gene expression studies of Tri genes responsible for trichothecene biosynthesis (Tri4, Tri5 and Tri10) proved that the inhibition of mycotoxin production by sinapic acid occurred at the transcriptional level. Fusaria respond to sinapic acid by stimulation of ergosterol biosynthesis.
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spelling pubmed-56181972017-09-29 Sinapic Acid Affects Phenolic and Trichothecene Profiles of F. culmorum and F. graminearum Sensu Stricto Kulik, Tomasz Stuper-Szablewska, Kinga Bilska, Katarzyna Buśko, Maciej Ostrowska-Kołodziejczak, Anna Załuski, Dariusz Perkowski, Juliusz Toxins (Basel) Article Plant-derived compounds for reducing the mycotoxin load in food and feed have become a rapidly developing research field of importance for plant breeding efforts and in the search for natural fungicides. In this study, toxigenic strains of Fusarium culmorum and F. graminearum sensu stricto were exposed to sinapic acid on solid YES media at levels close to those reported in wheat bran. Fusaria produced phenolic acids, whose accumulation was decreased by exogenous sinapic acid. Strains exposed to the lowest doses of sinapic acid showed more efficient reduction of phenolic acid production than fungi kept at higher concentrations of this compound. Fungi reduced exogenous sinapic acid, leading to the formation of syringic aldehyde. Treatment with sinapic acid led to a dramatic accumulation of its parent compound ferulic acid, presumably due to inhibition of the further conversion of this phenolic compound. Exogenous sinapic acid decreased the production of trichothecenes by fungi. Higher doses of sinapic acid resulted in more efficient reduction of mycotoxin accumulation in the media. Gene expression studies of Tri genes responsible for trichothecene biosynthesis (Tri4, Tri5 and Tri10) proved that the inhibition of mycotoxin production by sinapic acid occurred at the transcriptional level. Fusaria respond to sinapic acid by stimulation of ergosterol biosynthesis. MDPI 2017-08-28 /pmc/articles/PMC5618197/ /pubmed/28846647 http://dx.doi.org/10.3390/toxins9090264 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kulik, Tomasz
Stuper-Szablewska, Kinga
Bilska, Katarzyna
Buśko, Maciej
Ostrowska-Kołodziejczak, Anna
Załuski, Dariusz
Perkowski, Juliusz
Sinapic Acid Affects Phenolic and Trichothecene Profiles of F. culmorum and F. graminearum Sensu Stricto
title Sinapic Acid Affects Phenolic and Trichothecene Profiles of F. culmorum and F. graminearum Sensu Stricto
title_full Sinapic Acid Affects Phenolic and Trichothecene Profiles of F. culmorum and F. graminearum Sensu Stricto
title_fullStr Sinapic Acid Affects Phenolic and Trichothecene Profiles of F. culmorum and F. graminearum Sensu Stricto
title_full_unstemmed Sinapic Acid Affects Phenolic and Trichothecene Profiles of F. culmorum and F. graminearum Sensu Stricto
title_short Sinapic Acid Affects Phenolic and Trichothecene Profiles of F. culmorum and F. graminearum Sensu Stricto
title_sort sinapic acid affects phenolic and trichothecene profiles of f. culmorum and f. graminearum sensu stricto
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618197/
https://www.ncbi.nlm.nih.gov/pubmed/28846647
http://dx.doi.org/10.3390/toxins9090264
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