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Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units

Staphylococcus aureus is a major cause of infections. Toxic shock syndrome toxin (TSST-1) and Panton–Valentine leukocidin (PVL) are associated with severe clinical syndromes. S. aureus colonizing isolates recovered from healthcare workers and patients in the intensive care unit (ICU) of a university...

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Autores principales: Papadimitriou-Olivgeris, Matthaios, Drougka, Eleanna, Fligou, Fotini, Dodou, Vasiliki, Kolonitsiou, Fevronia, Filos, Kriton S., Anastassiou, Evangelos D., Petinaki, Efthimia, Marangos, Markos, Spiliopoulou, Iris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618203/
https://www.ncbi.nlm.nih.gov/pubmed/28869541
http://dx.doi.org/10.3390/toxins9090270
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author Papadimitriou-Olivgeris, Matthaios
Drougka, Eleanna
Fligou, Fotini
Dodou, Vasiliki
Kolonitsiou, Fevronia
Filos, Kriton S.
Anastassiou, Evangelos D.
Petinaki, Efthimia
Marangos, Markos
Spiliopoulou, Iris
author_facet Papadimitriou-Olivgeris, Matthaios
Drougka, Eleanna
Fligou, Fotini
Dodou, Vasiliki
Kolonitsiou, Fevronia
Filos, Kriton S.
Anastassiou, Evangelos D.
Petinaki, Efthimia
Marangos, Markos
Spiliopoulou, Iris
author_sort Papadimitriou-Olivgeris, Matthaios
collection PubMed
description Staphylococcus aureus is a major cause of infections. Toxic shock syndrome toxin (TSST-1) and Panton–Valentine leukocidin (PVL) are associated with severe clinical syndromes. S. aureus colonizing isolates recovered from healthcare workers and patients in the intensive care unit (ICU) of a university hospital comprising Group A were compared with those from adult non-ICU carriers (Group B). mecA, lukS/lukF-PV (Panton–Valentine leukocidin, PVL), and tst (toxic shock syndrome toxin) gene carriage was detected by PCR. Clones were identified in all methicillin-resistant S. aureus (MRSA) and toxin-positive methicillin-susceptible strains (MSSA) by pulsed-field gel electrophoresis (PFGE), agr groups, and multi locus sequencing typing (MLST). Group A included 90 S. aureus isolates, whereas Group B 53. PVL was more frequently found among MRSA vs. MSSA (p < 0.001) and in strains of Group B as compared to Group A (p < 0.001), consistent with the spread of ST80-IV. Higher incidence of tst gene carriage was identified among MSSA vs. MRSA (P 0.005) belonging mainly to ST30, and Group A vs. Group B (P 0.002). The wide dissemination of ST80-IV mainly in the community is responsible for a high percentage of PVL-positive MRSA, while silent spread of tst-positive S. aureus clones among ICU patients and personnel poses a threat of hospital transmission and possible severe infections.
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spelling pubmed-56182032017-09-29 Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units Papadimitriou-Olivgeris, Matthaios Drougka, Eleanna Fligou, Fotini Dodou, Vasiliki Kolonitsiou, Fevronia Filos, Kriton S. Anastassiou, Evangelos D. Petinaki, Efthimia Marangos, Markos Spiliopoulou, Iris Toxins (Basel) Article Staphylococcus aureus is a major cause of infections. Toxic shock syndrome toxin (TSST-1) and Panton–Valentine leukocidin (PVL) are associated with severe clinical syndromes. S. aureus colonizing isolates recovered from healthcare workers and patients in the intensive care unit (ICU) of a university hospital comprising Group A were compared with those from adult non-ICU carriers (Group B). mecA, lukS/lukF-PV (Panton–Valentine leukocidin, PVL), and tst (toxic shock syndrome toxin) gene carriage was detected by PCR. Clones were identified in all methicillin-resistant S. aureus (MRSA) and toxin-positive methicillin-susceptible strains (MSSA) by pulsed-field gel electrophoresis (PFGE), agr groups, and multi locus sequencing typing (MLST). Group A included 90 S. aureus isolates, whereas Group B 53. PVL was more frequently found among MRSA vs. MSSA (p < 0.001) and in strains of Group B as compared to Group A (p < 0.001), consistent with the spread of ST80-IV. Higher incidence of tst gene carriage was identified among MSSA vs. MRSA (P 0.005) belonging mainly to ST30, and Group A vs. Group B (P 0.002). The wide dissemination of ST80-IV mainly in the community is responsible for a high percentage of PVL-positive MRSA, while silent spread of tst-positive S. aureus clones among ICU patients and personnel poses a threat of hospital transmission and possible severe infections. MDPI 2017-09-04 /pmc/articles/PMC5618203/ /pubmed/28869541 http://dx.doi.org/10.3390/toxins9090270 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Papadimitriou-Olivgeris, Matthaios
Drougka, Eleanna
Fligou, Fotini
Dodou, Vasiliki
Kolonitsiou, Fevronia
Filos, Kriton S.
Anastassiou, Evangelos D.
Petinaki, Efthimia
Marangos, Markos
Spiliopoulou, Iris
Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units
title Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units
title_full Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units
title_fullStr Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units
title_full_unstemmed Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units
title_short Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units
title_sort spread of tst–positive staphylococcus aureus strains belonging to st30 clone among patients and healthcare workers in two intensive care units
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618203/
https://www.ncbi.nlm.nih.gov/pubmed/28869541
http://dx.doi.org/10.3390/toxins9090270
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