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Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications

Polyethylene Glycol Diacrylate (PEGDA) tissue scaffolds having a thickness higher than 1 mm and without the presence of nutrient conduit networks were shown to have limited applications in tissue engineering due to the inability of cells to adhere and migrate within the scaffold. The PEGDA scaffold...

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Autores principales: Kotturi, Hari, Abuabed, Alaeddin, Zafar, Haris, Sawyer, Elaine, Pallipparambil, Bipin, Jamadagni, Harsha, Khandaker, Morshed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618290/
https://www.ncbi.nlm.nih.gov/pubmed/28872610
http://dx.doi.org/10.3390/jfb8030039
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author Kotturi, Hari
Abuabed, Alaeddin
Zafar, Haris
Sawyer, Elaine
Pallipparambil, Bipin
Jamadagni, Harsha
Khandaker, Morshed
author_facet Kotturi, Hari
Abuabed, Alaeddin
Zafar, Haris
Sawyer, Elaine
Pallipparambil, Bipin
Jamadagni, Harsha
Khandaker, Morshed
author_sort Kotturi, Hari
collection PubMed
description Polyethylene Glycol Diacrylate (PEGDA) tissue scaffolds having a thickness higher than 1 mm and without the presence of nutrient conduit networks were shown to have limited applications in tissue engineering due to the inability of cells to adhere and migrate within the scaffold. The PEGDA scaffold has been coated with polycaprolactone (PCL) electrospun nanofiber (ENF) membrane on both sides to overcome these limitations, thereby creating a functional PEGDA-PCL scaffold. This study examined the physical, mechanical, and biological properties of the PEGDA and PEGDA-PCL scaffolds to determine the effect of PCL coating on PEGDA. The physical characterization of PEGDA-PCL samples demonstrated the effectiveness of combining PCL with a PEGDA scaffold to expand its applications in tissue engineering. This study also found a significant improvement of elasticity of PEGDA due to the addition of PCL layers. This study shows that PEGDA-PCL scaffolds absorb nutrients with time and can provide an ideal environment for the survival of cells. Furthermore, cell viability tests indicate that the cell adhered, proliferated, and migrated in the PEGDA-PCL scaffold. Therefore, PCL ENF coating has a positive influence on PEGDA scaffold.
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spelling pubmed-56182902017-09-29 Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications Kotturi, Hari Abuabed, Alaeddin Zafar, Haris Sawyer, Elaine Pallipparambil, Bipin Jamadagni, Harsha Khandaker, Morshed J Funct Biomater Article Polyethylene Glycol Diacrylate (PEGDA) tissue scaffolds having a thickness higher than 1 mm and without the presence of nutrient conduit networks were shown to have limited applications in tissue engineering due to the inability of cells to adhere and migrate within the scaffold. The PEGDA scaffold has been coated with polycaprolactone (PCL) electrospun nanofiber (ENF) membrane on both sides to overcome these limitations, thereby creating a functional PEGDA-PCL scaffold. This study examined the physical, mechanical, and biological properties of the PEGDA and PEGDA-PCL scaffolds to determine the effect of PCL coating on PEGDA. The physical characterization of PEGDA-PCL samples demonstrated the effectiveness of combining PCL with a PEGDA scaffold to expand its applications in tissue engineering. This study also found a significant improvement of elasticity of PEGDA due to the addition of PCL layers. This study shows that PEGDA-PCL scaffolds absorb nutrients with time and can provide an ideal environment for the survival of cells. Furthermore, cell viability tests indicate that the cell adhered, proliferated, and migrated in the PEGDA-PCL scaffold. Therefore, PCL ENF coating has a positive influence on PEGDA scaffold. MDPI 2017-09-05 /pmc/articles/PMC5618290/ /pubmed/28872610 http://dx.doi.org/10.3390/jfb8030039 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kotturi, Hari
Abuabed, Alaeddin
Zafar, Haris
Sawyer, Elaine
Pallipparambil, Bipin
Jamadagni, Harsha
Khandaker, Morshed
Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications
title Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications
title_full Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications
title_fullStr Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications
title_full_unstemmed Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications
title_short Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications
title_sort evaluation of polyethylene glycol diacrylate-polycaprolactone scaffolds for tissue engineering applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618290/
https://www.ncbi.nlm.nih.gov/pubmed/28872610
http://dx.doi.org/10.3390/jfb8030039
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