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Engineered Aptamers to Probe Molecular Interactions on the Cell Surface

Significant progress has been made in understanding the nature of molecular interactions on the cell membrane. To decipher such interactions, molecular scaffolds can be engineered as a tool to modulate these events as they occur on the cell membrane. To guarantee reliability, scaffolds that function...

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Autores principales: Batool, Sana, Bhandari, Sanam, George, Shanell, Okeoma, Precious, Van, Nabeela, Zümrüt, Hazan E., Mallikaratchy, Prabodhika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618312/
https://www.ncbi.nlm.nih.gov/pubmed/28850067
http://dx.doi.org/10.3390/biomedicines5030054
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author Batool, Sana
Bhandari, Sanam
George, Shanell
Okeoma, Precious
Van, Nabeela
Zümrüt, Hazan E.
Mallikaratchy, Prabodhika
author_facet Batool, Sana
Bhandari, Sanam
George, Shanell
Okeoma, Precious
Van, Nabeela
Zümrüt, Hazan E.
Mallikaratchy, Prabodhika
author_sort Batool, Sana
collection PubMed
description Significant progress has been made in understanding the nature of molecular interactions on the cell membrane. To decipher such interactions, molecular scaffolds can be engineered as a tool to modulate these events as they occur on the cell membrane. To guarantee reliability, scaffolds that function as modulators of cell membrane events must be coupled to a targeting moiety with superior chemical versatility. In this regard, nucleic acid aptamers are a suitable class of targeting moieties. Aptamers are inherently chemical in nature, allowing extensive site-specific chemical modification to engineer sensing molecules. Aptamers can be easily selected using a simple laboratory-based in vitro evolution method enabling the design and development of aptamer-based functional molecular scaffolds against wide range of cell surface molecules. This article reviews the application of aptamers as monitors and modulators of molecular interactions on the mammalian cell surface with the aim of increasing our understanding of cell-surface receptor response to external stimuli. The information gained from these types of studies could eventually prove useful in engineering improved medical diagnostics and therapeutics.
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spelling pubmed-56183122017-09-29 Engineered Aptamers to Probe Molecular Interactions on the Cell Surface Batool, Sana Bhandari, Sanam George, Shanell Okeoma, Precious Van, Nabeela Zümrüt, Hazan E. Mallikaratchy, Prabodhika Biomedicines Review Significant progress has been made in understanding the nature of molecular interactions on the cell membrane. To decipher such interactions, molecular scaffolds can be engineered as a tool to modulate these events as they occur on the cell membrane. To guarantee reliability, scaffolds that function as modulators of cell membrane events must be coupled to a targeting moiety with superior chemical versatility. In this regard, nucleic acid aptamers are a suitable class of targeting moieties. Aptamers are inherently chemical in nature, allowing extensive site-specific chemical modification to engineer sensing molecules. Aptamers can be easily selected using a simple laboratory-based in vitro evolution method enabling the design and development of aptamer-based functional molecular scaffolds against wide range of cell surface molecules. This article reviews the application of aptamers as monitors and modulators of molecular interactions on the mammalian cell surface with the aim of increasing our understanding of cell-surface receptor response to external stimuli. The information gained from these types of studies could eventually prove useful in engineering improved medical diagnostics and therapeutics. MDPI 2017-08-29 /pmc/articles/PMC5618312/ /pubmed/28850067 http://dx.doi.org/10.3390/biomedicines5030054 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Batool, Sana
Bhandari, Sanam
George, Shanell
Okeoma, Precious
Van, Nabeela
Zümrüt, Hazan E.
Mallikaratchy, Prabodhika
Engineered Aptamers to Probe Molecular Interactions on the Cell Surface
title Engineered Aptamers to Probe Molecular Interactions on the Cell Surface
title_full Engineered Aptamers to Probe Molecular Interactions on the Cell Surface
title_fullStr Engineered Aptamers to Probe Molecular Interactions on the Cell Surface
title_full_unstemmed Engineered Aptamers to Probe Molecular Interactions on the Cell Surface
title_short Engineered Aptamers to Probe Molecular Interactions on the Cell Surface
title_sort engineered aptamers to probe molecular interactions on the cell surface
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618312/
https://www.ncbi.nlm.nih.gov/pubmed/28850067
http://dx.doi.org/10.3390/biomedicines5030054
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