Cargando…

Integrated Metabolomics Assessment of Human Dried Blood Spots and Urine Strips

(1) Background: Interest in the application of metabolomics toward clinical diagnostics development and population health monitoring has grown significantly in recent years. In spite of several advances in analytical and computational tools, obtaining a sufficient number of samples from patients rem...

Descripción completa

Detalles Bibliográficos
Autores principales: Drolet, Jeremy, Tolstikov, Vladimir, Williams, Brian A., Greenwood, Bennett P., Hill, Collin, Vishnudas, Vivek K., Sarangarajan, Rangaprasad, Narain, Niven R., Kiebish, Michael A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618320/
https://www.ncbi.nlm.nih.gov/pubmed/28714878
http://dx.doi.org/10.3390/metabo7030035
_version_ 1783267159868178432
author Drolet, Jeremy
Tolstikov, Vladimir
Williams, Brian A.
Greenwood, Bennett P.
Hill, Collin
Vishnudas, Vivek K.
Sarangarajan, Rangaprasad
Narain, Niven R.
Kiebish, Michael A.
author_facet Drolet, Jeremy
Tolstikov, Vladimir
Williams, Brian A.
Greenwood, Bennett P.
Hill, Collin
Vishnudas, Vivek K.
Sarangarajan, Rangaprasad
Narain, Niven R.
Kiebish, Michael A.
author_sort Drolet, Jeremy
collection PubMed
description (1) Background: Interest in the application of metabolomics toward clinical diagnostics development and population health monitoring has grown significantly in recent years. In spite of several advances in analytical and computational tools, obtaining a sufficient number of samples from patients remains an obstacle. The dried blood spot (DBS) and dried urine strip (DUS) methodologies are a minimally invasive sample collection method allowing for the relative simplicity of sample collection and minimal cost. (2) Methods: In the current report, we compared results of targeted metabolomics analyses of four types of human blood sample collection methods (with and without DBS) and two types of urine sample collection (DUS and urine) across several parameters including the metabolite coverage of each matrix and the sample stability for DBS/DUS using commercially available Whatman 903TM paper. The DBS/DUS metabolomics protocols were further applied to examine the temporal metabolite level fluctuations within hours and days of sample collection. (3) Results: Several hundred polar metabolites were monitored using DBS/DUS. Temporal analysis of the polar metabolites at various times of the day and across days identified several species that fluctuate as a function of day and time. In addition, a subset of metabolites were identified to be significantly altered across hours within a day and within successive days of the week. (4) Conclusion: A comprehensive DBS/DUS metabolomics protocol was developed for human blood and urine analyses. The described methodology demonstrates the potential for enabling patients to contribute to the expanding bioanalytical demands of precision medicine and population health studies.
format Online
Article
Text
id pubmed-5618320
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-56183202017-09-29 Integrated Metabolomics Assessment of Human Dried Blood Spots and Urine Strips Drolet, Jeremy Tolstikov, Vladimir Williams, Brian A. Greenwood, Bennett P. Hill, Collin Vishnudas, Vivek K. Sarangarajan, Rangaprasad Narain, Niven R. Kiebish, Michael A. Metabolites Article (1) Background: Interest in the application of metabolomics toward clinical diagnostics development and population health monitoring has grown significantly in recent years. In spite of several advances in analytical and computational tools, obtaining a sufficient number of samples from patients remains an obstacle. The dried blood spot (DBS) and dried urine strip (DUS) methodologies are a minimally invasive sample collection method allowing for the relative simplicity of sample collection and minimal cost. (2) Methods: In the current report, we compared results of targeted metabolomics analyses of four types of human blood sample collection methods (with and without DBS) and two types of urine sample collection (DUS and urine) across several parameters including the metabolite coverage of each matrix and the sample stability for DBS/DUS using commercially available Whatman 903TM paper. The DBS/DUS metabolomics protocols were further applied to examine the temporal metabolite level fluctuations within hours and days of sample collection. (3) Results: Several hundred polar metabolites were monitored using DBS/DUS. Temporal analysis of the polar metabolites at various times of the day and across days identified several species that fluctuate as a function of day and time. In addition, a subset of metabolites were identified to be significantly altered across hours within a day and within successive days of the week. (4) Conclusion: A comprehensive DBS/DUS metabolomics protocol was developed for human blood and urine analyses. The described methodology demonstrates the potential for enabling patients to contribute to the expanding bioanalytical demands of precision medicine and population health studies. MDPI 2017-07-15 /pmc/articles/PMC5618320/ /pubmed/28714878 http://dx.doi.org/10.3390/metabo7030035 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Drolet, Jeremy
Tolstikov, Vladimir
Williams, Brian A.
Greenwood, Bennett P.
Hill, Collin
Vishnudas, Vivek K.
Sarangarajan, Rangaprasad
Narain, Niven R.
Kiebish, Michael A.
Integrated Metabolomics Assessment of Human Dried Blood Spots and Urine Strips
title Integrated Metabolomics Assessment of Human Dried Blood Spots and Urine Strips
title_full Integrated Metabolomics Assessment of Human Dried Blood Spots and Urine Strips
title_fullStr Integrated Metabolomics Assessment of Human Dried Blood Spots and Urine Strips
title_full_unstemmed Integrated Metabolomics Assessment of Human Dried Blood Spots and Urine Strips
title_short Integrated Metabolomics Assessment of Human Dried Blood Spots and Urine Strips
title_sort integrated metabolomics assessment of human dried blood spots and urine strips
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618320/
https://www.ncbi.nlm.nih.gov/pubmed/28714878
http://dx.doi.org/10.3390/metabo7030035
work_keys_str_mv AT droletjeremy integratedmetabolomicsassessmentofhumandriedbloodspotsandurinestrips
AT tolstikovvladimir integratedmetabolomicsassessmentofhumandriedbloodspotsandurinestrips
AT williamsbriana integratedmetabolomicsassessmentofhumandriedbloodspotsandurinestrips
AT greenwoodbennettp integratedmetabolomicsassessmentofhumandriedbloodspotsandurinestrips
AT hillcollin integratedmetabolomicsassessmentofhumandriedbloodspotsandurinestrips
AT vishnudasvivekk integratedmetabolomicsassessmentofhumandriedbloodspotsandurinestrips
AT sarangarajanrangaprasad integratedmetabolomicsassessmentofhumandriedbloodspotsandurinestrips
AT narainnivenr integratedmetabolomicsassessmentofhumandriedbloodspotsandurinestrips
AT kiebishmichaela integratedmetabolomicsassessmentofhumandriedbloodspotsandurinestrips