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Construction of a Fluorescent H(2)O(2) Biosensor with Chitosan 6-OH Immobilized β-Cyclodextrin Derivatives
In the present work, a fluorescent H(2)O(2) biosensor was constructed by encapsulating fluorescent probe Rhodamine B (RhmB) in the hydrophobic cavity of the cyclodextrin (β-CD) and immobilizing catalase (CAT) on the 2-NH(2) of chitosan (CTS) in a chitosan 6-OH immobilized β-cyclodextrin derivative (...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618423/ https://www.ncbi.nlm.nih.gov/pubmed/28869533 http://dx.doi.org/10.3390/md15090284 |
Sumario: | In the present work, a fluorescent H(2)O(2) biosensor was constructed by encapsulating fluorescent probe Rhodamine B (RhmB) in the hydrophobic cavity of the cyclodextrin (β-CD) and immobilizing catalase (CAT) on the 2-NH(2) of chitosan (CTS) in a chitosan 6-OH immobilized β-cyclodextrin derivative (CTS-6-CD). The inclusion complex of CTS-6-CD to RhmB (CTS-6-CD-RhmB) was prepared by a solution method. Its structure and inclusion efficiency were determined by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and fluorescence spectroscopy (FL). CAT was immobilized on CTS-6-CD-RhmB to eventually form the functional membrane, CTS-6-CD-RhmB-CAT, via glutaraldehyde crosslinking, which was further characterized by FTIR and FL, and used as a H(2)O(2) biosensor. The functional membrane was used to simultaneously oxidize and detect H(2)O(2). The detection condition was optimized as pH 8, a reaction temperature of 25 °C, and an immobilized enzyme concentration of 2 × 10(−4) mol/L. The fluorescence response of the biosensor exhibited a good linear relationship with the concentration of H(2)O(2) in the range of 20 mΜ–300 μM and the detection limit of 10(−8) mol/L. |
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