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Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production

The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the g...

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Autores principales: Ben Amor, Faten, Elleuch, Fatma, Ben Hlima, Hajer, Garnier, Matthieu, Saint-Jean, Bruno, Barkallah, Mohamed, Pichon, Chantal, Abdelkafi, Slim, Fendri, Imen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618432/
https://www.ncbi.nlm.nih.gov/pubmed/28930152
http://dx.doi.org/10.3390/md15090293
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author Ben Amor, Faten
Elleuch, Fatma
Ben Hlima, Hajer
Garnier, Matthieu
Saint-Jean, Bruno
Barkallah, Mohamed
Pichon, Chantal
Abdelkafi, Slim
Fendri, Imen
author_facet Ben Amor, Faten
Elleuch, Fatma
Ben Hlima, Hajer
Garnier, Matthieu
Saint-Jean, Bruno
Barkallah, Mohamed
Pichon, Chantal
Abdelkafi, Slim
Fendri, Imen
author_sort Ben Amor, Faten
collection PubMed
description The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m(−2)·s(−1), and nitrogen: 60 mg·L(−1). Proteomic profiling, using two-dimensional gel electrophoresis, was performed from standard and optimized cultures. We detected 127 protein spots which were significantly differentially expressed between standard and optimized cultures. Among them 16 protein spots were identified with mass spectrometry and grouped into different functional categories using KEGG (Kyoto Encyclopedia of Genes and Genomes) such as photosynthetic Calvin cycle, regulation/defense, energy metabolism, glycolysis, and cellular processes. The current study could be of great interest in providing information on the effect of stressful conditions in microalgae carotenoid production.
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spelling pubmed-56184322017-09-30 Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production Ben Amor, Faten Elleuch, Fatma Ben Hlima, Hajer Garnier, Matthieu Saint-Jean, Bruno Barkallah, Mohamed Pichon, Chantal Abdelkafi, Slim Fendri, Imen Mar Drugs Article The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m(−2)·s(−1), and nitrogen: 60 mg·L(−1). Proteomic profiling, using two-dimensional gel electrophoresis, was performed from standard and optimized cultures. We detected 127 protein spots which were significantly differentially expressed between standard and optimized cultures. Among them 16 protein spots were identified with mass spectrometry and grouped into different functional categories using KEGG (Kyoto Encyclopedia of Genes and Genomes) such as photosynthetic Calvin cycle, regulation/defense, energy metabolism, glycolysis, and cellular processes. The current study could be of great interest in providing information on the effect of stressful conditions in microalgae carotenoid production. MDPI 2017-09-20 /pmc/articles/PMC5618432/ /pubmed/28930152 http://dx.doi.org/10.3390/md15090293 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ben Amor, Faten
Elleuch, Fatma
Ben Hlima, Hajer
Garnier, Matthieu
Saint-Jean, Bruno
Barkallah, Mohamed
Pichon, Chantal
Abdelkafi, Slim
Fendri, Imen
Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production
title Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production
title_full Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production
title_fullStr Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production
title_full_unstemmed Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production
title_short Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production
title_sort proteomic analysis of the chlorophyta dunaliella new strain al-1 revealed global changes of metabolism during high carotenoid production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618432/
https://www.ncbi.nlm.nih.gov/pubmed/28930152
http://dx.doi.org/10.3390/md15090293
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