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Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition
The hard-shelled mussel (Mytilus coruscus) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to Mytilus coruscus are still unclear. An α-d-Glucan, called MP-A, was isolated from Myt...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618433/ https://www.ncbi.nlm.nih.gov/pubmed/28930149 http://dx.doi.org/10.3390/md15090294 |
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author | Liu, Fuyan Zhang, Xiaofeng Li, Yuqiu Chen, Qixin Liu, Fei Zhu, Xiqiang Mei, Li Song, Xinlei Liu, Xia Song, Zhigang Zhang, Jinhua Zhang, Wen Ling, Peixue Wang, Fengshan |
author_facet | Liu, Fuyan Zhang, Xiaofeng Li, Yuqiu Chen, Qixin Liu, Fei Zhu, Xiqiang Mei, Li Song, Xinlei Liu, Xia Song, Zhigang Zhang, Jinhua Zhang, Wen Ling, Peixue Wang, Fengshan |
author_sort | Liu, Fuyan |
collection | PubMed |
description | The hard-shelled mussel (Mytilus coruscus) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to Mytilus coruscus are still unclear. An α-d-Glucan, called MP-A, was isolated from Mytilus coruscus, and observed to exert anti-inflammatory activity in THP-1 human macrophage cells. Specifically, we showed that MP-A treatment inhibited the production of inflammatory markers, including TNF-α, NO, and PGE2, inducible NOS (iNOS), and cyclooxygenase-2 (COX-2), in LPS-activated THP-1 cells. It was also shown to enhance phagocytosis in the analyzed cells, but to severely inhibit the phosphorylation of mitogen-activated protein kinases (MAPKs) and the nuclear translocation of NF-κB P65. Finally, MP-A was found to exhibit a high binding affinity for the cell surface receptor TLR4, but a low affinity for TLR2 and dectin-1, via surface plasmon resonance (SPR) analysis. The study indicates that MP-A suppresses LPS-induced TNF-α, NO and PEG2 production via TLR4/NF-κB/MAPK pathway inhibition, and suggests that MP-A may be a promising therapeutic candidate for diseases associated with TNF-α, NO, and/or PEG2 overproduction. |
format | Online Article Text |
id | pubmed-5618433 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56184332017-09-30 Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition Liu, Fuyan Zhang, Xiaofeng Li, Yuqiu Chen, Qixin Liu, Fei Zhu, Xiqiang Mei, Li Song, Xinlei Liu, Xia Song, Zhigang Zhang, Jinhua Zhang, Wen Ling, Peixue Wang, Fengshan Mar Drugs Article The hard-shelled mussel (Mytilus coruscus) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to Mytilus coruscus are still unclear. An α-d-Glucan, called MP-A, was isolated from Mytilus coruscus, and observed to exert anti-inflammatory activity in THP-1 human macrophage cells. Specifically, we showed that MP-A treatment inhibited the production of inflammatory markers, including TNF-α, NO, and PGE2, inducible NOS (iNOS), and cyclooxygenase-2 (COX-2), in LPS-activated THP-1 cells. It was also shown to enhance phagocytosis in the analyzed cells, but to severely inhibit the phosphorylation of mitogen-activated protein kinases (MAPKs) and the nuclear translocation of NF-κB P65. Finally, MP-A was found to exhibit a high binding affinity for the cell surface receptor TLR4, but a low affinity for TLR2 and dectin-1, via surface plasmon resonance (SPR) analysis. The study indicates that MP-A suppresses LPS-induced TNF-α, NO and PEG2 production via TLR4/NF-κB/MAPK pathway inhibition, and suggests that MP-A may be a promising therapeutic candidate for diseases associated with TNF-α, NO, and/or PEG2 overproduction. MDPI 2017-09-20 /pmc/articles/PMC5618433/ /pubmed/28930149 http://dx.doi.org/10.3390/md15090294 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Fuyan Zhang, Xiaofeng Li, Yuqiu Chen, Qixin Liu, Fei Zhu, Xiqiang Mei, Li Song, Xinlei Liu, Xia Song, Zhigang Zhang, Jinhua Zhang, Wen Ling, Peixue Wang, Fengshan Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition |
title | Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition |
title_full | Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition |
title_fullStr | Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition |
title_full_unstemmed | Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition |
title_short | Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition |
title_sort | anti-inflammatory effects of a mytilus coruscus α-d-glucan (mp-a) in activated macrophage cells via tlr4/nf-κb/mapk pathway inhibition |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618433/ https://www.ncbi.nlm.nih.gov/pubmed/28930149 http://dx.doi.org/10.3390/md15090294 |
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