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Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition

The hard-shelled mussel (Mytilus coruscus) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to Mytilus coruscus are still unclear. An α-d-Glucan, called MP-A, was isolated from Myt...

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Autores principales: Liu, Fuyan, Zhang, Xiaofeng, Li, Yuqiu, Chen, Qixin, Liu, Fei, Zhu, Xiqiang, Mei, Li, Song, Xinlei, Liu, Xia, Song, Zhigang, Zhang, Jinhua, Zhang, Wen, Ling, Peixue, Wang, Fengshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618433/
https://www.ncbi.nlm.nih.gov/pubmed/28930149
http://dx.doi.org/10.3390/md15090294
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author Liu, Fuyan
Zhang, Xiaofeng
Li, Yuqiu
Chen, Qixin
Liu, Fei
Zhu, Xiqiang
Mei, Li
Song, Xinlei
Liu, Xia
Song, Zhigang
Zhang, Jinhua
Zhang, Wen
Ling, Peixue
Wang, Fengshan
author_facet Liu, Fuyan
Zhang, Xiaofeng
Li, Yuqiu
Chen, Qixin
Liu, Fei
Zhu, Xiqiang
Mei, Li
Song, Xinlei
Liu, Xia
Song, Zhigang
Zhang, Jinhua
Zhang, Wen
Ling, Peixue
Wang, Fengshan
author_sort Liu, Fuyan
collection PubMed
description The hard-shelled mussel (Mytilus coruscus) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to Mytilus coruscus are still unclear. An α-d-Glucan, called MP-A, was isolated from Mytilus coruscus, and observed to exert anti-inflammatory activity in THP-1 human macrophage cells. Specifically, we showed that MP-A treatment inhibited the production of inflammatory markers, including TNF-α, NO, and PGE2, inducible NOS (iNOS), and cyclooxygenase-2 (COX-2), in LPS-activated THP-1 cells. It was also shown to enhance phagocytosis in the analyzed cells, but to severely inhibit the phosphorylation of mitogen-activated protein kinases (MAPKs) and the nuclear translocation of NF-κB P65. Finally, MP-A was found to exhibit a high binding affinity for the cell surface receptor TLR4, but a low affinity for TLR2 and dectin-1, via surface plasmon resonance (SPR) analysis. The study indicates that MP-A suppresses LPS-induced TNF-α, NO and PEG2 production via TLR4/NF-κB/MAPK pathway inhibition, and suggests that MP-A may be a promising therapeutic candidate for diseases associated with TNF-α, NO, and/or PEG2 overproduction.
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spelling pubmed-56184332017-09-30 Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition Liu, Fuyan Zhang, Xiaofeng Li, Yuqiu Chen, Qixin Liu, Fei Zhu, Xiqiang Mei, Li Song, Xinlei Liu, Xia Song, Zhigang Zhang, Jinhua Zhang, Wen Ling, Peixue Wang, Fengshan Mar Drugs Article The hard-shelled mussel (Mytilus coruscus) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to Mytilus coruscus are still unclear. An α-d-Glucan, called MP-A, was isolated from Mytilus coruscus, and observed to exert anti-inflammatory activity in THP-1 human macrophage cells. Specifically, we showed that MP-A treatment inhibited the production of inflammatory markers, including TNF-α, NO, and PGE2, inducible NOS (iNOS), and cyclooxygenase-2 (COX-2), in LPS-activated THP-1 cells. It was also shown to enhance phagocytosis in the analyzed cells, but to severely inhibit the phosphorylation of mitogen-activated protein kinases (MAPKs) and the nuclear translocation of NF-κB P65. Finally, MP-A was found to exhibit a high binding affinity for the cell surface receptor TLR4, but a low affinity for TLR2 and dectin-1, via surface plasmon resonance (SPR) analysis. The study indicates that MP-A suppresses LPS-induced TNF-α, NO and PEG2 production via TLR4/NF-κB/MAPK pathway inhibition, and suggests that MP-A may be a promising therapeutic candidate for diseases associated with TNF-α, NO, and/or PEG2 overproduction. MDPI 2017-09-20 /pmc/articles/PMC5618433/ /pubmed/28930149 http://dx.doi.org/10.3390/md15090294 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Fuyan
Zhang, Xiaofeng
Li, Yuqiu
Chen, Qixin
Liu, Fei
Zhu, Xiqiang
Mei, Li
Song, Xinlei
Liu, Xia
Song, Zhigang
Zhang, Jinhua
Zhang, Wen
Ling, Peixue
Wang, Fengshan
Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition
title Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition
title_full Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition
title_fullStr Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition
title_full_unstemmed Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition
title_short Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition
title_sort anti-inflammatory effects of a mytilus coruscus α-d-glucan (mp-a) in activated macrophage cells via tlr4/nf-κb/mapk pathway inhibition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618433/
https://www.ncbi.nlm.nih.gov/pubmed/28930149
http://dx.doi.org/10.3390/md15090294
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