Cargando…
Measurement of Telomere Length in Colorectal Cancers for Improved Molecular Diagnosis
All tumors have in common to reactivate a telomere maintenance mechanism to allow for unlimited proliferation. On the other hand, genetic instability found in some tumors can result from the loss of telomeres. Here, we measured telomere length in colorectal cancers (CRCs) using TRF (Telomere Restric...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618520/ https://www.ncbi.nlm.nih.gov/pubmed/28850092 http://dx.doi.org/10.3390/ijms18091871 |
_version_ | 1783267205369036800 |
---|---|
author | Le Balc’h, Eric Grandin, Nathalie Demattei, Marie-Véronique Guyétant, Serge Tallet, Anne Pagès, Jean-Christophe Ouaissi, Mehdi Lecomte, Thierry Charbonneau, Michel |
author_facet | Le Balc’h, Eric Grandin, Nathalie Demattei, Marie-Véronique Guyétant, Serge Tallet, Anne Pagès, Jean-Christophe Ouaissi, Mehdi Lecomte, Thierry Charbonneau, Michel |
author_sort | Le Balc’h, Eric |
collection | PubMed |
description | All tumors have in common to reactivate a telomere maintenance mechanism to allow for unlimited proliferation. On the other hand, genetic instability found in some tumors can result from the loss of telomeres. Here, we measured telomere length in colorectal cancers (CRCs) using TRF (Telomere Restriction Fragment) analysis. Telomeric DNA content was also quantified as the ratio of total telomeric (TTAGGG) sequences over that of the invariable Alu sequences. In most of the 125 CRCs analyzed, there was a significant diminution in telomere length compared with that in control healthy tissue. Only 34 tumors exhibited no telomere erosion and, in some cases, a slight telomere lengthening. Telomere length did not correlate with age, gender, tumor stage, tumor localization or stage of tumor differentiation. In addition, while telomere length did not correlate with the presence of a mutation in BRAF (V-raf murine sarcoma viral oncogene homolog B), PIK3CA (phosphatidylinositol 3-kinase catalytic subunit), or MSI status, it was significantly associated with the occurrence of a mutation in KRAS. Interestingly, we found that the shorter the telomeres in healthy tissue of a patient, the larger an increase in telomere length in the tumor. Our study points to the existence of two types of CRCs based on telomere length and reveals that telomere length in healthy tissue might influence telomere maintenance mechanisms in the tumor. |
format | Online Article Text |
id | pubmed-5618520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56185202017-09-30 Measurement of Telomere Length in Colorectal Cancers for Improved Molecular Diagnosis Le Balc’h, Eric Grandin, Nathalie Demattei, Marie-Véronique Guyétant, Serge Tallet, Anne Pagès, Jean-Christophe Ouaissi, Mehdi Lecomte, Thierry Charbonneau, Michel Int J Mol Sci Article All tumors have in common to reactivate a telomere maintenance mechanism to allow for unlimited proliferation. On the other hand, genetic instability found in some tumors can result from the loss of telomeres. Here, we measured telomere length in colorectal cancers (CRCs) using TRF (Telomere Restriction Fragment) analysis. Telomeric DNA content was also quantified as the ratio of total telomeric (TTAGGG) sequences over that of the invariable Alu sequences. In most of the 125 CRCs analyzed, there was a significant diminution in telomere length compared with that in control healthy tissue. Only 34 tumors exhibited no telomere erosion and, in some cases, a slight telomere lengthening. Telomere length did not correlate with age, gender, tumor stage, tumor localization or stage of tumor differentiation. In addition, while telomere length did not correlate with the presence of a mutation in BRAF (V-raf murine sarcoma viral oncogene homolog B), PIK3CA (phosphatidylinositol 3-kinase catalytic subunit), or MSI status, it was significantly associated with the occurrence of a mutation in KRAS. Interestingly, we found that the shorter the telomeres in healthy tissue of a patient, the larger an increase in telomere length in the tumor. Our study points to the existence of two types of CRCs based on telomere length and reveals that telomere length in healthy tissue might influence telomere maintenance mechanisms in the tumor. MDPI 2017-08-29 /pmc/articles/PMC5618520/ /pubmed/28850092 http://dx.doi.org/10.3390/ijms18091871 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Le Balc’h, Eric Grandin, Nathalie Demattei, Marie-Véronique Guyétant, Serge Tallet, Anne Pagès, Jean-Christophe Ouaissi, Mehdi Lecomte, Thierry Charbonneau, Michel Measurement of Telomere Length in Colorectal Cancers for Improved Molecular Diagnosis |
title | Measurement of Telomere Length in Colorectal Cancers for Improved Molecular Diagnosis |
title_full | Measurement of Telomere Length in Colorectal Cancers for Improved Molecular Diagnosis |
title_fullStr | Measurement of Telomere Length in Colorectal Cancers for Improved Molecular Diagnosis |
title_full_unstemmed | Measurement of Telomere Length in Colorectal Cancers for Improved Molecular Diagnosis |
title_short | Measurement of Telomere Length in Colorectal Cancers for Improved Molecular Diagnosis |
title_sort | measurement of telomere length in colorectal cancers for improved molecular diagnosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618520/ https://www.ncbi.nlm.nih.gov/pubmed/28850092 http://dx.doi.org/10.3390/ijms18091871 |
work_keys_str_mv | AT lebalcheric measurementoftelomerelengthincolorectalcancersforimprovedmoleculardiagnosis AT grandinnathalie measurementoftelomerelengthincolorectalcancersforimprovedmoleculardiagnosis AT dematteimarieveronique measurementoftelomerelengthincolorectalcancersforimprovedmoleculardiagnosis AT guyetantserge measurementoftelomerelengthincolorectalcancersforimprovedmoleculardiagnosis AT talletanne measurementoftelomerelengthincolorectalcancersforimprovedmoleculardiagnosis AT pagesjeanchristophe measurementoftelomerelengthincolorectalcancersforimprovedmoleculardiagnosis AT ouaissimehdi measurementoftelomerelengthincolorectalcancersforimprovedmoleculardiagnosis AT lecomtethierry measurementoftelomerelengthincolorectalcancersforimprovedmoleculardiagnosis AT charbonneaumichel measurementoftelomerelengthincolorectalcancersforimprovedmoleculardiagnosis |