Cargando…

3D Printed, Microgroove Pattern-Driven Generation of Oriented Ligamentous Architectures

Specific orientations of regenerated ligaments are crucially required for mechanoresponsive properties and various biomechanical adaptations, which are the key interplay to support mineralized tissues. Although various 2D platforms or 3D printing systems can guide cellular activities or aligned orga...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Chan Ho, Kim, Kyoung-Hwa, Lee, Yong-Moo, Giannobile, William V., Seol, Yang-Jo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618576/
https://www.ncbi.nlm.nih.gov/pubmed/28885543
http://dx.doi.org/10.3390/ijms18091927
_version_ 1783267218862112768
author Park, Chan Ho
Kim, Kyoung-Hwa
Lee, Yong-Moo
Giannobile, William V.
Seol, Yang-Jo
author_facet Park, Chan Ho
Kim, Kyoung-Hwa
Lee, Yong-Moo
Giannobile, William V.
Seol, Yang-Jo
author_sort Park, Chan Ho
collection PubMed
description Specific orientations of regenerated ligaments are crucially required for mechanoresponsive properties and various biomechanical adaptations, which are the key interplay to support mineralized tissues. Although various 2D platforms or 3D printing systems can guide cellular activities or aligned organizations, it remains a challenge to develop ligament-guided, 3D architectures with the angular controllability for parallel, oblique or perpendicular orientations of cells required for biomechanical support of organs. Here, we show the use of scaffold design by additive manufacturing for specific topographies or angulated microgroove patterns to control cell orientations such as parallel (0°), oblique (45°) and perpendicular (90°) angulations. These results demonstrate that ligament cells displayed highly predictable and controllable orientations along microgroove patterns on 3D biopolymeric scaffolds. Our findings demonstrate that 3D printed topographical approaches can regulate spatiotemporal cell organizations that offer strong potential for adaptation to complex tissue defects to regenerate ligament-bone complexes.
format Online
Article
Text
id pubmed-5618576
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-56185762017-09-30 3D Printed, Microgroove Pattern-Driven Generation of Oriented Ligamentous Architectures Park, Chan Ho Kim, Kyoung-Hwa Lee, Yong-Moo Giannobile, William V. Seol, Yang-Jo Int J Mol Sci Article Specific orientations of regenerated ligaments are crucially required for mechanoresponsive properties and various biomechanical adaptations, which are the key interplay to support mineralized tissues. Although various 2D platforms or 3D printing systems can guide cellular activities or aligned organizations, it remains a challenge to develop ligament-guided, 3D architectures with the angular controllability for parallel, oblique or perpendicular orientations of cells required for biomechanical support of organs. Here, we show the use of scaffold design by additive manufacturing for specific topographies or angulated microgroove patterns to control cell orientations such as parallel (0°), oblique (45°) and perpendicular (90°) angulations. These results demonstrate that ligament cells displayed highly predictable and controllable orientations along microgroove patterns on 3D biopolymeric scaffolds. Our findings demonstrate that 3D printed topographical approaches can regulate spatiotemporal cell organizations that offer strong potential for adaptation to complex tissue defects to regenerate ligament-bone complexes. MDPI 2017-09-08 /pmc/articles/PMC5618576/ /pubmed/28885543 http://dx.doi.org/10.3390/ijms18091927 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Chan Ho
Kim, Kyoung-Hwa
Lee, Yong-Moo
Giannobile, William V.
Seol, Yang-Jo
3D Printed, Microgroove Pattern-Driven Generation of Oriented Ligamentous Architectures
title 3D Printed, Microgroove Pattern-Driven Generation of Oriented Ligamentous Architectures
title_full 3D Printed, Microgroove Pattern-Driven Generation of Oriented Ligamentous Architectures
title_fullStr 3D Printed, Microgroove Pattern-Driven Generation of Oriented Ligamentous Architectures
title_full_unstemmed 3D Printed, Microgroove Pattern-Driven Generation of Oriented Ligamentous Architectures
title_short 3D Printed, Microgroove Pattern-Driven Generation of Oriented Ligamentous Architectures
title_sort 3d printed, microgroove pattern-driven generation of oriented ligamentous architectures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618576/
https://www.ncbi.nlm.nih.gov/pubmed/28885543
http://dx.doi.org/10.3390/ijms18091927
work_keys_str_mv AT parkchanho 3dprintedmicrogroovepatterndrivengenerationoforientedligamentousarchitectures
AT kimkyounghwa 3dprintedmicrogroovepatterndrivengenerationoforientedligamentousarchitectures
AT leeyongmoo 3dprintedmicrogroovepatterndrivengenerationoforientedligamentousarchitectures
AT giannobilewilliamv 3dprintedmicrogroovepatterndrivengenerationoforientedligamentousarchitectures
AT seolyangjo 3dprintedmicrogroovepatterndrivengenerationoforientedligamentousarchitectures