Cargando…
Therapeutic Effect of Cilostazol Ophthalmic Nanodispersions on Retinal Dysfunction in Streptozotocin-Induced Diabetic Rats
We previously prepared ophthalmic formulations containing cilostazol (CLZ) nanoparticles by bead mill methods (CLZ(nano)), and found that instillation of CLZ(nano) into rat eyes supplies CLZ into the retina. In this study, we investigated changes in the electroretinograms (ERG) of streptozotocin-ind...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618620/ https://www.ncbi.nlm.nih.gov/pubmed/28906472 http://dx.doi.org/10.3390/ijms18091971 |
_version_ | 1783267229573316608 |
---|---|
author | Nagai, Noriaki Deguchi, Saori Otake, Hiroko Hiramatsu, Noriko Yamamoto, Naoki |
author_facet | Nagai, Noriaki Deguchi, Saori Otake, Hiroko Hiramatsu, Noriko Yamamoto, Naoki |
author_sort | Nagai, Noriaki |
collection | PubMed |
description | We previously prepared ophthalmic formulations containing cilostazol (CLZ) nanoparticles by bead mill methods (CLZ(nano)), and found that instillation of CLZ(nano) into rat eyes supplies CLZ into the retina. In this study, we investigated changes in the electroretinograms (ERG) of streptozotocin-induced diabetic rats (STZ rats), a model of diabetes mellitus. In addition, we demonstrated that dispersions containing CLZ nanoparticles attenuate changes in the ERG of STZ rats. The instillation of CLZ(nano) had no effect on body weight or plasma glucose and insulin levels. Furthermore, no corneal toxicity was observed in the in vivo study using STZ rats. The a-wave and b-wave levels in addition to oscillatory potentials (OP) amplitude decreased in STZ rats two weeks after the injection of streptozotocin, with the instillation of CLZ(nano) attenuating these decreases. In addition, the level of vascular endothelial growth factor (VEGF) in the retinas of STZ rats was 9.26-fold higher than in in normal rats, with this increase also prevented by the instillation of CLZ(nano) Thus, we have found that a-wave and b-wave levels in addition to OP amplitude are decreased in rats following the injection of excessive streptozotocin. Furthermore, the retinal disorders associated with diabetes mellitus are attenuated by the instillation of CLZ(nano). These findings provide significant information that can be used to design further studies aimed at developing anti-diabetic retinopathy drugs. |
format | Online Article Text |
id | pubmed-5618620 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56186202017-09-30 Therapeutic Effect of Cilostazol Ophthalmic Nanodispersions on Retinal Dysfunction in Streptozotocin-Induced Diabetic Rats Nagai, Noriaki Deguchi, Saori Otake, Hiroko Hiramatsu, Noriko Yamamoto, Naoki Int J Mol Sci Article We previously prepared ophthalmic formulations containing cilostazol (CLZ) nanoparticles by bead mill methods (CLZ(nano)), and found that instillation of CLZ(nano) into rat eyes supplies CLZ into the retina. In this study, we investigated changes in the electroretinograms (ERG) of streptozotocin-induced diabetic rats (STZ rats), a model of diabetes mellitus. In addition, we demonstrated that dispersions containing CLZ nanoparticles attenuate changes in the ERG of STZ rats. The instillation of CLZ(nano) had no effect on body weight or plasma glucose and insulin levels. Furthermore, no corneal toxicity was observed in the in vivo study using STZ rats. The a-wave and b-wave levels in addition to oscillatory potentials (OP) amplitude decreased in STZ rats two weeks after the injection of streptozotocin, with the instillation of CLZ(nano) attenuating these decreases. In addition, the level of vascular endothelial growth factor (VEGF) in the retinas of STZ rats was 9.26-fold higher than in in normal rats, with this increase also prevented by the instillation of CLZ(nano) Thus, we have found that a-wave and b-wave levels in addition to OP amplitude are decreased in rats following the injection of excessive streptozotocin. Furthermore, the retinal disorders associated with diabetes mellitus are attenuated by the instillation of CLZ(nano). These findings provide significant information that can be used to design further studies aimed at developing anti-diabetic retinopathy drugs. MDPI 2017-09-14 /pmc/articles/PMC5618620/ /pubmed/28906472 http://dx.doi.org/10.3390/ijms18091971 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nagai, Noriaki Deguchi, Saori Otake, Hiroko Hiramatsu, Noriko Yamamoto, Naoki Therapeutic Effect of Cilostazol Ophthalmic Nanodispersions on Retinal Dysfunction in Streptozotocin-Induced Diabetic Rats |
title | Therapeutic Effect of Cilostazol Ophthalmic Nanodispersions on Retinal Dysfunction in Streptozotocin-Induced Diabetic Rats |
title_full | Therapeutic Effect of Cilostazol Ophthalmic Nanodispersions on Retinal Dysfunction in Streptozotocin-Induced Diabetic Rats |
title_fullStr | Therapeutic Effect of Cilostazol Ophthalmic Nanodispersions on Retinal Dysfunction in Streptozotocin-Induced Diabetic Rats |
title_full_unstemmed | Therapeutic Effect of Cilostazol Ophthalmic Nanodispersions on Retinal Dysfunction in Streptozotocin-Induced Diabetic Rats |
title_short | Therapeutic Effect of Cilostazol Ophthalmic Nanodispersions on Retinal Dysfunction in Streptozotocin-Induced Diabetic Rats |
title_sort | therapeutic effect of cilostazol ophthalmic nanodispersions on retinal dysfunction in streptozotocin-induced diabetic rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618620/ https://www.ncbi.nlm.nih.gov/pubmed/28906472 http://dx.doi.org/10.3390/ijms18091971 |
work_keys_str_mv | AT nagainoriaki therapeuticeffectofcilostazolophthalmicnanodispersionsonretinaldysfunctioninstreptozotocininduceddiabeticrats AT deguchisaori therapeuticeffectofcilostazolophthalmicnanodispersionsonretinaldysfunctioninstreptozotocininduceddiabeticrats AT otakehiroko therapeuticeffectofcilostazolophthalmicnanodispersionsonretinaldysfunctioninstreptozotocininduceddiabeticrats AT hiramatsunoriko therapeuticeffectofcilostazolophthalmicnanodispersionsonretinaldysfunctioninstreptozotocininduceddiabeticrats AT yamamotonaoki therapeuticeffectofcilostazolophthalmicnanodispersionsonretinaldysfunctioninstreptozotocininduceddiabeticrats |