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Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold
Optic neuropathies, such as glaucoma and Leber’s hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correc...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618661/ https://www.ncbi.nlm.nih.gov/pubmed/28930148 http://dx.doi.org/10.3390/ijms18092013 |
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author | Yang, Tien-Chun Chuang, Jen-Hua Buddhakosai, Waradee Wu, Wen-Ju Lee, Chen-Ju Chen, Wun-Syuan Yang, Yi-Ping Li, Ming-Chia Peng, Chi-Hsien Chen, Shih-Jen |
author_facet | Yang, Tien-Chun Chuang, Jen-Hua Buddhakosai, Waradee Wu, Wen-Ju Lee, Chen-Ju Chen, Wun-Syuan Yang, Yi-Ping Li, Ming-Chia Peng, Chi-Hsien Chen, Shih-Jen |
author_sort | Yang, Tien-Chun |
collection | PubMed |
description | Optic neuropathies, such as glaucoma and Leber’s hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correct location of the retina. The use of appropriate scaffold can promote the proper axon growth. Recently, biocompatible materials have been integrated into the medical field, such as tissue engineering and reconstruction of damaged tissues or organs. We, herein, utilized nano-imprinting to create a scaffold mimicking the in vitro tissue microarchitecture, and guiding the axonal growth and orientation of the RGCs. We observed that the robust, long, and organized axons of human induced pluripotent stem cell (iPSC)-derived RGCs projected axially along the scaffold grooves. The RGCs grown on the scaffold expressed the specific neuronal biomarkers indicating their proper functionality. Thus, based on our in vitro culture system, this device can be useful for the neurophysiological analysis and transplantation for ophthalmic neuropathy treatment. |
format | Online Article Text |
id | pubmed-5618661 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56186612017-09-30 Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold Yang, Tien-Chun Chuang, Jen-Hua Buddhakosai, Waradee Wu, Wen-Ju Lee, Chen-Ju Chen, Wun-Syuan Yang, Yi-Ping Li, Ming-Chia Peng, Chi-Hsien Chen, Shih-Jen Int J Mol Sci Article Optic neuropathies, such as glaucoma and Leber’s hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correct location of the retina. The use of appropriate scaffold can promote the proper axon growth. Recently, biocompatible materials have been integrated into the medical field, such as tissue engineering and reconstruction of damaged tissues or organs. We, herein, utilized nano-imprinting to create a scaffold mimicking the in vitro tissue microarchitecture, and guiding the axonal growth and orientation of the RGCs. We observed that the robust, long, and organized axons of human induced pluripotent stem cell (iPSC)-derived RGCs projected axially along the scaffold grooves. The RGCs grown on the scaffold expressed the specific neuronal biomarkers indicating their proper functionality. Thus, based on our in vitro culture system, this device can be useful for the neurophysiological analysis and transplantation for ophthalmic neuropathy treatment. MDPI 2017-09-20 /pmc/articles/PMC5618661/ /pubmed/28930148 http://dx.doi.org/10.3390/ijms18092013 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Tien-Chun Chuang, Jen-Hua Buddhakosai, Waradee Wu, Wen-Ju Lee, Chen-Ju Chen, Wun-Syuan Yang, Yi-Ping Li, Ming-Chia Peng, Chi-Hsien Chen, Shih-Jen Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold |
title | Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold |
title_full | Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold |
title_fullStr | Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold |
title_full_unstemmed | Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold |
title_short | Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold |
title_sort | elongation of axon extension for human ipsc-derived retinal ganglion cells by a nano-imprinted scaffold |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618661/ https://www.ncbi.nlm.nih.gov/pubmed/28930148 http://dx.doi.org/10.3390/ijms18092013 |
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