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Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold

Optic neuropathies, such as glaucoma and Leber’s hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correc...

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Autores principales: Yang, Tien-Chun, Chuang, Jen-Hua, Buddhakosai, Waradee, Wu, Wen-Ju, Lee, Chen-Ju, Chen, Wun-Syuan, Yang, Yi-Ping, Li, Ming-Chia, Peng, Chi-Hsien, Chen, Shih-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618661/
https://www.ncbi.nlm.nih.gov/pubmed/28930148
http://dx.doi.org/10.3390/ijms18092013
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author Yang, Tien-Chun
Chuang, Jen-Hua
Buddhakosai, Waradee
Wu, Wen-Ju
Lee, Chen-Ju
Chen, Wun-Syuan
Yang, Yi-Ping
Li, Ming-Chia
Peng, Chi-Hsien
Chen, Shih-Jen
author_facet Yang, Tien-Chun
Chuang, Jen-Hua
Buddhakosai, Waradee
Wu, Wen-Ju
Lee, Chen-Ju
Chen, Wun-Syuan
Yang, Yi-Ping
Li, Ming-Chia
Peng, Chi-Hsien
Chen, Shih-Jen
author_sort Yang, Tien-Chun
collection PubMed
description Optic neuropathies, such as glaucoma and Leber’s hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correct location of the retina. The use of appropriate scaffold can promote the proper axon growth. Recently, biocompatible materials have been integrated into the medical field, such as tissue engineering and reconstruction of damaged tissues or organs. We, herein, utilized nano-imprinting to create a scaffold mimicking the in vitro tissue microarchitecture, and guiding the axonal growth and orientation of the RGCs. We observed that the robust, long, and organized axons of human induced pluripotent stem cell (iPSC)-derived RGCs projected axially along the scaffold grooves. The RGCs grown on the scaffold expressed the specific neuronal biomarkers indicating their proper functionality. Thus, based on our in vitro culture system, this device can be useful for the neurophysiological analysis and transplantation for ophthalmic neuropathy treatment.
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spelling pubmed-56186612017-09-30 Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold Yang, Tien-Chun Chuang, Jen-Hua Buddhakosai, Waradee Wu, Wen-Ju Lee, Chen-Ju Chen, Wun-Syuan Yang, Yi-Ping Li, Ming-Chia Peng, Chi-Hsien Chen, Shih-Jen Int J Mol Sci Article Optic neuropathies, such as glaucoma and Leber’s hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correct location of the retina. The use of appropriate scaffold can promote the proper axon growth. Recently, biocompatible materials have been integrated into the medical field, such as tissue engineering and reconstruction of damaged tissues or organs. We, herein, utilized nano-imprinting to create a scaffold mimicking the in vitro tissue microarchitecture, and guiding the axonal growth and orientation of the RGCs. We observed that the robust, long, and organized axons of human induced pluripotent stem cell (iPSC)-derived RGCs projected axially along the scaffold grooves. The RGCs grown on the scaffold expressed the specific neuronal biomarkers indicating their proper functionality. Thus, based on our in vitro culture system, this device can be useful for the neurophysiological analysis and transplantation for ophthalmic neuropathy treatment. MDPI 2017-09-20 /pmc/articles/PMC5618661/ /pubmed/28930148 http://dx.doi.org/10.3390/ijms18092013 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Tien-Chun
Chuang, Jen-Hua
Buddhakosai, Waradee
Wu, Wen-Ju
Lee, Chen-Ju
Chen, Wun-Syuan
Yang, Yi-Ping
Li, Ming-Chia
Peng, Chi-Hsien
Chen, Shih-Jen
Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold
title Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold
title_full Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold
title_fullStr Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold
title_full_unstemmed Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold
title_short Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold
title_sort elongation of axon extension for human ipsc-derived retinal ganglion cells by a nano-imprinted scaffold
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618661/
https://www.ncbi.nlm.nih.gov/pubmed/28930148
http://dx.doi.org/10.3390/ijms18092013
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