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Culture conditions defining glioblastoma cells behavior: what is the impact for novel discoveries?

In cancer research, the use of established cell lines has gradually been replaced by primary cell cultures due to their better representation of in vivo cancer cell behaviors. However, a major challenge with primary culture involves the finding of growth conditions that minimize alterations in the b...

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Autores principales: Ledur, Pítia Flores, Onzi, Giovana Ravizzoni, Zong, Hui, Lenz, Guido
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620329/
https://www.ncbi.nlm.nih.gov/pubmed/28978189
http://dx.doi.org/10.18632/oncotarget.20193
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author Ledur, Pítia Flores
Onzi, Giovana Ravizzoni
Zong, Hui
Lenz, Guido
author_facet Ledur, Pítia Flores
Onzi, Giovana Ravizzoni
Zong, Hui
Lenz, Guido
author_sort Ledur, Pítia Flores
collection PubMed
description In cancer research, the use of established cell lines has gradually been replaced by primary cell cultures due to their better representation of in vivo cancer cell behaviors. However, a major challenge with primary culture involves the finding of growth conditions that minimize alterations in the biological state of the cells. To ensure reproducibility and translational potentials for research findings, culture conditions need to be chosen so that the cell population in culture best mimics tumor cells in vivo. Glioblastoma (GBM) is one of the most aggressive and heterogeneous tumor types and the GBM research field would certainly benefit from culture conditions that could maintain the original plethora of phenotype of the cells. Here, we review culture media and supplementation options for GBM cultures, the rationale behind their use, and how much those choices affect drug-screening outcomes. We provide an overview of 120 papers that use primary GBM cultures and discuss the current predominant conditions. We also show important primary research data indicating that “mis-cultured” glioma cells can acquire unnatural drug sensitivity, which would have devastating effects for clinical translations. Finally, we propose the concurrent test of four culture conditions to minimize the loss of cell coverage in culture.
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spelling pubmed-56203292017-10-03 Culture conditions defining glioblastoma cells behavior: what is the impact for novel discoveries? Ledur, Pítia Flores Onzi, Giovana Ravizzoni Zong, Hui Lenz, Guido Oncotarget Review In cancer research, the use of established cell lines has gradually been replaced by primary cell cultures due to their better representation of in vivo cancer cell behaviors. However, a major challenge with primary culture involves the finding of growth conditions that minimize alterations in the biological state of the cells. To ensure reproducibility and translational potentials for research findings, culture conditions need to be chosen so that the cell population in culture best mimics tumor cells in vivo. Glioblastoma (GBM) is one of the most aggressive and heterogeneous tumor types and the GBM research field would certainly benefit from culture conditions that could maintain the original plethora of phenotype of the cells. Here, we review culture media and supplementation options for GBM cultures, the rationale behind their use, and how much those choices affect drug-screening outcomes. We provide an overview of 120 papers that use primary GBM cultures and discuss the current predominant conditions. We also show important primary research data indicating that “mis-cultured” glioma cells can acquire unnatural drug sensitivity, which would have devastating effects for clinical translations. Finally, we propose the concurrent test of four culture conditions to minimize the loss of cell coverage in culture. Impact Journals LLC 2017-08-11 /pmc/articles/PMC5620329/ /pubmed/28978189 http://dx.doi.org/10.18632/oncotarget.20193 Text en Copyright: © 2017 Ledur et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Review
Ledur, Pítia Flores
Onzi, Giovana Ravizzoni
Zong, Hui
Lenz, Guido
Culture conditions defining glioblastoma cells behavior: what is the impact for novel discoveries?
title Culture conditions defining glioblastoma cells behavior: what is the impact for novel discoveries?
title_full Culture conditions defining glioblastoma cells behavior: what is the impact for novel discoveries?
title_fullStr Culture conditions defining glioblastoma cells behavior: what is the impact for novel discoveries?
title_full_unstemmed Culture conditions defining glioblastoma cells behavior: what is the impact for novel discoveries?
title_short Culture conditions defining glioblastoma cells behavior: what is the impact for novel discoveries?
title_sort culture conditions defining glioblastoma cells behavior: what is the impact for novel discoveries?
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620329/
https://www.ncbi.nlm.nih.gov/pubmed/28978189
http://dx.doi.org/10.18632/oncotarget.20193
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